恩诺沙星与磺胺二甲嘧啶联合毒性探究

doi:10.16431/j.cnki.1671-7236.2021.10.040

摘要/Abstract

摘要： 本研究旨在探究恩诺沙星与磺胺二甲嘧啶的联合毒性。选取SD大鼠为试验对象，将其分成6组，每组12只，分别为高剂量联合用药组（500 mg/kg体重）、中剂量联合用药组（250 mg/kg体重）、低剂量联合用药组（50 mg/kg体重）、恩诺沙星单药组（250 mg/kg体重）、磺胺二甲嘧啶单药组（250 mg/kg体重）及对照组（等量0.5%羧甲基纤维素钠溶液），配制相应的药物进行灌胃，最后一次给药1 d后对大鼠进行称重、麻醉、心脏采血并剖检取肝脏组织，然后对大鼠增重率、血常规指标、血清生化指标及肝脏病理学变化几个方面进行分析，并利用SPSS 22.0软件评估2种药物的联合作用效果。结果显示，在雌性大鼠中，高、中剂量联合用药组使大鼠增重率显著下降（P<0.05），雄性大鼠增重率无显著变化（P>0.05）；雄性大鼠给药组白细胞数量均显著增加（P<0.05），高剂量联合用药组中性粒细胞和淋巴细胞占比分别表现为显著升高及下降（P<0.05）；高、中剂量联合用药组大鼠外周血中谷草转氨酶含量显著增加（P<0.05），且高、中剂量联合用药组肝脏病理切片视野可见不同程度的炎性细胞浸润，并伴有不同程度的损伤。本研究结果表明，恩诺沙星和磺胺二甲嘧啶联用可使毒性增加，对大鼠的免疫系统状态有一定的影响，且能造成一定程度的肝脏损伤，剂量越高影响越大。本研究为恩诺沙星和磺胺二甲嘧啶联合用药机制的研究提供数据支持，并为二者的临床应用提供参考，提示新的食品安全评估应考虑药物联合暴露带来的影响。

关键词: 恩诺沙星; 磺胺二甲氧嘧啶; SD大鼠; 联合毒性

Abstract: The aim of this study was to evaluate the combined toxicity of enrofloxacin and sulfamethazine. SD rats were selected and divided into 6 groups:High dose group (500 mg/(kg·BW)), middle dose group (250 mg/(kg·BW)), low dose group (50 mg/(kg·BW)), enrofloxacin group (250 mg/(kg·BW)), sulfamethazine group (250 mg/(kg·BW)) and control group 0.5% CMC, each group contained 12 rats. The corresponding drugs were prepared for intragastric administration. 1 d after the last administration, rats were weighed, heart blood was collected and liver tissues were dissected. Then the weight gain rate, blood routine indicators, serum biochemical indicators and liver pathological changes of the rats were analyzed, and the combined effect of the two drugs was evaluated by SPSS 22.0 software. The results showed that, in female rats, a significant decline of the weight gain rate was found at high and middle dose groups (P<0.05), but there was no significant change in male rats (P>0.05). Leukocyte populations in male rats had a significant rise (P<0.05), neutrophil and lymphocyte ratios were significantly increased and decreased at high dose group, respectively (P<0.05). The content of peripheral blood AST of male rats was significantly increased at high and middle dose groups (P<0.05), and liver pathologic reports revealed different degrees of inflammatory cell infiltration with different degrees of injury at high and middle dose groups. This study showed that mixing enrofloxacin and sulfamethazine might increase toxicity, it had a certain effect on the rat immune system, and could cause liver damage, and the higher the dose, the greater the effect. This work provided data support for the joint toxicity mechanism of enrofloxacin and sulfamethazine, and provided references for their clinical application. In addition, it suggested that new food safety assessment should consider the effects caused by co-exposure to multiple drugs.

Key words: enrofloxacin; sulfamethazine; SD rats; combined toxicity

中图分类号:

S859.79⁺6

赵军杰, 陈可心, 栾业辉, 程林丽. 恩诺沙星与磺胺二甲嘧啶联合毒性探究[J]. 中国畜牧兽医, 2021, 48(10): 3872-3879.

ZHAO Junjie, CHEN Kexin, LUAN Yehui, CHENG Linli. Evaluation of Combined Toxicity of Enrofloxacin and Sulfamethazine[J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(10): 3872-3879.

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