牛磺酸复合添加剂对免疫低下小鼠免疫功能的影响

doi:10.16431/j.cnki.1671-7236.2022.12.013

摘要/Abstract

摘要： 【目的】研究在基础饲粮中添加牛磺酸复合添加剂对免疫低下模型小鼠免疫功能的影响，旨在为提高动物免疫机能的营养调控措施提供一定的理论依据。【方法】将80只小鼠随机均分为4组：C和M组小鼠饲喂基础饲粮，H和MH组小鼠给予含有8.8%的牛磺酸复合添加剂的基础饲粮，试验期51 d。M和MH组小鼠于试验第31天起连续4 d腹腔注射70 μg/g BW环磷酰胺（CTX），制备免疫低下模型，其余小鼠腹腔注射等体积生理盐水。统计整个试验期的日采食量，每周称量小鼠体重，并计算平均日采食量（ADFI）和平均日增重（ADG）。试验结束后，采集小鼠血清及胸腺和脾脏，检测小鼠免疫器官指数、细胞免疫、体液免疫、非特异性免疫功能及血清中细胞因子和免疫球蛋白水平的变化。【结果】与C组相比，M组小鼠ADFI、耳肿胀度、血清溶血素水平、免疫球蛋白G （IgG）水平显著降低（P<0.05），ADG、胸腺指数、脾淋巴细胞增殖能力、抗体生成细胞量、腹腔巨噬细胞吞噬能力、白介素6（IL-6）和免疫球蛋白A （IgA）水平极显著降低（P<0.01），脾脏指数极显著升高（P<0.01）；碳廓清指数和免疫球蛋白M （IgM）水平无显著差异（P>0.05）；与M组相比，MH组小鼠的ADFI、ADG、胸腺指数、脾淋巴细胞增殖能力、耳肿胀度和IgM水平均显著提高（P<0.05），血清溶血素水平、抗体生成细胞量、腹腔巨噬细胞吞噬能力、IL-6和IgA水平均极显著提高（P<0.01），脾脏指数显著降低（P<0.05），碳廓清指数和IgG水平无显著差异（P>0.05）。【结论】该牛磺酸复合添加剂能够改善由CTX所致免疫低下小鼠的固有免疫和适应免疫，改善细胞因子和免疫球蛋白的水平，起到增强小鼠免疫力的作用。

关键词: 牛磺酸复合添加剂; 环磷酰胺; 小鼠; 免疫调节

Abstract: 【Objective】 This experiment was conducted to study the effect of adding taurine additive package in basic diet on the immune function of immunosuppressed mice.【Method】 80 mice were randomly and equally divided into four groups, mice in C and M groups were given basic diet, mice in H and MH groups were given diet containing 8.8% taurine additive package.The experiment was lasted for 51 days, the immunosuppressed mice model was established by intraperitoneal injection of 70 μg/g BW cyclophosphamide (CTX) for four consecutive days from the 31st day (in M and MH groups), while the other mice were intraperitoneally injected with the equal volume of normal saline.The feed intake and body weight were recorded to calculate the average daily gain (ADG) and average daily feed intake (ADFI).Effects of this immunopotentiator on immune organ index, cellular immunity, humoral immunity, non-specific immune function, levels of cytokines and immunoglobulins of mice were detected.【Result】 Compared with C group, mice in M group showed significant reduction in ADFI, ear swelling, serum hemolysin level, and immunoglobulin G(IgG) (P<0.05), while ADG, thymus index, splenic lymphocyte proliferation capacity, antibody-producing cell amount, abdominal macrophage phagocytosis, interleukin-6(IL-6) and immunoglobulin A(IgA) levels were extremely significantly reduced (P<0.01), and the spleen index was extremely significantly increased (P<0.01).There were no significant differences in carbon clearance index and immunoglobulin M(IgM) level between C and M groups (P>0.05).Compared with M group, mice in MH group had significantly increased ADFI, ADG, thymus index, spleen lymphocyte proliferation, ear swelling and IgM levels (P<0.05), extremely significantly increased serum hemolysin level, antibody-producing cell amount, abdominal macrophage phagocytosis, IL-6 and IgA levels (P<0.01), and significantly decreased spleen index (P<0.05).There was no significant difference in carbon clearance index and IgG levels between M and MH group (P>0.05).【Conclusion】 The taurine additive package administered in the present study could improve the innate and adaptive immunity, improve the levels of cytokines and immunoglobulins of mice with low immunity caused by CTX, and play a role in enhancing the immunity of mice.

