1株植物乳杆菌的生物学特性及体外抑菌活性研究

doi:10.16431/j.cnki.1671-7236.2023.02.024

中国畜牧兽医 ›› 2023, Vol. 50 ›› Issue (2): 666-673.doi: 10.16431/j.cnki.1671-7236.2023.02.024

1株植物乳杆菌的生物学特性及体外抑菌活性研究

尹杨燕^1,2, 潘艳³, 李小宁², 李军², 廖玉英², 李常挺², 韦天超¹, 彭昊²

1. 广西大学动物科学技术学院, 南宁 530004;
2. 广西壮族自治区兽医研究所, 广西兽医生物技术重点实验室, 南宁 530001;
3. 广西农业职业技术大学, 南宁 530007

收稿日期:2022-06-13 出版日期:2023-02-05 发布日期:2023-02-06
通讯作者: 韦天超, 彭昊 E-mail:hpeng2006@163.com
作者简介:尹杨燕,E-mail:1985717389@qq.com;潘艳,E-mail:panyan413@163.com
基金资助:
国家重点研发计划"广西大石山区优势特色产业提质增效技术集成与示范"(2021YFD1100100);广西重点研发计划(AB21238003);广西牛羊产业创新团队建设(nycytxgxcxtd-2021-09);国家水禽产业技术体系建设专项(CARS-42);广西肉鸡产业创新团队建设专项(nycytxgxcxtd);南宁市重点研发计划(20212023);防城港市重点研发计划(AB21014016);良庆区重点研发计划(202109);武鸣区重点研发(20210102)

Study on Biological Characteristics and Antibacterial Activity in vitro of a Lactobacillus plantarum Strain

YIN Yangyan^1,2, PAN Yan³, LI Xiaoning², LI Jun², LIAO Yuying², LI Changting², WEI Tianchao¹, PENG Hao²

1. Institute of Animal Science and Technology, Guangxi University, Nanning 530004, China;
2. Guangxi Key Laboratory of Veterinary Biotechnology, Guangxi Veterinary Research Institute, Nanning 530001, China;
3. Guangxi Agricultural Vocational University, Nanning 530007, China

Received:2022-06-13 Online:2023-02-05 Published:2023-02-06

摘要/Abstract

摘要： 【目的】试验旨在研究植物乳杆菌GX20200417-1菌株的生物学特性及体外抑菌活性,探讨其在生产中应用的可能性。【方法】将植物乳杆菌GX20200417-1菌株接种至不同pH和含不同浓度胆盐的PBS,以及人工胃肠液中,使用菌落计数分析其对酸、胆盐和人工胃肠液的耐受性。使用牛津杯法检测GX20200417-1菌株对大肠杆菌、金黄色葡萄球菌及沙门氏菌的抑菌能力;通过与霉菌毒素共培养后使用ELISA方法研究GX20200417-1菌株对霉菌毒素的降解能力,并饲喂小鼠进行安全性试验。【结果】植物乳杆菌GX20200417-1菌株能够耐受pH 2.0及0.3%胆盐,并在人工胃肠液中有至少1×10⁴ CFU/mL的活菌数;GX20200417-1菌株发酵上清液对大肠杆菌、沙门氏菌、金黄色葡萄球菌均有明显的抑制作用,对玉米赤霉素及黄曲霉毒素均有降解作用,但对呕吐毒素无降解作用;灌服GX20200417-1菌株后小鼠安全、无毒副作用。【结论】植物乳杆菌GX20200417-1菌株具有优良的益生特性,在畜禽生产中有一定的开发潜力。

关键词: 植物乳杆菌; GX20200417-1菌株; 生物学特性; 体外抑菌活性

Abstract: 【Objective】 The purpose of this study was to investigate the biological characteristics and antibacterial activity in vitro of a Lactobacillus plantarum GX20200417-1,and discuss the possibility of its application in production practice.【Method】 The GX20200417-1 strain was inoculated into PBS with different pH and different concentrations of bile salt,and artificial gastrointestinal juice,its tolerance to acid,bile salt and artificial gastrointestinal juice were analyzed by colony count. The antibacterial activities of Lactobacillus plantarum GX20200417-1 strain against Escherichia coli, Staphylococcus aureus and Salmonella were detected by Oxford cup method.The degradation ability of Lactobacillus plantarum to mycotoxin was studied by ELISA method after co-culture with mycotoxin.Finally,the mice were fed for safety test.【Result】 Lactobacillus plantarum GX20200417-1 strain could tolerate pH 2.0 and 0.3% bile salt,and had at least 1×10⁴CFU/mL viable bacteria in artificial gastrointestinal juice.The supernatant of Lactobacillus plantarum culture had obvious inhibitory effect on Escherichia coli, Salmonella and Staphylococcus aureus,it could degradation gibberellin and aflatoxin,but it has no effect on the degradation of vomiting toxin.The mice were safe without side effects after Lactobacillus plantarum GX20200417-1 strain gavage.【Conclusion】 Lactobacillus plantarum GX20200417-1 strain had excellent probiotic properties,and had certain development potential in livestock and poultry production.

Key words: Lactobacillus plantarum; GX20200417-1 strain; biological characteristics; antibacterial activityin vitro

中图分类号:

S816.3

尹杨燕, 潘艳, 李小宁, 李军, 廖玉英, 李常挺, 韦天超, 彭昊. 1株植物乳杆菌的生物学特性及体外抑菌活性研究[J]. 中国畜牧兽医, 2023, 50(2): 666-673.

YIN Yangyan, PAN Yan, LI Xiaoning, LI Jun, LIAO Yuying, LI Changting, WEI Tianchao, PENG Hao. Study on Biological Characteristics and Antibacterial Activity in vitro of a Lactobacillus plantarum Strain[J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(2): 666-673.

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1株植物乳杆菌的生物学特性及体外抑菌活性研究

Study on Biological Characteristics and Antibacterial Activity in vitro of a Lactobacillus plantarum Strain

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