螺旋藻激酶对大鼠动脉粥样硬化的影响

doi:10.16431/j.cnki.1671-7236.2019.12.037

摘要/Abstract

摘要： 本研究旨在观察螺旋藻激酶（SPK）对大鼠动脉粥样硬化（AS）的影响并探讨其可能的作用机制。取SD大鼠60只随机均分为正常对照组、模型组、阳性对照组（辛伐他汀，0.005 g/kg）和SPK低、中、高剂量组（80、160、320 U/kg）。除正常对照组外，其余各组通过高脂饲料建立大鼠AS模型，同时给予相应药物，每天1次，试验期12周。末次给药60 min后股动脉取血，分离血清，测定总胆固醇（TC）、高密度脂蛋白胆固醇（HDL-C）、低密度脂蛋白胆固醇（LDL-C）含量并计算动脉粥样硬化指数（AI）；测定大鼠血清丙二醛（MDA）、一氧化氮（NO）含量及超氧化物歧化酶（SOD）活性；通过苏木精-伊红（HE）染色法在光镜下观察腹主动脉血管内膜形态改变。结果显示，与正常对照组相比，模型组大鼠AI及血清MDA含量显著升高（P<0.05），血清NO含量和SOD活性显著下降（P<0.05）；与模型组比较，SPK高剂量大鼠AI和血清MDA含量显著降低（P<0.05），NO含量和SOD活性显著升高（P<0.05）；病理结果显示：模型组有明显粥样斑块形成，SPK各剂量组粥样斑块形成明显减少，尤其中、高剂量组与正常对照组无明显差异。综上所述：SPK有明显的预防AS的作用，其作用机制可能与降低MDA、提高NO含量和SOD活性有关。

关键词: 螺旋藻激酶(SPK); 大鼠; 动脉粥样硬化(AS); 丙二醛; 超氧化物歧化酶; 一氧化氮

Abstract: The study was aimed to observe the effects and preliminary mechanism of Spirulina kinase (SPK) on atherosclerosis (AS) model rats.60 SD rats were randomly divided into normal control group,model group,positive control group (simvastatin,0.005 g/kg),SPK low-dose,medium-dose and high-dose groups(80,160 and 320 U/kg).Except for normal control group,other groups were fed with high fat feed to establish the atherosclerosis model and were given relevant medicine intragastrically once a day for 12 weeks.The artery blood was collected and the content of total cholesterol (TC),high density lipoprotein cholesterol (HDL-C),low density lipoprotein cholesterol (LDL-C) levels,contents of MDA,NO and SOD activity were measured,the atherosclerosis index (AI) was caculated.The change of thoracic aorta were observed by hematoxylin and eosin (HE) staining under microscope.The results showed that:Compared with normal control group,AI and MDA content were significantly increased (P<0.05),SOD activity and NO level were significantly decreased(P<0.05) in model group;Compared with the model group,MDA content was significantly decreased (P<0.05),while SOD activity and NO level were significantly increased (P<0.05) in SPK high-dose group.Pathological results showed that the model group had obvious atherosclerotic plaque formation.The formation of atherosclerotic plaque in SPK groups was significantly reduced,especially in the middle and high dose groups,there was no obvious difference comparing to the normal control group.In conclusion,SPK had obvious effect of preventing AS.Its mechanism might be related to the decrease of MDA and the increase of SOD activity and NO level.

Key words: Spirulina kinase (SPK); rats; atherosclerosis (AS); malondialdehyde; superoxide dismutase; nitric oxide

中图分类号:

R965

王慧杰, 庞辉, 韦娇, 伍果. 螺旋藻激酶对大鼠动脉粥样硬化的影响[J]. 中国畜牧兽医, 2019, 46(12): 3768-3774.

WANG Huijie, PANG Hui, WEI Jiao, WU Guo. Effects of Spirulina Kinase on Atherosclerosis Model Rats[J]. China Animal Husbandry and Veterinary Medicine, 2019, 46(12): 3768-3774.

