穿王消炎粉对LPS诱导大鼠急性肺损伤的治疗作用

doi:10.16431/j.cnki.1671-7236.2022.04.039

摘要/Abstract

摘要： 【目的】研究穿王消炎粉对脂多糖(LPS)诱导大鼠急性肺损伤(ALI)的治疗作用。【方法】将60只SD雄性大鼠随机分入空白组、左氧氟沙星阳性药物对照组、LPS模型组及穿王消炎粉低(1 g/kg体重)、中(2 g/kg体重)、高(4 g/kg体重)剂量组,每组10只。模型组和给药组大鼠经滴鼻法给予LPS(3 mg/kg体重)制备大鼠ALI模型,24 h后,给药组分别灌胃相应浓度的穿王消炎粉,模型组和空白组灌胃相同体积的生理盐水。治疗4 d后处死大鼠,分离血清、固定并冻存肺脏组织。测定各组大鼠肺脏组织湿/干重比;HE染色观察大鼠肺脏组织病理学变化;ELISA法检测血清中白细胞介素-1β(IL-1β)、IL-6含量;采用实时荧光定量PCR和Western blotting法检测肺脏组织中IL-1β、IL-6 mRNA表达水平和蛋白表达量。【结果】与空白组相比,LPS模型组大鼠出现肺间质壁增厚,肺泡腔内炎性细胞浸润、出血,肺泡结构破坏等肺损伤症状,肺组织的湿/干重比极显著升高(P<0.01),血清中IL-1β、IL-6含量和肺脏组织中IL-1β、IL-6 mRNA表达水平及蛋白表达量均显著增加(P<0.05)。与LPS模型组相比,各给药组大鼠肺间质增宽现象有所改善,肺组织的湿/干重比、血清和肺脏组织中炎性因子IL-1β、IL-6 mRNA表达水平及蛋白表达量均显著降低(P<0.05)。【结论】穿王消炎粉可有效抑制LPS诱导的急性肺部损伤和炎症程度。

关键词: 穿王消炎粉; LPS; 大鼠; 肺损伤; IL-1β; IL

Abstract: 【Objective】 The experiment was conducted to study the therapeutic effect of Chuanwang Xiaoyan powder on lipopolysaccharide (LPS) induced acute lung injury in rats.【Methods】 60 SD male rats were randomly divided into blank group,levofloxacin-positive drug group,LPS model group,low-dose (1 g/kg BW),medium-dose (2 g/kg BW) and high-dose (4 g/kg BW) Chuanwang Xiaoyan powder groups.Each group contains 10 rats.A rat model of acute lung injury was made with 3 mg/kg LPS in the model group and the administration groups by the nasal drip method for 24 h.After successful modeling,the drug administration groups were given the corresponding doses of drugs by gavage,and the model and blank groups were given the same doses of saline by gavage.After 4 d of treatment,the rats were sacrificed,the serum was separated,and the lung tissues were fixed and cryopreserved.The wet/dry weight ratio of rat lung tissue was detected in each group.HE staining was used to observe the pathological changes of rat lung tissue.ELISA method was used to detect the content of IL-1β and IL-6 in serum,and the mRNA and protein expression levels of IL-1β and IL-6 in the lung tissue were respectively detected by Real-time quantitative PCR and Western blotting.【Results】 Compared with the blank group,rats in the LPS model group had thicker lung interstitial walls,infiltration of inflammatory cells in alveolar cavity,bleeding,and destruction of alveolar structure and other symptoms of pneumonia.The wet/dry weight ratio of lung tissue was increased extremely (P<0.01).The levels of IL-1β and IL-6 in serum,and the mRNA expression levels and protein expression levels of IL-1β and IL-6 in lung tissues were significantly increased (P<0.05).Compared with the LPS model group,the pulmonary interstitial thickening of the rats in the drug treatment groups was alleviated.The wet/dry weight ratio of lung tissue,the expression levels of inflammatory factors IL-1β,IL-6 mRNA and protein expressions in serum and lung tissue were significantly reduced (P<0.05).【Conclusions】 Chuanwang Xiaoyan powder could effectively inhibit LPS induced acute lung injury and inflammation.

Key words: Chuanwang Xiaoyan powder; LPS; rats; lung injury; IL-1β; IL-6

中图分类号:

S853.74

王璐, 胡盼盼, 程佳, 孙盼盼, 孙娜, 范阔海, 尹伟, 李宏全, 孙耀贵. 穿王消炎粉对LPS诱导大鼠急性肺损伤的治疗作用[J]. 中国畜牧兽医, 2022, 49(4): 1568-1576.

WANG Lu, HU Panpan, CHENG Jia, SUN Panpan, SUN Na, FAN Kuohai, YIN Wei, LI Hongquan, SUN Yaogui. Effect of Chuanwang Xiaoyan Powder on LPS Induced Acute Lung Injury in Rats[J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(4): 1568-1576.

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