Key words: taurine additive package; cyclophosphamide; mice; immunoregulation

中图分类号:

S816.7

郭子萌, 王福正, 王承志, 杜鹏, 张明月, 李泽垚, 苏晓晨, 吴高峰, 杨建成. 牛磺酸复合添加剂对免疫低下小鼠免疫功能的影响[J]. 中国畜牧兽医, 2022, 49(12): 4635-4645.

GUO Zimeng, WANG Fuzheng, WANG Chengzhi, DU Peng, ZHANG Mingyue, LI Zeyao, SU Xiaochen, WU Gaofeng, YANG Jiancheng. Effect of Taurine Additive Package on the Immune Function of Immunosuppressed Mice[J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(12): 4635-4645.

参考文献

[1] DING S J, JIANG H M, FANG J.Regulation of immune function by polyphenols[J].Journal of Immunology Research, 2018, 2018:1-8.
[2] FU R Q, LIANG C, CHEN D W, et al.Effects of dietary Bacillus coagulans and yeast hydrolysate supplementation on growth performance, immune response and intestinal barrier function in weaned piglets[J].Journal of Animal Physiology and Animal Nutrition, 2021, 105(5):898-907.
[3] CHEN C Y, CHEN J S, WANG S Q, et al.Effects of different dietary ratios of linolenic to linoleic acids or docosahexaenoic to eicosapentaenoic acids on the growth and immune indices in grouper, epinephelus coioides[J].Aquaculture, 2017, 473:153-160.
[4] GONG Y L, YANG F, HU J P, et al.Effects of dietary yeast hydrolysate on the growth, antioxidant response, immune response and disease resistance of largemouth bass (Micropterus salmoides)[J].Fish Shellfish Immunology, 2019, 94:548-557.
[5] WANG L, ZHAO N, ZHANG F, et al.Effect of taurine on leucocyte function[J].European Journal of Pharmacology, 2009, 616(1-3):275-280.
[6] EL IDRISSI A.Taurine regulation of neuroendocrine function[J].Advances in Experimental Medicine and Biology, 2019, 1155:977-985.
[7] 梁肖娜, 叶馨阳, 孔彦文, 等.牛肝中牛磺酸对免疫抑制小鼠免疫及抗氧化能力的影响[J].肉类研究, 2016, 30(11):1-5. LIANG X N, YE X Y, KONG Y W, et al.Effect of taurine from bovine liveron immune functions and antioxidant capacity in immunosuppressed mice[J].Meat Reserch, 2016, 30(11):1-5.(in Chinese)
[8] CARDOSO C, AFONSO C, BANDARRA N M.Dietary DHA and health:Cognitive function ageing[J].Nutrition Research Reviews, 2016, 29(2):281-294.
[9] GUTIERREZ S, SVAHN S L, JOHANSSON M E.Effects of omega-3 fatty acids on immune cells[J].International Journal of Molecular Sciences, 2019, 20(20):5028.
[10] 韩丽荣, 魏硕名, 王旭锋, 等.二十二碳六烯酸免疫调节活性[J].食品科学, 2018, 39(3):206-212. HAN L R, WEI S M, WANG X F, et al.Immunomodulatory activity of docosahexaenoic acid[J].Food Science, 2018, 39(3):206-212.(in Chinese)
[11] 贺淼, 黄鑫, 陈中平, 等.酵母水解物的消化吸收及营养作用[J].中国饲料, 2014, 509(9):38-41. HE M, HUANG X, CHEN Z P, et al.The digestion and absorption of yeast hydrolyzate and its nutritional value[J].China Feed, 2014, 509(9):38-41.(in Chinese)
