丁酸梭菌-龙眼多糖发酵液对小鼠溃疡性结肠炎的防治作用

doi:10.16431/j.cnki.1671-7236.2020.11.037

中国畜牧兽医 ›› 2020, Vol. 47 ›› Issue (11): 3731-3738.doi: 10.16431/j.cnki.1671-7236.2020.11.037

丁酸梭菌-龙眼多糖发酵液对小鼠溃疡性结肠炎的防治作用

向蓉^1,2, 梁伟杰¹, 袁明贵¹, 彭新宇¹, 田雅¹, 魏琦麟¹, 周廷斤¹, 徐志宏^1,2

1. 广东省农业科学院动物卫生研究所, 农业农村部兽用药物与诊断技术广东科学观测实验站, 广东省畜禽疫病防治研究重点实验室, 广州 510640;
2. 岭南现代农业科学与技术广东省实验室肇庆分中心, 肇庆 526238

收稿日期:2020-06-01 出版日期:2020-11-20 发布日期:2020-11-20
通讯作者: 徐志宏 E-mail:zhihongxup@139.com
作者简介:向蓉(1981-),女,土家族,湖北来凤人,博士,副研究员,研究方向:兽药,E-mail:23753842@qq.com
基金资助:
广州市科技计划项目（201909020001、201604020138）

Preventive and Therapeutic Effects of Clostridium butyricum-longan Polysaccharide Fermentation Broth on Mouse Model with Ulcerative Colitis

XIANG Rong^1,2, LIANG Weijie¹, YUAN Minggui¹, PENG Xinyu¹, TIAN Ya¹, WEI Qilin¹, ZHOU Tingjin¹, XU Zhihong^1,2

1. Guangdong Provincial Key Laboratory of Animal Disease Prevention, Scientific Observing and Experimental Station of Veterinary Drugs and Diagnostic Techniques of Guangdong Province, Ministry of Agriculture and Rural Affairs, Institute of Animal Health, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China;
2. Zhaoqing Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Zhaoqing 526238, China

Received:2020-06-01 Online:2020-11-20 Published:2020-11-20

摘要/Abstract

摘要： 试验旨在观察丁酸梭菌-龙眼多糖发酵液预防给药对溃疡性结肠炎（ulcerative colitis，UC）模型小鼠的防治作用。30只SPF级雄性BALB/c小鼠随机分成空白组、模型组和发酵液组，10只/组。按10 mL/kg体重的剂量标准，空白组和模型组灌胃无菌去离子水，发酵液组灌胃丁酸梭菌-龙眼多糖发酵液，灌胃持续7 d。第8天开始，模型组和发酵液组连续7 d自由饮用5%葡聚糖硫酸钠（dextran sulfate sodium，DSS）溶液诱导UC模型，空白组自由饮用无菌水。试验第14天，称量所有小鼠体重，进行疾病活动指数（disease activity index，DAL）评分、临床评分，摘眼球采血后处死并解剖小鼠，取结肠肠段测量长度并进行组织学损伤评分，同时用甲醛固定后制作切片。ELISA检测血清中白细胞介素-6（interleukin-6，IL-6）、IL-10及肿瘤坏死因子-α（tumor necrosis factor-α，TNF-α）含量。结果表明，UC模型造模成功，模型组发病症状明显。与模型组相比，发酵液组小鼠体重下降程度显著降低（P<0.05），DAL评分、临床评分、结肠缩短程度及组织学损伤评分均极显著下降（P<0.01）。发酵液组小鼠结肠组织表现出相对完整的上皮层结构及隐窝，仅有部分炎症细胞侵入上皮组织。ELISA检测结果显示，发酵液组IL-6和TNF-α含量显著低于模型组（P<0.05），IL-10的含量显著高于模型组（P<0.05）。说明丁酸梭菌-龙眼多糖发酵液对DSS诱导的小鼠溃疡性结肠炎具有一定的防治作用，其作用机制可能与降低促炎因子IL-6和TNF-α含量、提升抗炎因子IL-10含量抑制炎症有关。

关键词: 丁酸梭菌; 龙眼多糖; 溃疡性结肠炎; 临床症状

Abstract: The aim of the study was to observe the preventive and therapeutic effect of Clostridium butyricum-longan polysaccharide fermentation broth on mouse model with ulcerative colitis (UC).30 SPF male BALB/c mice were randomly divided into blank group,model group and fermentation broth group,10 mice/group.According to the dosage standard of 10 mL/kg body weight,sterile deionized water was administered to the blank group and model group,and Clostridium butyricum-longan polysaccharide fermentation liquid was administered to the fermentation broth group for 7 days.From the 8th day,the model group and the fermentation broth group were allowed to drink 5% dextran sulfate sodium (DSS) solution for 7 consecutive days to induce the UC model,and the blank group was free to drink sterile water.On the 14th day,all mice were weighed,and DAL scores and clinical scores were scored.Then the mice were dissected after eyeball collection and blood sampling.The length of the colon was measured and scored for histological damage of the colon,sections were made after formalin fixation.The content of IL-6,IL-10 and TNF-α in serum were detected by ELISA.The results showed that UC model was established successfully and symptoms were obvious in the model group.Compared with the model group,the weight loss of the mice in the fermentation broth group was significantly reduced (P<0.05),DAL score,clinical score,colon shortening degree and histological injury score were extremely significantly reduced (P<0.01).The colon tissue of mice in the fermentation broth group showed a relatively complete epithelial structure and crypts,and only part of the inflammatory cells invaded the epithelial tissue.ELISA results showed that the IL-6 and TNF-α levels in the model group were significantly higher than those in the fermentation broth group (P<0.05),and the IL-10 content was significantly lower than that in the fermentation broth group (P<0.05).It showed that Clostridium butyricum-longan polysaccharide fermentation broth had a certain preventive effect on DSS-induced UC in mice,and its mechanism might be related to reducing the content of IL-6 and TNF-α,increasing the content of IL-10,and inhibiting inflammation.

Key words: Clostridium butyricum; longan polysaccharide; ulcerative colitis; clinical symptoms

中图分类号:

S856.4

向蓉, 梁伟杰, 袁明贵, 彭新宇, 田雅, 魏琦麟, 周廷斤, 徐志宏. 丁酸梭菌-龙眼多糖发酵液对小鼠溃疡性结肠炎的防治作用[J]. 中国畜牧兽医, 2020, 47(11): 3731-3738.

XIANG Rong, LIANG Weijie, YUAN Minggui, PENG Xinyu, TIAN Ya, WEI Qilin, ZHOU Tingjin, XU Zhihong. Preventive and Therapeutic Effects of Clostridium butyricum-longan Polysaccharide Fermentation Broth on Mouse Model with Ulcerative Colitis[J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(11): 3731-3738.

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丁酸梭菌-龙眼多糖发酵液对小鼠溃疡性结肠炎的防治作用

Preventive and Therapeutic Effects of Clostridium butyricum-longan Polysaccharide Fermentation Broth on Mouse Model with Ulcerative Colitis

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