基于LC-MS代谢组学的植物乳杆菌发酵黄芪的代谢产物分析

doi:10.16431/j.cnki.1671-7236.2021.09.019

摘要/Abstract

摘要： 试验旨在利用色谱-质谱联用（liquid chromatograph-mass spectrometer，LC-MS）代谢组学技术分析植物乳杆菌发酵黄芪的代谢产物，并探索其互作发酵机制。分别采取植物乳杆菌发酵黄芪（FT组）和未发酵黄芪固态粉末（CT组），经样本前处理、LC-MS分析、生物学信息分析等探寻差异代谢物并分析代谢通路。结果显示，总离子流图峰图重现性良好，发酵黄芪主要代谢物共鉴定出183种代谢成分，正离子和负离子模式样品间关系PCA图均能良好区分。火山图分析表明，FT和CT组间的代谢物变化具有差异性，正离子模式上调的1 416个代谢物富集到83个代谢途径；下调的935个代谢物富集到83个代谢途径；负离子模式上调的1 040个代谢物富集到52个代谢途径，下调的809个代谢物富集到45个代谢途径。发酵黄芪差异代谢产物酸类、脂类、酮类等氨基酸显著增加，烯类等氨基酸显著下调，其中上调代谢物15个，下调代谢物2个，关键代谢产物主要为α-硫酸二乙酯、2-甲基柠檬酸、3-异丙烯基-6-氧代庚酸等，涉及到丙酮酸代谢、丙酸代谢、半乳糖代谢等途径。本研究结果为发酵黄芪的代谢产物、发酵互作机制和临床应用提供理论依据。

关键词: 植物乳杆菌; 黄芪; 发酵; LC-MS; 代谢组学

Abstract: This study was aimed to analyze the metabolites of fermented Astragalus by Lactobacillus plantarum using liquid chromatograph-mass spectrometer (LC-MS) metabonomics technology, and explore the mechanism of its interaction fermentation. The fermented (FT group) and unfermented (CT group) Astragalus solid powders were collected, respectively, and find out different metabolites and metabolic pathways through the sample pretreatment, LC-MS analysis and bioinformatics analysis. The result showed that the peak map of the total ion current map had good reproducibility. The main metabolites of fermented Astragalus had identified 183 metabolic components. The PCA map of the positive ion and negative ion mode samples could be distinguished well. The volcanic map analysis showed the metabolite changes were different between FT and CT groups, there were 1 416 metabolites up-regulated in positive ion model were enriched to 83 metabolic pathways, 935 metabolites down-regulated were enriched to 83 metabolic pathways;And 1 040 metabolites up-regulated in negative ion model were enriched to 52 metabolic pathways, 809 metabolites down-regulated were enriched to 45 metabolic pathways. The differential metabolites of fermented Astragalus acid, lipids, ketones and other amino acids were significantly increased, and olefins and other amino acids were significantly down-regulated, among them, 15 were up-regulated metabolites, and 2 were down-regulated metabolites. The key metabolites were mainly α-diethyl sulfate, 2-methyl citric acid, 3-isopropenyl-6-oxoheptanoic acid, etc., involving pyruvate metabolism, propionic acid metabolism, galactose metabolism and other pathways. The results provided a theoretical basis for the metabolites, fermentation interaction mechanism and clinical application of fermented Astragalus.

Key words: Lactobacillus plantarum; Astragalus; fermentation; LC-MS; metabolomics

中图分类号:

S816.6

乔宏兴, 张立恒, 张晓静, 宋予震, 边传周. 基于LC-MS代谢组学的植物乳杆菌发酵黄芪的代谢产物分析[J]. 中国畜牧兽医, 2021, 48(9): 3283-3292.

QIAO Hongxing, ZHANG Liheng, ZHANG Xiaojing, SONG Yuzhen, BIAN Chuanzhou. Metabonomics Analysis of Fermented Astragalus by Lactobacillus plantarum Based on LC-MS Metabonomics[J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(9): 3283-3292.

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