[12] 徐芳.银杏叶提取物的体外抑菌及免疫调节作用研究[D].长沙:湖南农业大学, 2014. XU F.Investigation on the in vitro antibacterial activity and immune modulatory function of Ginkgo biloba extract[D].Changsha:Hunan Agricultural University, 2014.(in Chinese)
[13] 田俊娜, 王婷婷, 王双, 等.黄芪增益方对小鼠免疫功能的影响[J].中南药学, 2021, 19(8):1613-1618. TIAN J N, WANG T T, WANG S, et al.Immunomodulatory effect of Huangqi Zengyi formula on mice[J].Central South Pharmacy, 2021, 19(8):1613-1618.(in Chinese)
[14] 丁明雪, 刘庆静, 邹积宏.香菇多糖联合肠内营养剂对免疫低下小鼠的影响[J].生物技术, 2022, 32(1):79-84. DING M X, LIU Q J, ZOU J H.Effect of lentinan combined with enteral nutrition on immunocompromised mice[J].Biotechnology, 2022, 32(1):79-84.(in Chinese)
[15] 赵庆枫.蛋虫草培养物免疫功能调节作用的研究与应用[D].长春:吉林大学, 2019. ZHAO Q F.Research and application of immune function regulation of egg cordyceps militaris culture[D].Changchun:Jilin University, 2019.(in Chinese)
[16] 唐耿秋, 严寒静, 倪石寿, 等.铁皮石斛口含片、泡腾片对小鼠免疫功能影响的研究[J].时珍国医国药, 2020, 31(2):324-326. TANG G Q, YAN H J, NI S S, et al.Studies on the effect of Dendrobium and effervescent tablets on the immune function of mice[J].Lishizhen Medicine and Materia Medica Research, 2020, 31(2):324-326.(in Chinese)
[17] 张兆肖, 郑铮, 杨丽, 等.骆驼初乳对环磷酰胺诱导小鼠免疫功能低下的调节作用[J].现代食品科技, 2019, 35(4):1-8. ZHANG Z X, ZHENG Z, YANG L, et al.Effects of camel colostrum on cyclophosphamide-induced immunodeficiency in mice[J].Modern Food Science and Technology, 2019, 35(4):1-8.(in Chinese)
[18] 刘燕, 王雪, 史玉柱, 等.滨蒿提取物对环磷酰胺致小鼠免疫抑制的调节作用[J].免疫学杂志, 2022, 38(4):354-360. LIU Y, WANG X, SHI Y Z, et al.Immunomodulatory effects of Artemisia scoparia extract on cyclophosphamide-induced immunosuppression in mice[J].Immunological Journal, 2022, 38(4):354-360.(in Chinese)
[19] 冯学轩, 王弋, 严家荣, 等.天然蛋白与酵母β-葡聚糖组合物增强免疫力功能研究[J].中国免疫学杂志, 2021, 37(16):1938-1942. FENG X X, WANG Y, YAN J R, et al.Study on immunity enhancement of natural protein and yeast-β-glucan combination[J].Chinese Journal of Immunology, 2021, 37(16):1938-1942.(in Chinese)
[20] 樊乃境, 王冬梅, 高悦, 等.山药蛋白肽对免疫能力低下小鼠的免疫调节作用[J].食品与发酵工业, 2020, 46(6):101-107. FAN N J, WANG D M, GAO Y, et al.Immunomodulatory effects of the iron yam peptides on mice with immunocompromised[J].Food and Fermentation Industries, 2020, 46(6):101-107.(in Chinese)
[21] 庞艳华, 郝福星, 韩玲玲, 等.茶树油提取物粉对小鼠免疫功能的影响[J].中国兽药杂志, 2019, 53(3):72-78. PANG Y H, HAO F X, HAN L L, et al.Effects of the powder of tea tree oil extract on the immune function of mice[J].Chinese Journal of Veterinary Drug, 2019, 53(3):72-78.(in Chinese)
[22] 邓桦, 杨鸿, 张兆, 等.玉屏风复合多糖对环磷酰胺免疫抑制小鼠的恢复作用[J].中国兽医学报, 2014, 34(2):288-292. DENG H, YANG H, ZHANG Z, et al.Immune restorative effect of YPF-P on immunosuppressive mice influenced by cyclophosphamide[J].Chinese Journal of Veterinary Science, 2014, 34(2):288-292.(in Chinese)
