复方中药提取物对公兔生殖生理的影响

doi:10.16431/j.cnki.1671-7236.2024.03.024

摘要/Abstract

摘要： 【目的】讨论不同剂量复方中药提取物对公兔精液品质、生殖激素、睾丸形态及精子顶体完整性的影响,为提高公兔的繁殖性能及研制此类功能的中药饲料添加剂提供理论依据。【方法】选取24只新西兰公兔,随机分为4组,每组6只,空白对照组公兔不添加复方中药提取物,试验Ⅰ、Ⅱ和Ⅲ组公兔分别添加150、300、600 mg/kg复方中药提取物。试验周期为12周,饲喂周期为8周。分别于试验第4、8、12周采集血液样本,于试验第8、10、12周采集精液样本,进行血清中性激素的测定与精液品质鉴定。【结果】与对照组相比,8~12周试验组兔精子密度、精子活率、A级精子数量均显著升高(P＜0.05),试验Ⅲ组兔精子顶体完整率显著升高(P＜0.05);10~12周试验Ⅲ组兔精子畸形率显著降低(P＜0.05)。试验组兔睾丸组织表面被膜由较厚的不规则致密结缔组织构成,睾丸内大量曲精小管基底膜清晰可见,曲精小管上皮是由生精细胞与支持细胞构成的复层上皮,曲精小管上皮分布大量精原细胞和分化的精母细胞,管腔内可见数量丰富的精子,间质无明显异常。4~12周试验Ⅱ、Ⅲ组兔血清中促卵泡素(FSH)、黄体生成素(LH)、睾酮(T)含量均显著高于对照组(P＜0.05)。【结论】复方中药提取物可明显改善公兔精液品质,提高精子顶体完整率,降低精子畸形率;有效改善睾丸形态,促进生殖器官发育;提高血清中FSH、LH、T生殖激素含量。其中600 mg/kg复方中药提取物对于公兔的生殖生理具有显著影响,在实际生产中具有良好的应用前景。

关键词: 复方中药提取物; 公兔; 睾丸; 精液品质; 生殖激素

Abstract: 【Objective】 This study was aimed to discuss the effects of different doses of compound Chinese medicine extract on semen quality,reproductive hormone,testis morphology and sperm acrosome integrity in male rabbits,and provide a theoretical basis for improving reproductive performance of male rabbits and developing traditional Chinese medicine feed additives with this kind of function.【Method】 Twenty-four New Zealand male rabbits were randomly divided into 4 groups,with 6 rabbits in each group.The male rabbits in control,Ⅰ,Ⅱ and Ⅲ groups were given 0,150,300 and 600 mg/kg compound Chinese medicine extract,respectively.The experimental period was 12 weeks,and the feeding period was 8 weeks.Blood samples were collected at the 4th,8th and 12th week,and the semen samples were collected at the 8th,10th and 12th week for the determination of serum sex hormone and semen quality.【Result】 Compared with control group,the sperm density,sperm viability and the number of grade A sperm of rabbits in experimental group at 8-12 weeks were significantly increased (P＜0.05),and the rate of acrosome integrity of rabbits in Ⅲ group was significantly increased (P＜0.05).The sperm deformity rate of rabbits in Ⅲ group was significantly lower than that in control group at 10-12 weeks (P＜0.05).The surface capsule of the testicular tissue of rabbits in experimental group was composed of thick irregular dense connective tissue and a large number of basement membranes of seminiferous tubules were clearly visible,the epithelium of seminiferous tubule was composed of spermatogenic cells and Sertoli cells.There were a lot of spermatogonia and differentiated spermatocytes in the epithelium of seminiferous tubule.There are abundant sperm in lumen and no obvious abnormalities in stroma.At 4-12 weeks,The contents of follicle stimulating hormone (FSH),luteinizing hormone (LH) and testosterone (T) of serum in Ⅱ and Ⅲ groups were significantly higher than that in control group (P＜0.05).【Conclusion】 Compound Chinese medicine extract could improve the quality of male rabbit semen,increase the rate of sperm acrosome integrity,reduce the sperm deformity rate,improve the morphology of testis,promote the development of reproductive organs,and increase the contents of FSH,LH and T in serum.The 600 mg/kg compound Chinese medicine extract had a significant effect on reproductive physiology of male rabbits,and had a good application prospect in practical production.

Key words: compound Chinese medicine extract; male rabbits; testicular; semen quality; reproductive hormones

中图分类号:

S814.1

王士勇, 苏东雪, 张靖, 陈丽红, 詹军, 陈尧, 文盛鸿, 杨月春. 复方中药提取物对公兔生殖生理的影响[J]. 中国畜牧兽医, 2024, 51(3): 1122-1131.

WANG Shiyong, SU Dongxue, ZHANG Jing, CHEN Lihong, ZHAN Jun, CHEN Yao, WEN Shenghong, YANG Yuechun. Effect of Compound Chinese Medicine Extract on Reproductive Physiology of Male Rabbits[J]. China Animal Husbandry and Veterinary Medicine, 2024, 51(3): 1122-1131.

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