基于UPLC-MS/MS代谢组学技术分析归芪益母口服液对产后奶牛血浆代谢物的影响

doi:10.16431/j.cnki.1671-7236.2023.03.007

摘要/Abstract

摘要： 【目的】通过超高效液相色谱-质谱法(UPLC-MS/MS)代谢组学技术分析归芪益母口服液对产后奶牛血浆代谢物的影响,以阐明归芪益母口服液对产后奶牛的保健机制。【方法】选取15头体况相近、2~3胎健康待产荷斯坦奶牛,随机分为对照组(n=7)和试验组(n=8),分别在产后立即灌服饮用水和归芪益母口服液,500 mL/头,连续灌服6 d。产后7 d晨饲前采血,经预处理后采用UPLC-MS/MS上机检测,利用MetaboAnanlyst 5.0软件筛选VIP≥1、P＜0.05的差异代谢物进行代谢通路富集分析。【结果】产后7 d,试验组血浆代谢轮廓较对照组发生明显改变,筛选出16种差异代谢物,其中8种显著上调,包括蛋氨酸亚砜、磷酸肌酸、甘油磷酸胆碱、皮质醇、L-色氨酸、溶血磷脂酰胆碱(18∶2)、五羟色胺和L-精氨酸;8种显著下调,包括1-氨基环己酸、3-甲基巴豆酰甘氨酸、L-天冬酰胺、单酰甘油酯酰基(16∶1)、泛酰巯基乙胺、十八碳单烯酰胺、1-甲基鸟嘌呤和3-N-甲基-L-组氨酸。差异代谢物显著富集在甘油磷脂代谢、精氨酸和脯氨酸代谢、色氨酸代谢通路。【结论】归芪益母口服液对产后奶牛具有保健作用,可能与其调节甘油磷脂代谢、皮质醇代谢、色氨酸代谢、精氨酸和脯氨酸代谢从而发挥抗炎、抗氧化、调节食欲及肠道菌群稳态、缓解能量负平衡作用有关。

关键词: 产后奶牛; 归芪益母口服液; 代谢组学; 血浆代谢物; 通路富集

Abstract: 【Objective】 The purpose of this experiment was to analyze the effects of Guiqi Yimu oral liquid on plasma metabolites in postpartum dairy cows by UPLC-MS/MS metabolomics technology,in order to clarify the health-care mechanism of Guiqi Yimu oral liquid on postpartum dairy cows.【Method】 A total of 15 healthy Holstein dairy cows with similar body condition and 2-3 parity were selected and randomly divided into control group (n=7) and experiment group (n=8).500 mL/head Guiqi Yimu oral liquid and drinking water were administered continuously for 6 days.Blood was collected before morning feeding on 7 d postpartum.After pretreatment,UPLC-MS/MS was used for on-machine detection,and MetaboAnanlyst 5.0 software was used to screen differential metabolites with VIP≥1 and P＜0.05 for metabolic pathway enrichment analysis.【Result】 At 7 days postpartum,the plasma metabolism profile in experiment group was significantly changed compared with control group.Sixteen differential metabolites were screened out,of which eight were significantly up-regulated,including methionine sulfoxide,creatine phosphate,glycerophosphocholine,cortisol,L-tryptophan,lysophosphatidylcholine(18∶2),5-hydroxytryptamine and L-arginine;Eight were significantly down-regulated,including 1-aminocyclohexanoic acid,3-methylcrotonyl glycine,L-asparagine anhydrous,monoacylglycerolacyl(16∶1),panthenyl mercaptoethylamine,octadecy monoenamide,1-methylguanine and 3-N-methyl-L-histidine.Differential metabolites were significantly enriched in glycerophospholipid metabolism,arginine and proline metabolism,and tryptophan metabolism pathways.【Conclusion】 Guiqi Yimu oral liquid had the health-care effect of postpartum dairy cows,which might play the role of anti-inflammatory,antioxidant,appetite-regulating,intestinal bacteria and relieving negative energy balance by regulating glycerophospholipid metabolism,cortisol metabolism,tryptophan metabolism,arginine and proline metabolism.

Key words: postpartum dairy cows; Guiqi Yimu oral liquid; metabolomics; plasma metabolites; pathway enrichment

中图分类号:

Q493.2

孔维怡, 王菲菲, 岳康, 秦俊杰, 朱浩, 郭延生. 基于UPLC-MS/MS代谢组学技术分析归芪益母口服液对产后奶牛血浆代谢物的影响[J]. 中国畜牧兽医, 2023, 50(3): 913-923.

KONG Weiyi, WANG Feifei, YUE Kang, QIN Junjie, ZHU Hao, GUO Yansheng. Effect of Guiqi Yimu Oral Liquid on Plasma Metabolites in Postpartum Dairy Cows Based on UPLC-MS/MS Metabolomics Technology[J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(3): 913-923.

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