[23] PIAO J, MENG F P, FANG H, et al.Effect of taurine on thymus differentiation of DEX-induced immunosuppressive mice[J].Advances in Experimental Medicine and Biology, 2019, 1155:381-390.
[24] 刘奇, 慎凯峰, 周丹英, 等.当归口服液对正常小鼠免疫功能的影响[J].海峡药学, 2020, 32(12):19-23. LIU Q, SHEN K F, ZHOU D Y, et al.Effect of Angelica oral liquid on immune function of normal mice[J].Strait Pharmaceutical Journal, 2020, 32(12):19-23.(in Chinese)
[25] 刘东方, 陆军, 陆煜, 等.鼠李糖乳杆菌LV108及其发酵乳免疫调节作用的比较[J].中国微生态学杂志, 2020, 32(12):1365-1369. LIU D F, LU J, LU Y, et al.The immunomodulatory effects of Lactobacillus rhamnousus LV108 and its fermented milk[J].Chinese Journal of Microecology, 2020, 32(12):1365-1369.(in Chinese)
[26] 赵明明, 范秋领.牛磺酸营养液对小鼠免疫功能影响的研究[J].食品与药品, 2011, 13(3):97-99. ZHAO M M, FAN Q L.Effect of taurine nutrient solution on immune function of immunosuppressive mice[J].Food and Drug, 2011, 13(3):97-99.(in Chinese)
[27] FENG C L, LI L Y, LI Q, et al.Docosahexaenoic acid ameliorates autoimmune inflammation by activating GPR120 signaling pathway in dendritic cells[J].International Immunopharmacology, 2021, 97:107698.
[28] 薛云浩, 李娜娜, 王雯颍.DHA藻油鳕鱼肝油软胶囊对小鼠免疫调节功能的影响[J].中国卫生检验杂志, 2018, 28(12):1416-1418. XUE Y H, LI N N, WANG W Y.Effect of DHA algal oil and cod liver oil capsule on immunoregulatory function in mice[J].Chinese Journal of Health Laboratory, 2018, 28(12):1416-1418.(in Chinese)
[29] 马琴.二十二碳六烯酸-磷脂生物活性的研究[D].青岛:中国海洋大学, 2009. MA Q.Studies on the bioactivities of docosahexaenoic acid-phosphatidylcholine[D].Qingdao:Ocean University of China, 2009.(in Chinese)
[30] 黄明亮.南瓜叶乙醇提取物对小鼠免疫功能的影响[D].重庆:西南大学, 2013. HUANG M L.Effects of ethanol extract of pumpkin (Cucurbita moschata) leaves on immune function in mice[D].Chongqing:Southwest University, 2013.(in Chinese)
[31] 宋万里.牛磺酸对B淋巴细胞的活化作用及其机理研究[D].大连:大连医科大学, 2019. SONG W L.Activation mechanism of taurine to the B lymphocyte[D].Dalian:Dalian Medical University, 2019.(in Chinese)
[32] 张秀珍, 杜卫, 沈芳兰, 等.牛磺酸对小鼠免疫功能的影响[J].青岛医学院学报, 1996, 1:40-41. ZHANG X Z, DU W, SHEN F L, et al.The effects of taurine on mouse immune functions[J].Journal of Qingdao University(Medicine Science), 1996, 1:40-41.(in Chinese)
[33] 马琴, 王静凤, 王琦, 等.二十二碳六烯酸-磷脂脂质体对S₁₈₀荷瘤小鼠肿瘤抑制和免疫调节作用[J].中国药科大学学报, 2008, 2:159-163. MA Q, WANG J F, WANG Q, et al.Anti-tumor activities and immune regulation of docosahexaenoic acid-phosphatidylcholine liposomes in mice bearing S₁₈₀[J].Journal of China Pharmaceutical University, 2008, 2:159-163.(in Chinese)
[34] 姚壮民, 陈琼, 王万里, 等.DHA藻油脂质体对小鼠免疫功能调节作用研究[J].食品安全质量检测学报, 2021, 12(15):6200-6207. YAO Z M, CHEN Q, WANG W L, et al.Effect of DHA algae oil liposomes on mouse immune function[J].Journal of Food Safety & Quality, 2021, 12(15):6200-6207.(in Chinese)
