桃金娘果多糖对免疫抑制小鼠免疫功能的影响

doi:10.16431/j.cnki.1671-7236.2022.02.035

摘要/Abstract

摘要： 【目的】探讨桃金娘果多糖对免疫抑制小鼠免疫功能的影响。【方法】腹腔注射环磷酰胺80 μg/g BW, 1次/d, 连续3 d, 建立小鼠免疫抑制模型。将造模成功的小鼠随机分为5组(每组16只): 模型组、黄芪多糖组、桃金娘果多糖低、中、高剂量组。另设16只健康小鼠为正常组。黄芪多糖组每只小鼠按200 μg/g BW剂量腹腔注射黄芪多糖混悬液, 桃金娘果多糖低、中、高剂量组分别按50、100、200 μg/g BW剂量腹腔注射桃金娘果多糖混悬液, 正常组和模型组每只小鼠腹腔注射等体积的生理盐水, 1次/d, 连续7 d。试验结束后, 分别测定小鼠脏器指数(胸腺、脾脏)、血清中白细胞介素1β(IL-1β)、白细胞介素2(IL-2)、白细胞介素6(IL-6)、γ干扰素(IFN-γ)、溶菌酶(LZM)的含量和过氧化物酶(POD)活性。【结果】桃金娘果多糖不同剂量组小鼠胸腺指数和脾脏指数均有不同程度提高, 尤以桃金娘果中剂量组最高, 与模型组相比差异极显著(P<0.01)。桃金娘果多糖不同剂量组IL-6、IL-1β血清细胞因子含量均极显著高于模型组(P<0.01);桃金娘果多糖低、中剂量组的血清IFN-γ、IL-2含量相较于模型组都有所提升, 但差异均不显著(P>0.05), 桃金娘果多糖高剂量组的IFN-γ、IL-2含量均极显著高于模型组(P<0.01)。桃金娘果多糖不同剂量组小鼠的POD活性和LZM含量均极显著或显著高于模型组(P<0.01;P < 0.05)。【结论】桃金娘果多糖中剂量即100 μg/g BW对免疫抑制小鼠免疫功能的恢复效果最佳。

关键词: 桃金娘果多糖; 环磷酰胺; 免疫调节; 细胞因子

Abstract: 【Objective】 This study was aimed to investigate the effect of Rhodomyrtus tomentosa fruit polysaccharide on immunological function in immunosuppressed mice.【Method】 Cyclophosphamide was injected intraperitoneally with 80 μg/g BW, once a day, for 3 consecutive days, and mice immunosuppressive model was established.The successfully modeled mice were divided into 5 groups randomly (16 in each group): model group, astragalus polysaccharide group and Rhodomyrtus tomentosa fruit polysaccharide low, medium and high dose group.Another 16 healthy mice were set as normal group.Each mouse in astragalus polysaccharide group was intraperitoneally injected with astragalus polysaccharide suspension at a dose of 200 μg/g BW, and in Rhodomyrtus tomentosa fruit polysaccharide low, medium, and high-dose groups was treated with 50, 100 and 200 μg/g BW Rhodomyrtus tomentosa fruit polysaccharide suspension, respectively.The mice in normal group and model group were treated with normal saline of equal volume.This injection was administered once a day for 7 days.After the test, thymus index, spleen index, the content of IL-1β, IL-2, IL-6, IFN-γ, LZM and POD activity in serum were determined.【Result】 The thymus index and spleen index were increased by different doses of Rhodomyrtus tomentosa fruit polysaccharide, and the differences between the medium dose group and model group were extremely significant (P < 0.01).The contents of IL-6 and IL-1β in different doses of Rhodomyrtus tomentosa fruit polysaccharide group were extremely significantly higher than those in model group (P < 0.01).Compared with model group, the IFN-γ and IL-2 levels in low and medium dose groups increased, but the difference was not significant (P > 0.05).The contents of IFN-γ and IL-2 in high-dose group were extremely significantly higher than those in the model group (P < 0.01).The POD activity and LZM content of mice in different doses of Rhodomyrtus tomentosa fruit polysaccharide groups were extremely significantly or significantly higher than those in the model group (P < 0.01;P < 0.05).【Conclusion】The medium dose of Rhodomyrtus tomentosa fruit polysaccharide (100 μg/g BW) had the best effect on the recovery of immune function in immunosuppressive mice.

Key words: Rhodomyrtus tomentosa fruit polysaccharide; cyclophosphamide; immunomodulatory; cytokines

中图分类号:

S853.74

银慧慧, 童艳梅, 曾雪颜, 卢光强, 赵武, 姜源明, 刘伟. 桃金娘果多糖对免疫抑制小鼠免疫功能的影响[J]. 中国畜牧兽医, 2022, 49(2): 731-737.

YIN Huihui, TONG Yanmei, ZENG Xueyan, LU Guangqiang, ZHAO Wu, JIANG Yuanming, LIU Wei. Effect of Polysaccharide from Rhodomyrtus tomentosa Fruit on Immunological Function in Immunosuppressed Mice[J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(2): 731-737.

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