基于溃疡性结肠炎模型探讨锦灯笼提取物的抗炎及抗氧化作用

doi:10.16431/j.cnki.1671-7236.2023.06.037

摘要/Abstract

摘要： 【目的】探讨锦灯笼提取物对葡聚糖硫酸钠（DSS）诱导的小鼠溃疡性结肠炎的治疗作用。【方法】将108只SPF级雄性昆明小鼠随机分为6组：空白对照组（CON）、模型对照组（DSS）、阳性对照组（SET）、锦灯笼提取物低（EPCF-L）、中（EPCF-M）、高剂量组（EPCF-H），每组18只。试验开始后，除CON组外的其余小鼠自由饮用3.0% DSS溶液，CON组小鼠正常饮水，不做任何特殊处理；从第8天起，SET组小鼠灌胃给予柳氮磺吡啶（100 mg/kg），EPCF-L、EPCF-M和EPCF-H组小鼠分别灌胃给予锦灯笼提取物0.25、0.5、1 g/kg，CON和DSS组小鼠以相同的方式给予蒸馏水，每日1次，每只0.2 mL，连续7 d。试验期间每日逐只称量小鼠体重，观察小鼠粪便情况，计算小鼠疾病活动指数（DAI）、结肠黏膜损伤指数（CMDI）及组织病理学变化，测定小鼠结肠组织超氧化物歧化酶（superoxide dismutase，SOD）、过氧化氢酶（catalase，CAT）、谷胱甘肽过氧化物酶（glutathione peroxidase，GSH-Px）及炎性因子白细胞介素-6（interleukin-6，IL-6）、IL-1β mRNA表达水平。【结果】与CON组相比，DSS组小鼠体重下降明显，粪便不成型甚至出现血便，DAI评分和CMDI评分显著升高（P<0.05），结肠组织可见大量炎性细胞，肠黏膜结构被破坏，SOD、CAT、GSH-Px活性显著降低（P<0.05），IL-6和IL-1β基因mRNA表达水平显著升高（P<0.05），表明小鼠溃疡性结肠炎模型建立成功。与DSS组相比，结肠组织中炎性细胞浸润、黏膜充血和出血等情况减少，SET、EPCF-M、EPCF-H组的DAI和CMDI评分显著降低（P<0.05），SOD、CAT、GSH-Px活性显著升高（P<0.05），IL-6和IL-1β基因mRNA表达水平显著降低（P<0.05）。【结论】锦灯笼提取物对DSS诱导的小鼠溃疡性结肠炎有治疗作用，其可能途径是提高机体的抗氧化功能及减少炎性因子的产生。

关键词: 锦灯笼提取物; 葡聚糖硫酸钠; 溃疡性结肠炎

Abstract: 【Objective】 The purpose of the experiment was to investigate the therapeutic effect of extracts of Physalis Calyx seu Fructus on dextran sodium sulfate (DSS)-induced ulcerative colitis in mouse.【Method】 108 SPF-grade male Kunming mice were randomly divided into 6 groups:Blank control (CON), model control (DSS), positive control (SET), and extracts of Physalis Calyx seu Fructus low (EPCF-L), medium (EPCF-M) and high dose (EPCF-H) groups, with 18 mice in each group.After the experiment started, all mice except those in CON group drank 3.0% DSS solution freely, while mice in CON group drank water normally without any special treatment, from day 8 onwards, mice in SET group were given sulfasalazine enteric-coated tablets (100 mg/kg) by gavage, and mice in EPCF-L, EPCF-M and EPCF-H groups were given gavage with 0.25, 0.5 and 1 g/kg extracts of Physalis Calyx seu Fructus respectively, and distilled water was given to mice in CON and DSS groups in the same way, once daily at 0.2 mL each, for 7 days.During the experiment, mice were weighed individually every day, the feces of mice were observed, the disease activity index (DAI), colonic mucosal injury index (CMDI) and histopathological changes of colon were calculated, and the antioxidant indexes of mouse colon tissues were determined including superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px), and inflammatory factors interleukin-6 (IL-6) and IL-1β mRNA expression levels in the colonic tissues of mice were measured.【Result】 Compared with CON group, the weight of mice in DSS group decreased significantly, and their feces did not form or even showed bloody stools. DAI score and CMDI score increased significantly (P<0.05).A large number of inflammatory cells were seen in the colon tissues, the intestinal mucosal structure was destroyed.The activities of SOD, CAT and GSH-Px were significantly reduced (P<0.05), and the mRNA expression levels of IL-6 and IL-1β gene were significantly increased (P<0.05), indicating that the mouse ulcerative colitis model was successfully established.Compared with DSS group, there was a reduction in inflammatory cell infiltration, mucosal congestion and haemorrhage in the colon tissue, DAI and CMDI scores were significantly reduced (P<0.05), activities of SOD, CAT and GSH-Px were significantly increased (P<0.05) and the mRNA expression levels of IL-6 and IL-1β gene in SET, EPCF-M and EPCF-H groups were significantly reduced (P<0.05).【Conclusion】 The therapeutic effect of Physalis Calyx seu Fructus extract on DSS-induced ulcerative colitis in mice might be achieved by improving the antioxidant function of the body and reducing the production of inflammatory factors.

Key words: extracts of Physalis Calyx seu Fructus; sodium dextran sulfate; ulcerative colitis

中图分类号:

S859.7

牛叶雨, 沈琪, 白勇, 孙盼盼, 孙娜, 尹伟, 范阔海, 李宏全, 孙耀贵. 基于溃疡性结肠炎模型探讨锦灯笼提取物的抗炎及抗氧化作用[J]. 中国畜牧兽医, 2023, 50(6): 2531-2539.

NIU Yeyu, SHEN Qi, BAI Yong, SUN Panpan, SUN Na, YIN Wei, FAN Kuohai, LI Hongquan, SUN Yaogui. Anti-inflammatory and Antioxidative Effects of Extracts of Physalis Calyx seu Fructus Based on Ulcerative Colitis Model[J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2531-2539.

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