[35] 郭亚惠, 刘志勇, 陈丽玲, 等.不同取浆时间的蜂王浆对小鼠免疫功能的影响[J].食品与生物技术学报, 2016, 35(8):828-833. GUO Y H, LIU Z Y, CHEN L L, et al.Effects of royal jellies with different harvesting time on the immune functions of mice[J].Journal of Food Science and Biotechnology, 2016, 35(8):828-833.(in Chinese)
[36] 颉志刚, 凌洁彬, 鲁纪刚, 等.牛磺酸对虎纹蛙非特异性免疫及血清氧化还原状态的影响[J].动物学杂志, 2017, 52(6):996-1002. XIE Z G, LING J B, LU J G, et al.Effects of taurine on non-specific immunity and serum redox state in hoplobatrachus rugulosus[J].Chinese Journal of Zoology, 2017, 52(6):996-1002.(in Chinese)
[37] 郑昱, 荆云.牛磺酸增强免疫力和抗疲劳功能试验研究[J].人人健康, 2016, 433(20):248. ZHENG Y, JING Y.Experimental study on enhance immunity and anti-fatigue function of taurine[J].Health for Everyone, 2016, 433(20):248.(in Chinese)
[38] DONG J, CHENG R J, YANG Y Y, et al.Effects of dietary taurine on growth, non-specific immunity, anti-oxidative properties and gut immunity in the Chinese mitten crab Eriocheir sinensis[J].Fish & Shellfish Immunology, 2018, 82:212-219.
[39] 殷成港, 李习龙, 蒋显仁.酵母水解物的生物学功能及其在猪生产中的应用[J].动物营养学报, 2022, 34(6):3435-3442. YIN C G, LI X L, JIANG X R, et al.Biological functions of yeast hydrolysate and its application in pig production[J].Chinese Journal of Animal Nutrition, 2022, 34(6):3435-3442.(in Chinese)
[40] 徐军发, 侯敢, 黄迪南, 等.酵母多糖对小鼠腹腔巨噬细胞产生一氧化氮和白细胞介素-1的影响[J].中国生化药物杂志, 2002, 2:67-68. XU J F, HOU G, HUANG D N, et al.Effects of zymosan on the production of nitric oxide and interleukin-1 in mouse peritoneal macrophages[J].Chinese Journal of Biochemical and Pharmaceuticals, 2002, 2:67-68.(in Chinese)
[41] 张海全, 黄勤英, 郑广进, 等.罗汉果多糖对环磷酰胺所致的免疫抑制小鼠免疫功能的影响[J].广西植物, 2019, 39(11):1573-1582. ZHANG H Q, HUANG Q Y, ZHENG G J, et al.Effects of Siraitia grosvenorii polysaccharides on immune function in immunosuppressed mice induced by cyclophosphamide[J].Guihaia, 2019, 39(11):1573-1582.(in Chinese)
[42] 李春亭, 赵欣, 闫可心, 等.豆王酵素对小鼠胃肠运动和免疫功能的影响[J].延边大学农学学报, 2021, 43(3):61-66. LI C T, ZHAO X, YAN K X, et al.Effects of Douwang enzyme on gastrointestinal motility and immune function in mice[J].Journal of Agricultural Science Yanbian University, 2021, 43(3):61-66.(in Chinese)
[43] SONG C J, GAN S Q, HE J, et al.Effects of nano-zinc on immune function in Qianbei-pockmarked goats[J].Biological Trace Element Research, 2021, 199(2):578-584.
[44] 马启伟, 郭梁, 刘波, 等.牛磺酸对卵形鲳鲹肠道微生物及免疫功能的影响[J].南方水产科学, 2021, 17(2):87-96. MA Q W, GUO L, LIU B, et al.Effect of taurine intestinal microbes and immune function in golden pompano(Trachinotus ovatus)[J].South China Fisheries Science, 2021, 17(2):87-96.(in Chinese)