冷应激复温对ICR小鼠神经行为学的影响

doi:10.16431/j.cnki.1671-7236.2023.03.009

摘要/Abstract

摘要： 【目的】研究不同冷应激复温后对ICR小鼠的自发运动、探索行为、焦虑情绪和血液激素指标的影响。【方法】通过与28 ℃常温对照组相比,检测ICR小鼠4、10、16、22 ℃冷应激复温组于复温2、4、6、8、10、12 h后在旷场试验和高架十字迷宫试验中的运动变化,并通过ELISA检测各组在复温2及4 h后的血液激素指标变化。【结果】旷场试验结果显示,与28 ℃常温对照组相比,22 ℃冷刺激复温10 h时中央区域停留时间和运动时间显著减少(P＜0.05);16 ℃冷刺激复温4和8 h时中央区域停留时间显著减少(P＜0.05);10 ℃冷刺激复温4和10 h时中央区域停留时间显著减少(P＜0.05),复温2 h中央区域运动距离显著增加(P＜0.05);4 ℃冷刺激复温6 h时中央区域停留时间极显著减少(P＜0.01),复温2 h中央区域运动距离极显著增加(P＜0.01)。高架十字迷宫试验结果显示,与28 ℃常温对照组相比,22和4 ℃冷刺激复温4 h、16 ℃冷刺激复温10 h闭臂停留时间显著或极显著下降(P＜0.05; P＜0.01);10 ℃冷刺激复温组闭臂停留时间无显著变化(P＞0.05)。与28 ℃常温对照组相比,皮质酮(CORT)在22 ℃冷刺激复温4 h时极显著升高(P＜0.01),16 ℃冷刺激复温2和4 h时极显著升高(P＜0.01);肾上腺素(EPI)在22 ℃冷刺激复温4 h时极显著升高(P＜0.01),10 ℃冷刺激复温2和4 h时极显著升高(P＜0.01)。【结论】冷应激结束后对ICR小鼠的自发运动、探索行为、焦虑情绪和血液激素指标存在持续影响,应减少以ICR小鼠为模型的试验环境温度变化,提高试验结果准确性。

关键词: ICR小鼠; 冷应激; 复温; 行为学; 皮质酮(CORT); 肾上腺素(EPI)

Abstract: 【Objective】 The aim of this study was to investigate the effects of spontaneous movement,exploratory behavior,anxiety and blood hormone indexes in ICR mice with different cold stress rewarming.【Method】 Compared with 28 ℃ normal temperature control group,the movement changes of ICR mice in the open field test and elevated plus maze test after rewarming 2,4,6,8,10 and 12 h in 4,10,16 and 22 ℃ cold stress rewarming group were detected,respectively,and the changes of blood hormone indexes in each group after rewarming 2 and 4 h were detected by ELISA.【Result】 The results of open field test showed that compared with 28 ℃ normal temperature control group,the residence time and exercise time of central region were significantly reduced after 10 h of rewarming at 22 ℃ cold stimulation (P＜0.05).The residence time in the central region was significantly decreased after 4 and 8 h of rewarming at 16 ℃ cold stimulation (P＜0.05).The residence time in the central region was significantly decreased after 4 and 10 h of rewarming at 10 ℃ cold stimulation (P＜0.05),and the movement distance of the central region was significantly increased after 2 h of rewarming (P＜0.05).The residence time of the central region was extremely significantly decreased after 6 h of rewarming at 4 ℃ cold stimulation (P＜0.01),and the movement distance of the central region was extremely significantly increased after 2 h of rewarming (P＜0.01).Elevated plus maze test showed that compared with 28 ℃ normal temperature control group,the residence time of closed arms was significantly or extremely significantly decreased after 4 h of rewarming at 22 and 4 ℃ cold stimulation and 10 h of rewarming at 16 ℃ cold stimulation (P＜0.05 or P＜0.01).There was no significant change in the residence time of closed arm in 10 ℃ cold stimulation rewarming group (P＞0.05).Corticosterone (CORT) was extremely significantly increased after 4 h of rewarming at 22 ℃ cold stimulation (P＜0.01),and was extremely significantly increased after 2 and 4 h of rewarming at 16 ℃ cold stimulation (P＜0.01).Epinephrine (EPI) was extremely significantly increased after 4 h of rewarming at 22 ℃ cold stimulation (P＜0.01),and extremely significantly increased after 2 and 4 h of rewarming at 10 ℃cold stimulation (P＜0.01).【Conclusion】 After the end of cold stress,the spontaneous movement,exploration behavior,anxiety and blood hormone indexes of ICR mice would continue to be affected.It was necessary to reduce the temperature change of the test environment with ICR mice as the model and improve the accuracy of the test results.

Key words: ICR mice; cold stress; rewarming; roethology; corticosterone (CORT); epinephrine (EPI)

中图分类号:

S852.2

史宏昭, 刘洋, 姚睿智, 刘鹏, 李士泽. 冷应激复温对ICR小鼠神经行为学的影响[J]. 中国畜牧兽医, 2023, 50(3): 932-941.

