西藏那曲市羊源大肠杆菌分离鉴定及耐药性研究

doi:10.16431/j.cnki.1671-7236.2019.12.036

摘要/Abstract

摘要： 为了解西藏那曲市羊大肠杆菌的耐药情况，指导临床进行合理用药，本试验从那曲市采集羊新鲜无污染腹泻物92份，进行大肠杆菌显色培养基分离、革兰氏染色镜检、生化鉴定、分子生物学鉴定、致泻性大肠杆菌生化鉴定、药敏试验及耐药基因检测。结果显示，分离菌株在大肠杆菌显色培养基上呈蓝色菌落、革兰氏染色为粉红色的短杆菌，通过生化鉴定及23S rRNA的PCR检测得到26株羊源大肠杆菌，分离率为28.3%；其中25株符合致泻性大肠杆菌生化特性，致泻菌株分离率为27.2%。药敏试验结果显示，所得25株羊源大肠杆菌对氨苄西林的耐药性较高，耐药率为24.0%；对羧苄西林、卡那霉素的耐药性次之，耐药率为8%；对哌拉西林、头孢呋辛、庆大霉素、四环素、米诺霉素等药物耐药率为4%；对诺氟沙星、氧氟沙星、环丙沙星等药物极为敏感，可作为临床用药。5种耐药基因检测结果显示，bla_TEM基因检出率为100%，表明分离菌均含有相应的耐药基因。以上结果表明，西藏那曲市羊源大肠杆菌对多种药物耐药，提示在临床实践过程中应注重合理用药、联合用药，减缓大肠杆菌耐药性的产生。

关键词: 羊; 大肠杆菌; 分离鉴定; 药敏试验; 耐药基因

Abstract: In order to understand the drug resistance of Escherichia coli (E.coli) in sheep in Naqu city,Tibet,and to guide clinical rational drug use,92 fresh non-polluting diarrhea samples were collected from sheep in Naqu city,Tibet,and were separated by colour medium of E.coli,examined by Gram staining microscopy,biochemical identification,molecular biological identification and biochemical identification of diarrhogenic E.coli,drug sensitivity test and drug resistance gene detection.The results showed that the isolates were blue colony and pink brevibacterium on the colour medium of E.coli.26 strains of E.coli from sheep were isolated by biochemical identification and PCR detection of 23S rRNA,the isolation rate was 28.3%,25 strains of which accorded with the biochemical characteristics of diarrhogenic E.coli,and the isolation rate of diarrhogenic E.coli was 27.2%.Drug susceptibility test showed that 25 strains of sheep-derived E.coli were resistant to ampicillin with a resistance rate of 24.0%,followed by carboxybenicillin and kanamycin with a resistance rate of 8%,piperacillin,cefuroxime,gentamicin,tetracycline and minocycline with a resistance rate of 4%.Drugs such as norfloxacin,oxygen,floxacin and ciprofloxacin were very sensitive and could be used as clinical drugs.The detection rate of bla_TEM gene was 100% in 5 drug-resistant genes,which indicated that all isolates contained corresponding drug-resistant genes.The above results showed that there was resistance to many drugs in E.coli isolated from sheep in Naqu,Tibet,suggesting that rational drug use and combined drug use should be emphasized in clinical practice to slow down the emergence of drug resistance of E.coli.

Key words: sheep; E.coli; isolation and identification; drug susceptibility test; drug resistance gene

中图分类号:

S852.61

陈家露, 蔡重振, 次旦, 高家登, 扎西次仁, 索朗斯珠, 罗润波. 西藏那曲市羊源大肠杆菌分离鉴定及耐药性研究[J]. 中国畜牧兽医, 2019, 46(12): 3759-3767.

CHEN Jialu, CAI Chongzhen, Cidan, GAO Jiadeng, Zhaxiciren, Suolangsizhu, LUO Runbo. Isolation,Identification and Drug Resistance Analysis of Sheep Escherichia coli in Naqu,Tibet[J]. China Animal Husbandry and Veterinary Medicine, 2019, 46(12): 3759-3767.

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