1株鲤鱼源植物乳杆菌的分离鉴定及其生物学特性分析

doi:10.16431/j.cnki.1671-7236.2022.07.039

摘要/Abstract

摘要： 【目的】筛选具有优良益生特性的鲤源乳酸菌作为水产养殖用微生态制剂候选菌株。【方法】以鲤鱼肠道内容物及黏膜为菌株的分离来源，采用MRS培养基进行菌株分离纯化，经16S rRNA测序鉴定后，对分离菌株生长特性、产酸能力、耐酸、耐胆盐、抑菌特性、药物敏感性及安全性等生物学特性进行分析。【结果】试验成功分离到1株植物乳杆菌，将其命名为YY001，该分离株在MRS培养基上菌落形态为圆形、表面光滑、边缘整齐、呈乳白色，革兰氏染色为阳性、无芽孢、两端钝圆的短小杆菌；4 h后进入对数期生长，12 h后生长达到稳定期；具有较强的产酸能力，产酸曲线显示，0~12 h pH迅速下降，培养16 h的菌液pH达3.8；在pH 3.0和0.3%胆盐的条件下具有一定耐受性；该分离株对水产常见致病菌柱状黄杆菌、维气氏单胞菌和嗜水气单胞菌均具抑菌效果，且对柱状黄杆菌的抑菌效果最显著，抑菌圈直径达34.19 mm；药物敏感性试验结果显示，分离株对卡那霉素、链霉素和万古霉素耐药；每天一次连续1周灌胃10⁸CFU分离菌株对小鼠无毒害作用。【结论】本研究分离到的菌株YY001具有优良的生物学特性，可作为水产养殖用微生态制剂候选菌株，为后续制备鲤鱼用微生态制剂奠定基础。

关键词: 鲤鱼; 植物乳杆菌; 分离鉴定; 生物特性

Abstract: 【Objective】 The aim of this study was to screen the cyprin-derived lactic acid bacteria with excellent probiotics as candidate bacterial strains for aquaculture probiotics.【Method】 In this experiment,the intestinal contents and mucous membrane of carp were used as the sources of bacterial isolates.MRS medium was used for bacterial isolates and purification.After identification by 16S rRNA sequencing,the biological characteristics of isolated strains,such as growth characteristics,acid production capacity,acid tolerance,bile salt tolerance,antibacterial characteristics,drug sensitivity and safety were analyzed.【Result】 The results showed that a strain of Lactobacillus plantarum was successfully isolated and named YY001.On MRS medium,the colony morphology of the isolated strain was round,smooth surface,neat edge,milky white,Gram-positive,spun-free,blunt short-bacillus.The strain began to grow in logarithmic phase after 4 h,and reached a stable phase after 12 h.The strain had a strong acid production capacity.The acid production curve showed that pH decreased rapidly from 0 to 12 h,and pH reached 3.8 after 16 h culture.The strain had a certain tolerance to acid and bile salt at pH 3.0 and 0.3% bile salt.The strain had bacteriostatic effect on Flavobacterium columnar,Aeromonas vibrii and Aeromonas hydrophila,and the bacteriostatic effect on Flavobacterium columnar was very significant,and the diameter of bacteriostatic zone was 34.19 mm.The drug sensitivity test showed that the strain was resistant to kanamycin,streptomycin and vancomycin.Once a day for one week,10⁸ CFU isolated strain had no toxic effect on mice after feeding.【Conclusion】 The strain YY001 isolated in this study had excellent biological characteristics,which could be used as a candidate strain for aquaculture probiotics,and laid a foundation for the subsequent preparation of probiotics for carp.

Key words: carp; Lactobacillus plantarum; isolation and identification; biological characteristics

中图分类号:

S816.73

杨泽敏, 李双, 金正雨, 廖义潇, 杨颖, 文明. 1株鲤鱼源植物乳杆菌的分离鉴定及其生物学特性分析[J]. 中国畜牧兽医, 2022, 49(7): 2805-2811.

YANG Zemin, LI Shuang, JIN Zhengyu, LIAO Yixiao, YANG Ying, WEN Ming. Isolation,Identification and Biological Characteristics of a Strain of Lactobacillus plantarum from Carp[J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(7): 2805-2811.

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