小鼠溃疡性结肠炎模型的建立与评价

doi:10.16431/j.cnki.1671-7236.2016.01.025

›› 2016, Vol. 43 ›› Issue (1): 171-175.doi: 10.16431/j.cnki.1671-7236.2016.01.025

小鼠溃疡性结肠炎模型的建立与评价

曹明泽, 王旭荣, 王磊, 张景艳, 王海瑞, 李建喜, 王学智

中国农业科学院兰州畜牧与兽药研究所, 农业部兽用药物创制重点实验室, 甘肃省新兽药工程重点实验室, 兰州 730050

收稿日期:2015-07-29 出版日期:2016-01-20 发布日期:2016-01-23
通讯作者: 李建喜, 王学智 E-mail:lalala200011@163.com;wangxz628@sina.com
作者简介:曹明泽(1987-),女,河北石家庄人,硕士生,研究方向:临床兽医学,E-mail:danxiaosheep@163.com
基金资助:
防治螨病和痢疾藏中兽药制剂制备关键技术研究与应用(201303040-14);无抗藏兽药应用和疾病综合防控(201203008-2)

Establishment and Evaluation of Mouse Model of Ulcerative Colitis

CAO Ming-ze, WANG Xu-rong, WANG Lei, ZHANG Jing-yan, WANG Hai-rui, LI Jian-xi, WANG Xue-zhi

Key Laboratory of New Animal Drug Project of Gansu Province, Key Laboratory of Veterinary Pharmaceutics Discovery, Ministry of Agriculture, Lanzhou Institute of Husbandry and Pharmaceutical Science, Chinese Academy of Agricultural Sciences, Lanzhou 730050, China

Received:2015-07-29 Online:2016-01-20 Published:2016-01-23

摘要/Abstract

摘要： 本试验旨在通过筛选葡聚糖硫酸钠盐(DSS)最佳浓度建立小鼠溃疡性结肠炎模型。将30只BALB/c小鼠随机分为对照组、3.5% DSS组、5% DSS组,每组10只。小鼠自由饮水5 d,每天记录小鼠体重,观察粪便性状,测便潜血。试验结束后测血清TNF-α、结肠组织髓过氧化物酶(MPO)、丙二醛(MDA)、还原性谷胱甘肽(GSH)活性变化等指标,计算结肠重量/长度比值。与对照组相比,两试验组结肠组织中MPO活性显著升高(P<0.05),MDA含量显著升高(P<0.05),GSH含量显著降低(P<0.05)。结果表明,两种浓度葡聚糖硫酸钠盐均可造模成功,5% DSS组小鼠精神状态很差,表现嗜睡、萎靡状态,而3.5% DSS组小鼠精神状态良好,且组织中MPO、MDA、GSH与对照组相比均差异显著(P<0.05),与5% DSS组相比差异不显著(P>0.05),因此,选用浓度为3.5% DSS造模更合适。

关键词: 溃疡性结肠炎; 疾病活动指数; TNF-α; 髓过氧化物酶; 丙二醛; 还原型谷胱甘肽

Abstract: The experiment was aimed to establish mouse model of ulcerative colitis(uc) by screening the optimum concentration of dextran sulfate sodium salt(DSS).Thirty BALB/c mice were randomly assigned to control group, 3.5% DSS group and 5% DSS group, ten mice each group.Mice drank water freely for 5 days, the body weights of everyday were recorded, stool was observed and stool occult blood was tested.After the experiment, the changes of TNF-α, MPO, MDA and GSH were tested, and the colon weight/length ratio was calculated.Compared with control group, the activity of MPO and content of MDA in the experiment groups were significantly increased(P<0.05), and content of GSH was significantly decreased(P<0.05).3.5% and 5% DSS both could successfully establish mouse model of ulcerative colitis.Mice in 5% DSS group had poor mental state, such as lethargy, malaise;Mice in 3.5% DSS group were appropriate, the mice mental was good, MPO, MDA and GSH were significantly different compared with control group(P<0.05), but there were no difference compared with 5% DSS group(P>0.05).So 3.5% DSS was more appropriate than 5% DSS to establish mouse model of ulcerative colitis.

Key words: ulcerative colitis(UC); DAI; TNF-α; MPO; MDA; GSH

中图分类号:

R574.62

曹明泽, 王旭荣, 王磊, 张景艳, 王海瑞, 李建喜, 王学智. 小鼠溃疡性结肠炎模型的建立与评价[J]. , 2016, 43(1): 171-175.

CAO Ming-ze, WANG Xu-rong, WANG Lei, ZHANG Jing-yan, WANG Hai-rui, LI Jian-xi, WANG Xue-zhi. Establishment and Evaluation of Mouse Model of Ulcerative Colitis[J]. , 2016, 43(1): 171-175.

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小鼠溃疡性结肠炎模型的建立与评价

Establishment and Evaluation of Mouse Model of Ulcerative Colitis

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