绵羊睾丸差异基因及蛋白质互作网络关系研究

doi:10.16431/j.cnki.1671-7236.2022.01.001

中国畜牧兽医 ›› 2022, Vol. 49 ›› Issue (1): 1-11.doi: 10.16431/j.cnki.1671-7236.2022.01.001

绵羊睾丸差异基因及蛋白质互作网络关系研究

刘在霞^1,2, 段仕^1,4, 孙燕勇^1,2, 吕琦¹, 付绍印³, 何小龙³, 张文广^1,2, 刘永斌³

1. 内蒙古农业大学动物科学学院, 内蒙古自治区动物遗传育种与繁殖重点实验室, 呼和浩特 010018;
2. 内蒙古自治区农业基因组大数据工程研究中心, 呼和浩特 010018;
3. 内蒙古自治区农牧业科学院, 呼和浩特 010031;
4. 和田师范专科学校, 和田 848000

收稿日期:2021-07-23 出版日期:2022-01-05 发布日期:2021-12-29
通讯作者: 张文广, 刘永斌 E-mail:actgnmbi@aliyun.com.cn;ybliu117@126.com
作者简介:刘在霞,E-mail:zaixia0120@163.com。
基金资助:
内蒙古自治区科技重大专项（2020ZD0003）；财政部和农业农村部：国家现代农业产业技术体系（CARS-38）；"科技兴蒙"行动重点专项巴彦淖尔国家农业高新技术产业示范区重点项目

Study on Differential Gene and Protein Interaction Network of Testis in Sheep

LIU Zaixia^1,2, DUAN Shi^1,4, SUN Yanyong^1,2, LYU Qi¹, FU Shaoyin³, HE Xiaolong³, ZHANG Wenguang^1,2, LIU Yongbin³

1. Key Laboratory of Animal Genetics, Breeding and Reproduction of the Inner Mongolia Autonomous Region, College of Animal Science and Technology, Inner Mongolia Agricultural University, Hohhot 010018, China;
2. Inner Mongolia Engineering Research Center of Genomic Big Data for Agriculture, Hohhot 010018, China;
3. Inner Mongolia Academy of Agricultural & Animal Husbandry Sciences, Hohhot 010031, China;
4. Hetian Normal College, Hetian 848000, China

Received:2021-07-23 Online:2022-01-05 Published:2021-12-29

摘要/Abstract

摘要： [目的] 整合分析多个绵羊睾丸转录组数据集，揭示绵羊睾丸差异基因及蛋白质互作网络关系，以期探索影响绵羊精子生成的关键基因，为绵羊的繁殖提供理论参考。[方法] 对74个绵羊睾丸转录组进行生物信息学分析，通过limma软件包进行差异基因分析，使用WGCNA构建加权绵羊睾丸差异基因共表达网络，并通过MCODE计算网络中重要基因；利用Metascape进行功能富集分析，利用Cytoscape插件AutoAnnotate识别基因集簇。[结果] 最终筛选到11 884个基因，构建237 366对蛋白质互作关系；对2 058个差异基因构建蛋白质互作网络，产生46 169对蛋白质互作关系，确定了4个得分最高的基因集合。对2 058个差异基因构建加权共表达网络，共获得7个模块，其中Blue模块内有929个基因，功能富集分析发现显著富集于雄性配子产生、繁殖、精子发生、鞭毛运动和AMPK信号通路等，且富集结果高度连接并聚成一个完整的网络，最终确定了25个参与精子发生和精子细胞发育过程的关键基因。[结论] 本研究揭示了绵羊睾丸表达基因的蛋白质互作网络和多维差异基因的蛋白质互作网络，最终找到与睾丸精子发生相关且有互作关系的25个基因，为绵羊繁殖研究提供理论依据。

关键词: 绵羊; 睾丸差异基因; 蛋白质互作网络; 关键基因集; 精子发生

Abstract: [Objective] The research was aimed to integrate and analyze the multiple transcription data sets of testis in sheep, reveal the differential genes and protein interaction network of testis in sheep, and explore the key genes affecting spermatogenesis in sheep, so as to provide theoretical reference for reproduction in sheep. [Method] 74 testis transcriptome in sheep were bioinformatic analyzed, differential genes were carried out by limma package, WGCNA was used to construct the weighted differential genes co-expression network of testis in sheep and MCODE was used to calculate the important genes in the network. Metascape was used for functional enrichment analysis, and Cytoscape plug-in AutoAnnotate was used to identify gene clusters. [Results] 11 884 genes were screened and 237 366 protein interactions were constructed. Protein interaction network were constructed from 2 058 differential genes, and 46 169 protein interaction relationship were generated. 4 gene sets with the highest scores were identified. At the same time, a weighted co-expression network was constructed for 2 058 differential genes, and a total of 7 modules were obtained, among which 929 genes in the Blue module were found to be significantly enriched in male gamete production, reproduction, spermatogenesis, flagellum motility and AMPK signaling pathway, and the enrichment results were highly connected and aggregated into a complete network. As a result, 25 key genes involved in spermatogenesis and sperm cell development. [Conclusion] This study revealed the protein interaction network of genes expressed in testis of sheep and the protein interaction network of multi-dimensional differential genes, finally 25 genes were associated with testis spermatogenesis and interaction relationship, it provided theoretical basis for breeding in sheep.

Key words: sheep; testis differential gene; protein interaction network; key gene set; spermatogenesis

中图分类号:

S814.8

刘在霞, 段仕, 孙燕勇, 吕琦, 付绍印, 何小龙, 张文广, 刘永斌. 绵羊睾丸差异基因及蛋白质互作网络关系研究[J]. 中国畜牧兽医, 2022, 49(1): 1-11.

LIU Zaixia, DUAN Shi, SUN Yanyong, LYU Qi, FU Shaoyin, HE Xiaolong, ZHANG Wenguang, LIU Yongbin. Study on Differential Gene and Protein Interaction Network of Testis in Sheep[J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(1): 1-11.

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绵羊睾丸差异基因及蛋白质互作网络关系研究

Study on Differential Gene and Protein Interaction Network of Testis in Sheep

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