参考文献

[1] 刘俊田.动脉粥样硬化发病的炎症机制和研究进展[J].西安交通大学学报(医学版),2015,26(52):141-152. LU J T.Progress in inflammatory pathogenesis of atherosclerosis[J].Journal of Xi'an Jiaotong University (Medical Edition),2015,26(52):141-152.(in Chinese)
[2] 吴杰.螺旋藻的营养保健价值及在预防医学中的应用探究[J].中国卫生产业,2017,14(15):18-19. WU J.Nutritional health value of Spirulina and its application in preventive medicine[J].China Health Industry,2017,14(15):18-19.(in Chinese)
[3] 何善生,王力,李健,等.螺旋藻研究进展[J].食品工业,2017,12:263-268. HE S S,WANG L,LI J,et al.Progress in Spirulina research[J].Food Industry,2017,12:263-268.(in Chinese)
[4] 张文,吴清平,吴军林.螺旋藻营养保健价值及开发应用进展[J].食品与发酵科技,2013,49(3):89-92. ZHANG W,WU Q P,WU J L.The nutrition health value and research progress of Spirulina[J].Food and Fermentation Technology,2013,49(3):89-92.(in Chinese)
[5] 肖云晓,陈高斯,庞辉,等.螺旋藻激酶分离提纯方法初探[J].时珍国医国药,2011,22(6):1399-1400. XIAO Y X,CHEN G S,PANG H,et al.Separation and purification methods of Spirulina kinase purification method[J].Lishizhen Medicine and Materia Medica Research,2011,22(6):1399-1400.(in Chinese)
[6] 庞辉,蒋皓,李小花,等.螺旋藻蛋白激酶溶栓作用的研究[J].时珍国医国药,2012,23(10):2521-2522. PANG H,JIANG H,LI X H,et al.Effects of Spirulina kinase on thrombolysis[J].Lishizhen Medicine and Materia Medica Research,2012,23(10):2521-2522.(in Chinese)
[7] 赵杰,肖云晓,蒋皓,等.螺旋藻激酶对人脐静脉内皮细胞t-PA、mRNA、PAI-1、mRNA表达的影响[J].时珍国医国药,2012,23(6):1471-1473. ZHAO J,XIAO Y X,JIANG H,et al.Effects of Spirulina kinase on the expression of t-PA,mRNA,PAI-1,mRNA in human umbilical vein endothelial cell[J].Lishizhen Medicine and Materia Medica Research,2012,23(6):1471-1473.(in Chinese)
[8] 赵杰,庞辉,李想,等.螺旋藻激酶对大鼠内皮损伤模型纤溶酶原和抗凝血酶Ⅲ的影响[J].中国老年学杂志,2017,37(6):1462-1464. ZHAO J,PANG H,LI X,et al.Effects of Spirulina kinase on Plg and antithrombin Ⅲ in endothelial damage thrombosis model[J].Chinese Journal of Gerontology,2017,37(6):1462-1464.(in Chinese)
[9] 王慧杰,肖云晓,庞辉,等.螺旋藻激酶对肾上腺素损伤内皮细胞分泌t-PA和PAI-1的影响[J].基因组学与应用生物学,2016,35(5):1049-1053. WANG H J,XIAO Y X,PANG H,et al.Effect of Spirulina kinase on drenaline injured t-PA and PAI-1 expression in human umbilical vein endothelial cells[J].Genomics and Applied Biology,2016,35(5):1049-1053.(in Chinese)
[10] 何秋璟,王慧杰,庞辉,等.螺旋藻激酶对动脉粥样硬化模型大鼠血管内皮功能的影响[J].中国药房,2017,19:2628-2631. HE Q J,WANG H J,PANG H,et al.Effects of Spirulina kinase on vascular endothelial function of model rats with atherosclerosis[J].China Pharmacy,2017,19:2628-2631.(in Chinese)
[11] 陈萌,庞辉,王慧杰,等.螺旋藻激酶对人脐静脉内皮细胞抗氧化能力的影响[J].中国药理学通报,2016,2:293-294. CHEN M,PANG H,WANG H J,et al.Effect of Spirulina kinase on HUVEC induced by peroxide[J].Chinese Pharmacological Bulletin,2016,2:293-294.(in Chinese)
[12] 周红,吴晓燕,袁艺标,等.三种剂量维生素D₃结合高脂饲料建立大鼠动脉粥样硬化模型的比较[J].中国动脉硬化杂志,2012,11:995-998. ZHOU H,WU X Y,YUAN Y B,et al.Comparison of methods for establishing a rat model of atherosclerosis using three-doses of vitamin D₃ and atherogenic diet[J].China Journal of Arterioscler,2012,11:995-998.(in Chinese)
[13] 李波,李雄英,吕圭源,等.复方虎杖提取物对高脂血症模型大鼠血脂水平和动脉硬化指数的影响[J].中药新药与临床药理,2014,25(3):260-263. LI B,LI X Y,LYU G Y,et al.Effect of compound Rhizoma polygoni cuspidati extract on blood lipid levels and atherogenic index in hyperlipidemia model rats[J].Traditional Chinese Drug Research & Clinical Pharmacology,2014,25(3):260-263.(in Chinese)
[14] 彭璇,薛立群,张居作,等.PPARγ对猪血管内皮细胞增殖、迁移及小管形成的影响[J].中国畜牧兽医,2017,44(9):2809-2815. PENG X,XUE L Q,ZHANG J Z,et al.Effect of PPARγ on proliferation,migration and tube formation of vein endothelial cells[J].China Animal Husbandry & and Veterinary Medicine,2017,44(9):2809-2815.(in Chinese)
[15] SHRESTHA S,IRVIN M R,GRUNFELD C,et al.HIV,inflammation,and calcium in atherosclerosis[J].Arteriosclerosis,Thrombosis and Vascular Biology,2014,34(2):244-250.
[16] CHEN C,JIANG J,LU J M,et al. Resistin decreases expression of endothelial nitric oxide synthase through oxidative stress in human coronary artery endothelial cells[J].American Journal of Physiology,Heart and Circulatory Physiology,2010,299(1):H193-H201.
[17] LASSEGUE B,SAN MARTIN A,GRIENDLING K K.Biochemistry,physiology,and pathophysiology of NADPH oxidases in the cardiovascular system[J].Circulation Research,2012,110(10):1364-1390.
[18] EL ASSAR M,ANGULO J,RODRIGUEZ-MANAS L.Oxidative stress and vascular inflammation in aging[J].Free Radical Biology Medicine,2013,65:380-401.
[19] WEBER C,NOELS H. Atherosclerosis:Current pathogenesis and therapeutic options[J].Nature Medicine,2011,17(11):1410-1422.
[20] CAVIERES V,VALDES K,MORENO B,et al.Vascular hypercontractility and endothelial dysfunction before development of atherosclerosis in moderate dyslipidemia:Role for nitric oxide and interleukin-6[J].The American Journal of Cardiovascular Disease,2014,4(3):114-122.
[21] KOZLOV S G,KHAMCHIEVA L S,POGORELOVA O A,et al.Dynamics of asymptomatic atherosclerosis of carotid arteries depending on the achieved level of cholesterol in moderate-risk patients[J].Angiology and Vascular Surgery,2018,24(2):11-18.