SHI Hongzhao, LIU Yang, YAO Ruizhi, LIU Peng, LI Shize. Effects of Cold Stress Rewarming on Neurobehavioral Changes in ICR Mice[J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(3): 932-941.

参考文献

[1] 刘洋,姚睿智,史宏昭,等.ICR小鼠自发运动、探索行为和焦虑情绪的检测及其参考值的确立[J].黑龙江八一农垦大学学报,2020,32(1):29-35. LIU Y,YAO R Z,SHI H Z,et al.Detection of spontaneous movement,exploration behavior and anxiety in ICR mice and establishment of reference range of corresponding indicators[J]. Journal of Heilongjiang Bayi Agricultural University,2020,32(1):29-35.(in Chinese)
[2] MALONEY S K,FULLER A,MITCHELL D,et al.Translating animal model research:Does it matter that our rodents are cold?[J].Physiology (Bethesda,Md),2014,29(6):413-420.
[3] MARTIN B,JI S,MAUDSLEY S,et al."Control" laboratory rodents are metabolically morbid:Why it matters[J].Proceedings of the National Academy of Sciences of the United States of America,2010,107(14):6127-6133.
[4] RATUSKI A S,WEARY D M.Environmental enrichment for rats and mice housed in laboratories:A metareview[J].Animals,2022,12(4):414.
[5] CAIT J,CAIT A,SCOTT R W,et al.Conventional laboratory housing increases morbidity and mortality in research rodents:Results of a meta-analysis[J].BMC Biology,2022,20(1):15.
[6] GORDON C J.The mouse thermoregulatory system:Its impact on translating biomedical data to humans[J].Physiology & Behavior,2017,179:55-66.
[7] MESSMER M N,KOKOLUS K M,ENG J W,et al.Mild cold-stress depresses immune responses:Implications for cancer models involving laboratory mice[J].BioEssays,2014,36(9):884-891.
[8] NABENISHI H,YAMAZAKI A.Impaired reproduction in Japanese Black cattle under cold environmental conditions[J].Reproduction in Domestic Animals,2017,52(3):371-375.
[9] MORIGNAT E,GAY E,VINARD J L,et al.Impact of heat and cold waves on female cattle mortality beyond the effect of extreme temperatures[J].Journal of Thermal Biology,2018,78:374-380.
[10] XU T,LI X,YANG L,et al.Impact of cold exposure on the reproductive function in female rats[J].BioMed Research International,2018,2018:3674906.
[11] VIALARD F,OLIVIER M.Thermoneutrality and immunity:How does cold stress affect disease?[J].Frontiers in Immunology,2020,11:588387.
[12] 童卓云.冷刺激与高脂饮食对大鼠精子功能的影响及其生殖生物学基础的研究[D].乌鲁木齐:新疆医科大学,2020. TONG Z Y.Effects of cold stimulation and high-fat diet on sperm function in rats and its reproductive biology[D].Urumqi:Xinjiang Medical University,2020.(in Chinese)
[13] MIFSUD K R,REUL J.Mineralocorticoid and glucocorticoid receptor-mediated control of genomic responses to stress in the brain[J].Stress (Amsterdam,Netherlands),2018,21(5):389-402.
[14] KETCHESIN K D,STINNETT G S,SEASHOLTZ A F.Corticotropin-releasing hormone-binding protein and stress:From invertebrates to humans[J].Stress (Amsterdam,Netherlands),2017,20(5):449-464.
[15] LECORPS B,WEARY D M,VON KEYSERLINGK M A G.Captivity-induced depression in animals[J].Trends in Cognitive Sciences,2021,25(7):539-541.
[16] GODOY L D,ROSSIGNOLI M T,DELFINO-PEREIRA P,et al.A comprehensive overview on stress neurobiology:Basic concepts and clinical implications[J].Frontiers in Behavioral Neuroscience,2018,12:127.
[17] COUNCIL N R.Guide for the Care and Use of Laboratory Animals[M].Washington (DC):National Academies Press,2011.
[18] KOKOLUS K M,CAPITANO M L,LEE C T,et al.Baseline tumor growth and immune control in laboratory mice are significantly influenced by subthermoneutral housing temperature[J].Proceedings of the National Academy of Sciences of the United States of America,2013,110(50):20176-20181.
[19] GASKILL B N,ROHR S A,PAJOR E A,et al.Working with what you’ve got:Changes in thermal preference and behavior in mice with or without nesting material[J].Journal of Thermal Biology,2011,36(3):193-199.
[20] GASKILL B N,ROHR S A,PAJOR E A,et al.Some like it hot:Mouse temperature preferences in laboratory housing[J].Applied Animal Behaviour Science,2009,116(2-4):279-285.
[21] CANNON B,NEDERGAARD J.Nonshivering thermogenesis and its adequate measurement in metabolic studies[J].The Journal of Experimental Biology,2011,214(Pt 2):242-253.
