丁酸梭菌对高胆碱饮食小鼠血浆游离脂肪酸组成的影响

doi:10.16431/j.cnki.1671-7236.2023.02.010

摘要/Abstract

摘要： 【目的】分析丁酸梭菌对高胆碱饮食造成脂代谢异常小鼠血浆游离脂肪酸组成(FFA)的影响,以阐明丁酸梭菌调节高胆碱膳食脂代谢的作用机制。【方法】选取4周龄健康的雄性昆明小鼠24只,随机分为3组:正常组、模型组和丁酸梭菌组,每组8只。高胆碱饮食造模8周后,给药7 d,禁食12 h,采集血浆、肝脏、附睾脂肪垫和肾周脂肪,对肝脏、脂肪称重,利用生化仪检测血脂水平;通过HE染色及油红O染色分别观察肝脏组织结构变化及脂滴沉积程度;利用液相色谱-质谱联用(LC-MS)技术检测小鼠血浆氧化三甲胺(TMAO)含量;利用气相色谱-质谱联用(GC-MS)技术及多元统计分析研究血浆中FFA组成。【结果】与模型组相比,丁酸梭菌组小鼠体重、脂肪增长得到显著抑制(P＜0.05),给予丁酸梭菌后小鼠肝脏脂肪沉积减少;丁酸梭菌显著或极显著降低了高胆碱饮食小鼠血浆中甘油三酯(TG)和低密度脂蛋白(LDL-C)含量(P＜0.05;P＜0.01),并使高密度脂蛋白(HDL-C)含量显著升高(P＜0.05);丁酸梭菌灌胃后,高胆碱饮食小鼠血浆TMAO浓度显著降低(P＜0.05);丁酸梭菌可显著降低高胆碱饮食小鼠血浆中棕榈酸(C16∶0)、硬脂酸(C18∶0)等饱和脂肪酸(SFA)含量,显著降低单不饱脂肪酸(MUFA)中棕榈烯酸(C16∶1)、油酸(C18∶1 N9c)含量,显著升高多不饱和脂肪酸(PUFA)中n-3型PUFA含量,其中二十二碳六烯酸(DHA)含量显著升高,n-6型PUFA中亚油酸(C18∶2 N6c)、γ-亚麻酸(C18∶3 N6)含量显著降低(P＜0.05)。此外,丁酸梭菌还显著降低了血浆中反油酸(C18∶1 N9t)、反亚油酸(C18∶2 N6t)含量(P＜0.05)。【结论】服用丁酸梭菌可调节高胆碱饮食小鼠游离脂肪酸组成及血浆TMAO含量,改善其脂代谢紊乱。

关键词: 丁酸梭菌; 高胆碱饮食; 游离脂肪酸(FFA); 氧化三甲胺(TMAO)

Abstract: 【Objective】 This study was aimed to analyze the effect of Clostridium butyricum on the composition of plasma free fatty acid (FFA) in mice with abnormal lipid metabolism caused by high choline diet,so as to clarify the mechanism of Clostridium butyricum regulating lipid metabolism in high choline diet.【Method】 24 healthy male Kunming mice aged 4 weeks were randomly divided into 3 groups:Normal,model and Clostridium butyricum groups,with 8 mice in each group.After 8 weeks of high choline diet,7 d of administration and 12 h of fasting,the plasma,liver,epididymal fat pad and perirenal fat were collected.Weighing the fat and liver,the blood lipid was detected by biochemical analyzer.HE staining and oil red O staining were used to observe the changes of liver tissue structure and the deposition degree of lipid droplets.The content of trimethylamine oxide (TMAO) in plasma was detected by liquid mass spectrometry (LC-MS) technology.The composition of FFA in plasma was studied by gas chromatography-mass spectrometry (GC-MS) technology and multivariate statistical analysis.【Result】 Compared with model group,the body weight and fat growth in Clostridium butyricum group were effectively inhibited (P＜0.05).After Clostridium butyrate administration,the deposition of liver lipid in mice was decreased. Clostridium butyrate significantly or extremely significantly decreased the contents of plasma triglyceride (TG) and low density lipoprotein (LDL-C) (P＜0.05 or P＜0.01),and significantly increased the content of high density lipoprotein (HDL-C) in mice fed with high choline diet (P＜0.05).After administration of Clostridium butyricum,the plasma TMAO concentration in mice fed with high choline diet was significantly decreased (P＜0.05).In Clostridium butyrate group,the contents of saturated fatty acids (SFA) such as palmitic acid (C16∶0) and stearic acid (C18∶0) were significantly decreased,the contents of palmitoleic acid (C16∶1) and oleic acid (C18∶1 N9C) in monounsaturated fatty acid (MUFA) were significantly decreased,the content of n-3 type polyunsaturated fatty acids (PUFA) was significantly increased,the content of docosahexaenoic acid (DHA) was significantly increased,and the contents of linoleic acid (C18∶2 N6C) and gamma linolenic acid (C18∶3 N6) in n-6 type PUFA were significantly decreased (P＜0.05).In addition, Clostridium butyrate significantly decreased the contents of trans isooleic acid (C18∶1 N9t) and trans linoleic acid (C18∶2 N6t) in plasma (P＜0.05).【Conclusion】 Clostridium butyrate could regulate FFA composition and plasma TMAO content in mice fed with high choline diet,and improve lipid metabolism disorder.

Key words: Clostridium butyricum; high choline diet; free fatty acid (FFA); trimethylamine oxide (TMAO)

中图分类号:

S852.61⁺6.3

陈静, 余术宜, 黄慧, 李健哲. 丁酸梭菌对高胆碱饮食小鼠血浆游离脂肪酸组成的影响[J]. 中国畜牧兽医, 2023, 50(2): 519-530.

CHEN Jing, YU Shuyi, HUANG Hui, LI Jianzhe. Effect of Clostridium butyrate on the Composition of Plasma Free Fatty Acid in Mice Fed with High Choline Diet[J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(2): 519-530.

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