板蓝根超微粉对大鼠免疫功能调节作用的研究

doi:10.16431/j.cnki.1671-7236.2019.07.034

摘要/Abstract

摘要：

为研究板蓝根经超微粉碎后对大鼠免疫功能调节的作用影响，利用环磷酰胺构建大鼠免疫低下模型，将80只SD大鼠随机分为4.组，即模型组、普通粉组、超微粉组及空白对照组，每组20只。40 mg/kg环磷酰胺腹腔注射5.d制造免疫抑制大鼠模型，第6.天开始给药，除空白组、模型组灌服生理盐水外，其他组给药5 g/kg。第7.、14天采集血样及大鼠肝脏、脾脏、胸腺组织，测定免疫器官指数、白细胞数量、C3b受体花环形成率（C3b-R）、红细胞免疫复合物花环形成率（ICR）、吞噬百分率、吞噬指数等指标，实时荧光定量PCR检测肿瘤坏死因子α（TNF-α）、白细胞介素2（IL-2） mRNA转录水平，Western blotting法检测TNF-ɑ和IL-2蛋白表达水平。结果显示，模型组大鼠白细胞数量、体重、胸腺和脾脏免疫器官指数较空白对照组均显著降低（P<0.05）。第14天时，超微粉组巨噬细胞吞噬百分率达到54.75%，吞噬指数为0.58；C3b-R为9.44%，显著高于普通粉组（8.97%）（P<0.05），超微粉组ICR为6.09%，低于普通粉组（6.33%）（P>0.05）。与普通粉组相比，第14天板蓝根超微粉组肝脏中TNF-α、IL-2 mRNA转录水平和蛋白水平的表达提高。综上所述，板蓝根超微粉和普通粉均可显著增强大鼠非特异性免疫功能，但超微粉效果优于普通粉。

关键词: 板蓝根超微粉; 板蓝根普通粉; SD大鼠; 免疫调节

Abstract:

In order to study the effects of ultrafine powder of Radix Isatidis on immune regulation in rats,cyclophosphamide was used to construct the model of immunodeficiency.80 SD rats were randomly divided into 4 groups:Model group,ordinary powder group,ultrafine powder group and blank control group with 20 rats in each group.40 mg/kg cyclophosphamide was injected intraperitoneally for 5 d.From 6th day,The blank control group and model group were given normal saline,and the other groups were given 5 g/kg ordinary powder or ultrafine powder.At 7th and 14th day,the blood samples and liver,spleen and thymus tissues were collected.The white blood cell count (WBC),organ indexes of spleen and thymus,C3b-R,ICR,phagocytosis indexes were detected.Real-time PCR was used to detect the transcription levels of TNF-α and IL-2 mRNA.The expression of TNF-ɑ and IL-2 protein were detected by Western blotting.The results showed that the WBC,body weight,thymus and spleen indexes in model group were significantly lower than those in the blank control group (P<0.05).On the 14th day,the percentage of macrophage phagocytosis of rat in ultrafine powder group reached 54.7%,the index of macrophage phagocytosis was 0.58.The C3b-R of rat at 14th day in ultrafine powder group was 9.44%,significantly higher than that of the ordinary power group (8.97%)(P<0.05).And the ICR of ultrafine powder group was 6.09%,lower than that of the ordinary power group (6.33%)(P>0.05).Compared with the ordinary powder group,the ultrafine powder of Radix Isatidis could increase the expression of TNF-ɑ and IL-2 in liver on the 14th day.In conlusion,ordinary powder and ultrafine powder of Radix Isatidis could both enhance the nonspecific immune function of rats,and ultrafine powder was superior to the ordinary powder.

Key words: Radix Isatidis ultrafine powder; Radixi Iatidis ordinary powder; SD rat; immunoregulation

中图分类号:

R283

姜晓文, 王雪微, 于文会, 张欣, 侯耀杰, 郝敬友. 板蓝根超微粉对大鼠免疫功能调节作用的研究[J]. 《中国畜牧兽医》, 2019, 46(7): 2159-2166.

JIANG Xiaowen, WANG Xuewei, YU Wenhui, ZHANG Xin, HOU Yaojie, HAO Jingyou. Study on Immunoregulation of Radix Isatidis Ultrafine Powder in Rats[J]. , 2019, 46(7): 2159-2166.

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