丁酸梭菌和丁酸钠对高脂饮食诱导雄性小鼠生殖损伤的影响

doi:10.16431/j.cnki.1671-7236.2023.04.032

摘要/Abstract

摘要： 【目的】探讨丁酸梭菌和丁酸钠对高脂饮食刺激下小鼠精液品质和睾丸组织的影响,为治疗雄性生殖疾病提供试验基础和理论支持。【方法】试验1:将32只8周龄C57BL/6雄性小鼠随机均分为4组,对照组(NC)饲喂基础饲粮,高脂组(HFD)、丁酸梭菌处理组(HFD+C.butyricum)和丁酸钠处理组(HFD+Sodium butyrate)均饲喂含60%脂肪的高脂饲料,对照组和高脂组灌胃生理盐水,丁酸梭菌处理组灌胃1×10⁸CFU/kg丁酸梭菌,丁酸钠处理组灌胃300 mg/kg丁酸钠,每天1次。试验2:将16只8周龄C57BL/6雄性小鼠随机均分为2组,对照组正常饮食补充生理盐水,试验组小鼠通过灌胃补充1×10⁸CFU/kg丁酸梭菌溶液,每天1次,12周后处死采样。利用精液检测系统CASA检测精液品质,HE染色观察睾丸组织病理变化;利用试剂盒检测睾丸组织总抗氧化能力(T-AOC)、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)活性及丙二醛(MDA)、血清睾酮(T)含量,以及睾丸组织碱性磷酸酶(AKP)、酸性磷酸酶(ACP)、乳酸脱氢酶(LDH)及γ-谷氨酰基转移酶(γ-GT)活性。【结果】试验1中,与高脂组相比,丁酸梭菌处理组小鼠体重极显著降低(P＜0.01),小鼠精子活力、A+B级精子数、精子直线运动能力、平均路径速度均显著提高(P＜0.05),直线速度极显著提高(P＜0.01),小鼠睾丸组织AKP活性显著提高(P＜0.05);丁酸钠处理组小鼠体重极显著降低(P＜0.01),睾丸指数、精子直线运动能力、精子向前运动力、直线速度和摆动性均显著提高(P＜0.05),精子活力、A+B级精子数、平均路径速度及睾丸组织γ-GT活性均极显著提高(P＜0.01),此外,丁酸钠还显著增加了高脂小鼠血清睾酮含量、ACP活性(P＜0.05),显著降低了小鼠睾丸组织MDA含量(P＜0.05)。睾丸组织病理切片显示,各组小鼠睾丸组织形态并无明显差异。试验2中,与对照组相比,试验组小鼠睾丸指数极显著升高(P＜0.01),血清中睾酮水平显著升高(P＜0.05),精液品质及睾丸组织抗氧化指标均无显著变化(P＞0.05)。【结论】丁酸梭菌及丁酸钠可显著升高小鼠血清睾酮含量,改善睾丸组织抗氧化能力,提高精子质量和数量,保护睾丸组织结构,有效保护肥胖导致的雄性小鼠生殖系统损伤。

关键词: 丁酸梭菌; 丁酸钠; 高脂; 睾丸; 精液品质

Abstract: 【Objective】 This study was aimed to investigate the effects of Clostridium butyrate (C.butyricum) and sodium butyrate on semen quality and testicular tissue of mice stimulated by high fat diet,so as to provide experimental basis and theoretical support for the treatment of male reproductive diseases.【Method】 In experiment 1,32 8-week-old C57BL/6 male mice were randomly divided into 4 groups.The mice in control group (NC) were fed a basal diet, in high fat diet group (HFD), C. butyricum treatment group (HFD+C.butyricum) and sodium butyrate treatment group (HFD+Sodium butyrate) were fed high fat diet containing 60% fat.HFD+C.butyricum group was gavaged with 1×10⁸ CFU/kg C.butyricum,while HFD+Sodium butyrate group was gavaged with 300 mg/kg sodium butyrate,once a day.In experiment 2,16 8-week-old C57BL/6 male mice were randomly divided into two groups.The normal diet of control group was supplemented with normal saline,and the experiment group was supplemented with C.butyricum solution 1×10⁸CFU/kg by gavage,once a day.After 12 weeks,the animals were sacrificed for sampling.The semen quality was detected by CASA,and the pathological changes of testicular tissue were observed by HE staining.The total antioxidant capacity (T-AOC),the activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px),the content of malondialdehyde (MDA) in testicular tissue and serum testosterone (T), and the activities of alkaline phosphatase (AKP),acid phosphatase (ACP),lactate dehydrogenase (LDH) and gamma glutamyltransferase (γ-GT) in testicular tissue were detected by corresponding kits.【Result】 In experiment 1,compared with high-fat group,the body weight of mice in C.butyricum treatment group was extremely significantly decreased (P＜0.01),the sperm motility,the number of A+B grade sperm,sperm linear motility and the velocity of average path of mice were significantly increased (P＜0.05),and the straight-line velocity of mice was extremely significantly increased (P＜0.01).The activity of AKP in testicular tissue of mice was significantly increased (P＜0.05).Compared with high-fat group,the body weight of mice in sodium butyrate treatment group was extremely significantly decreased (P＜0.01),the testis index,sperm linear motility,sperm forward motility,straight-line velocity and swing of sperm were significantly increased (P＜0.05),and the sperm motility,the number of A+B grade sperm,the velocity of average path and the activity of γ-GT in testicular tissue were extremely significantly increased (P＜0.01).In addition,sodium butyrate significantly increased the content of serum testosterone and the activity of ACP (P＜0.05),and significantly decreased the content of MDA in testicular tissue of mice (P＜0.05).The pathological section of testicular tissue showed that there was no significant difference in testicular tissue morphology of mice in each group.In experiment 2,compared with control group,the testicular coefficient of mice treated with C.butyricum were extremely significantly increased (P＜0.01),and the level of serum testosterone was significantly increased (P＜0.05),but there were no significant change in semen quality and antioxidant indexes of testicular tissue (P＞0.05).【Conclusion】 C.butyricum and sodium butyrate could significantly increase the content of serum testosterone in mice,improve the antioxidant capacity of testicular tissue,improve the quality and quantity of sperm,protect the structure of testicular tissue,and effectively protect the reproductive system injury caused by obesity in male mice.

Key words: Clostridium butyricum; sodium butyrate; high fat; testis; semen quality

中图分类号:

S816.73

杜朝晖, 葛梓豪, 游君怡, 刘晓玮, 梁国栋, 孙世铎, 胡建宏, 李晓. 丁酸梭菌和丁酸钠对高脂饮食诱导雄性小鼠生殖损伤的影响[J]. 中国畜牧兽医, 2023, 50(4): 1603-1616.

DU Zhaohui, GE Zihao, YOU Junyi, LIU Xiaowei, LIANG Guodong, SUN Shiduo, HU Jianhong, LI Xiao. Effects of Clostridium butyricum and Sodium Butyricum on Reproductive System Injury Induced by High Fat Diet in Male Mice[J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1603-1616.

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