关中奶山羊睾丸不同发育阶段代谢物的差异分析

doi:10.16431/j.cnki.1671-7236.2022.09.019

摘要/Abstract

摘要： 【目的】试验旨在从代谢组学角度探究关中奶山羊睾丸发育代谢机制。【方法】选择1月龄断奶雄性关中奶山羊和24月龄体成熟的雄性关中奶山羊各6只，采集睾丸组织，使用液相色谱-质谱（LC-MS）技术对睾丸组织进行化学检测，通过多维和单维分析相结合的方式对两组不同代谢物进行化学检测、对比与鉴别，通过富集差异代谢物分析筛选关中奶山羊发育代谢中的潜在关键通路。【结果】在1和24月龄关中奶山羊睾丸组织中共筛选出334个差异代谢物，其中显著上调137个，显著下调197个。对前36个差异代谢物进行比较鉴定发现，分别为脂质和类脂质分子、有机酸及其衍生物、未分类化合物和苯丙烷和聚酮化合物四大类。经相关性分析发现，在1和24月龄关中奶山羊睾丸发育过程中，脂质和类脂质分子与甘油磷脂呈最大正相关，羧基及其衍生物与甘油磷脂呈最大负相关。对不同的代谢物进行富集分析，筛选出7条潜在的重要代谢通路，分别为肿瘤中的胆碱代谢、精氨酸和脯氨酸代谢、肿瘤中的中心碳代谢、铁死亡、谷胱甘肽代谢、氨酰-tRNA生物组成以及牛磺酸和亚牛磺酸代谢。【结论】本试验从代谢组学角度揭示了1和24月龄关中奶山羊睾丸的差异代谢物及代谢机制，为今后哺乳动物睾丸的发育研究奠定了基础。

关键词: 关中奶山羊; 睾丸发育; 代谢组学; 差异代谢物; 代谢通路

Abstract: 【Objective】 The purpose of this study was to explore the metabolic mechanism of testis development in Guanzhong dairy goats from the perspective of metabolomics.【Method】 Six 1 month old weaned male and six 24 months old physically mature male of Guanzhong dairy goats were selected to collect testis tissue, and the testis tissues were chemically detected by liquid chromatography mass spectrometry (LC-MS) technology.The chemical detection, comparison and identification of different metabolites in two groups were carried out through the combination of multi-dimensional and single dimensional analysis.The potential key pathways in developmental metabolism of Guanzhong dairy goats were screened out using the enrichment differential metabolite analysis.【Result】 A total of 334 differential metabolites were screened out in testis of Guanzhong dairy goats at 1 and 24 months old, of which 137 were significantly upregulated and 197 were significantly downregulated.The top 36 differential metabolites were compared and identified, which were classified into 4 categories:Lipids and lipid-like molecules, organic acids and their derivatives, unclassified compounds, and phenylpropanes and polyketides.The correlation analysis showed that during testis development of Guanzhong dairy goats from 1 and 24 months old, lipids and lipid-like molecules had the largest positive correlation with glycerophospholipids, and carboxyl groups and their derivatives had the largest negative correlation with glycerophospholipids.The enrichment analysis of different metabolites resulted in 7 potentially important metabolic pathways, which were choline metabolism, arginine and proline metabolism, central carbon metabolism in tumor, ferroptosis, glutathione metabolism, aminoacyl-tRNA biocomposition, and taurine and hypotaurine metabolism.【Conclusion】 The results of this experiment revealed that the differential metabolites and metabolic mechanisms of testis in Guanzhong dairy goats at 1 and 24 months old from the perspective of metabolomics, and provided abasis for future research on the development of mammalian testis.

Key words: Guanzhong dairy goats; testis development; metabolomics; differential metabolites; metabolic pathways

中图分类号:

Q756

王广, 邹家浩, 张永涛, 李德娴, 李雪清, 余梦琦, 陈璐, 袁宇欣, 李广. 关中奶山羊睾丸不同发育阶段代谢物的差异分析[J]. 中国畜牧兽医, 2022, 49(9): 3453-3464.

WANG Guang, ZOU Jiahao, ZHANG Yongtao, LI Dexian, LI Xueqing, YU Mengqi, CHEN Lu, YUAN Yuxin, LI Guang. Difference Analysis of Metabolites in Testis of Guanzhong Dairy Goats at Different Developmental Stages[J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(9): 3453-3464.

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