性成熟前不同日龄伊拉兔睾丸和附睾形态学与组织学特征

doi:10.16431/j.cnki.1671-7236.2022.07.022

摘要/Abstract

摘要： 【目的】探究不同日龄伊拉兔睾丸和附睾组织在性成熟前的变化规律，为其初情期及性成熟的判定提供组织学依据。【方法】将45只伊拉兔随机分为9组，每组5只，从30日龄开始每隔15日采集1组试验兔的睾丸及其附睾，运用形态学与组织解剖学方法对其生长发育规律进行研究分析。【结果】随着日龄的增加，睾丸指数、睾丸重、睾丸长径、睾丸短径及厚径等指标逐步增加，且150日龄时各指标均显著高于其他组（P＜0.05）；30、45、60日龄睾丸内生精小管排列稀疏，75、90、105日龄时睾丸间质成分逐渐增多，管腔逐步形成，120、135、150日龄时间质细胞进一步增生并成群分布，90日龄起出现圆形和长形未成型精子，120日龄的睾丸生精小管、附睾管腔中出现少量成型精子，150日龄时有大量精子密集分布于睾丸管腔内，且在120、135、150日龄时生精小管面积、上皮细胞厚度、支持细胞数均显著大于其他组（P＜0.05）；30日龄以上各组间质细胞个数在各组间差异不显著（P＞0.05），但均显著高于30日龄；120、135、150日龄时附睾头、体、尾的直径均极显著高于其他组（P＜0.05），而附睾头的柱状上皮厚度和纤毛长度则从105日龄起就显著高于30、45、60、75和90日龄组（P＜0.05）。【结论】伊拉兔睾丸和附睾随日龄增加同步发育，在120日龄时达到初情期，在150日龄时睾丸和附睾已发育成熟，基本达到性成熟。

关键词: 伊拉兔; 睾丸; 附睾; 性成熟

Abstract: 【Objective】 This study was aimed to explore the changes of testis and epididymal tissues in different days old Hyla rabbits before sexual maturity to provide histological basis for the determination of their puberty and sexual maturity.【Method】 45 male Hyla rabbits were randomly divided into 9 groups,with 5 in each group,the testis and epididymis of one group of rabbits were collected every 15 days from 30 days of age,and their growth and development rules were examined and analyzed using gross morphology and histoanatomy.【Result】 With the increase of daily age,testicular index,testicular weight,testicular long diameter,testicular short diameter,and testicular thickness were gradually increased,and all the indexes of each group at 150 days of age were significantly higher than those in other groups (P＜0.05).30,45 and 60 days old testis with endophyte sperm tubules were sparsely arranged.The stromal component of the testis of 75,90 and 105 days old were gradually increased and the lumen was gradually formed,the plasmic cells of 120,135 and 150 days old were further proliferated and distributed in groups,round and long-shaped unformed sperm appeared from 90 days old,a small amount of formed sperm appeared in the lumen of the epididymis at the 120 days old testis,and a large number of sperm was densely distributed in the testicular lumen at 150 days old.Moreover,the rabbit sperm tubule area,epithelial cell thickness and number of supporting cells were significantly greater at 120,135 and 150 days old than those in the other groups (P＜0.05).The number of interstitial cells did not differ significantly between the groups above 30 days old (P＞0.05),but all were significantly greater than 30 days old.The diameter of rabbit epididymal head,body and tail at 120,135 and 150 days old were significantly higher than those in the other groups (P＜0.05),the columnar epithelial thickness and cilia length of the epididymal head were significantly higher from 105 days old than in the 30,40,60,75 and 90 days old groups (P＜0.05).【Conclusion】 The testis and epididymis of Hyla rabbits developed synchronously with the increase of age,reaching the initial estrus at 120 days old,and the testis and epididymis basically reached sexual maturity at 150 days old.

Key words: Hyla rabbits; testis; epididymis; sexual maturity

中图分类号:

S829.1

铁雅楠, 杨喜喜, 王志亮, 王欣荣. 性成熟前不同日龄伊拉兔睾丸和附睾形态学与组织学特征[J]. 中国畜牧兽医, 2022, 49(7): 2645-2653.

TIE Yanan, YANG Xixi, WANG Zhiliang, WANG Xinrong. Morphological and Histological Characteristics of Testis and Epididymis of Hyla Rabbits at Different Ages Before Sexual Maturity[J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(7): 2645-2653.

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