[22] YIALLOURIS A,TSIOUTIS C,AGAPIDAKI E,et al.Adrenal aging and its implications on stress responsiveness in humans[J].Frontiers in Endocrinology,2019,10:54.
[23] 刘嘉莉,窦金焕,胡丽蓉,等.热应激对奶牛生理和免疫功能的影响及其机理[J].中国畜牧兽医,2018,45(1):263-270. LIU J L,DOU J H,HU L R,et al.The effect of heat stress on the physiological and immune functions of dairy cows and its mechanism[J] China Animal Husbandry & Veterinary Medicine,2018,45(1):263-270.(in Chinese)
[24] 李留安,彭峰,杨凤.游泳应激对小鼠肾脏、肺脏和脑组织脂质过氧化状态的影响[J].中国畜牧兽医,2012,39(10):159-162. LI L A,PENG F,YANG F.Effects of swimming stress on lipid peroxidation in kidneys,lungs and brain tissues in mice[J] China Animal Husbandry & Veterinary Medicine,2012,39(10):159-162.(in Chinese)
[25] SINGLETON J M,GARLAND T J R.Influence of corticosterone on growth,home-cage activity,wheel running,and aerobic capacity in house mice selectively bred for high voluntary wheel-running behavior[J]. Physiology & Behavior,2019,198:27-41.
[26] UNDERWOOD G E,BAKER C A,WEED S D.Protective effect of elevated temperature on mice infected with Coe virus[J].Journal of Immunology (Baltimore,Md:1950),1966,96(6):1006-1012.
[27] MCKIE G L,SHAMSHOUM H,HUNT K L,et al.Intermittent cold exposure improves glucose homeostasis despite exacerbating diet-induced obesity in mice housed at thermoneutrality[J].The Journal of Physiology,2022,600(4):829-845.
[28] 王亚男,冯曼,周英昊,等.冀北寒区秋冬季节荷斯坦奶牛产奶性能及血液常规指标的变化规律研究[J].中国畜牧兽医,2020,47(4):1282-1288. WANG Y N,FENG M,ZHOU Y H,et al.Study on the change law of milk production traits and routine blood indicators of Holstein dairy cows in the North of Hebei province in autunm and winter[J] China Animal Husbandry & Veterinary Medicine,2020,47(4):1282-1288.(in Chinese)
[29] 姜文杰,李英花,姚雪瑞,等.冷、热应激对猪孤雌胚胎体外发育的影响[J].中国畜牧兽医,2018,45(9):2507-2515. JIANG W J,LI Y H,YAO X R,et al.Effects of cold or heat stress on the development of porcine parthenogenetic embryos in vitro[J] China Animal Husbandry & Veterinary Medicine,2018,45(9):2507-2515.(in Chinese)
[30] DEVALLANCE E,RIGGS D,JACKSON B,et al.Effect of chronic stress on running wheel activity in mice[J]. PLoS One,2017,12(9):e0184829.
[31] VON ZIEGLER L M,FLORIOU-SERVOU A,WAAG R,et al.Multiomic profiling of the acute stress response in the mouse hippocampus[J].Nature Communications,2022,13(1):1824.
[32] OLIVIER K,REINDERS L A,CLARKE M W,et al.Maternal,placental,and fetal responses to intermittent heat exposure during late gestation in mice[J]. Reproductive Sciences (Thousand Oaks, Calif),2021,28(2):416-425.
[33] BADACHE S,BOUSLAMA S,BRAHMIA O,et al.Prenatal noise and restraint stress interact to alter exploratory behavior and balance in juvenile rats,and mixed stress reverses these effects[J].Stress (Amsterdam,Netherlands),2017,20(3):320-328.
[34] NAKAGAWA H,MATSUNAGA D,ISHIWATA T.Effect of heat acclimation on anxiety-like behavior of rats in an open field[J].Journal of Thermal Biology,2020,87:102458.
[35] TSENG H H,WATTS J J,KIANG M,et al.Nigral stress-induced dopamine release in clinical high risk and antipsychotic-naḯve schizophrenia[J].Schizophrenia Bulletin,2018,44(3):542-551.
[36] PIJLMAN F T,VAN REE J M.Physical but not emotional stress induces a delay in behavioural coping responses in rats[J].Behavioural Brain Research,2002,136(2):365-373.
[37] PIJLMAN F T,HERREMANS A H,VAN DE KIEFT J,et al.Behavioural changes after different stress paradigms:Prepulse inhibition increased after physical,but not emotional stress[J].European Neuropsychopharmacology,2003,13(5):369-380.
[38] 刘洋.冷刺激及复温对小鼠相关神经行为学的影响[D].大庆:黑龙江八一农垦大学,2019. LIU Y.Effects of cold stimulation and rewarming on related neurobehavioral in mice[D].Daqing:Heilongjiang Bayi Agricultural University,2019.(in Chinese)
[39] CHEN X,BOLLINGER E,CUNIO T,et al.An assessment of thermoneutral housing conditions on murine cardiometabolic function[J].American Journal of Physiology Heart and Circulatory Physiology,2022,322(2):H234-H245.
[40] BACQUÉ-CAZENAVE J,BHARATIYA R,BARRIÈRE G,et al.Serotonin in animal cognition and behavior[J].International Tournal of Molecular Sciences,2020,21(5):1649.