高脂高果糖饮食对老年小鼠视网膜形态结构的影响

doi:10.16431/j.cnki.1671-7236.2022.07.009

摘要/Abstract

摘要： 【目的】探讨高脂高果糖饮食对老年小鼠视网膜形态结构改变的影响。【方法】将12只4周龄的SPF级雄性C57BL/6J小鼠饲养至12月龄后分成普通饮食组（ND）和高脂高果糖饮食组（HFHFD），每组6只，普通饮食组饲喂普通饲料，高脂高果糖饮食组饲喂高脂高果糖饲料，18月龄取眼球，左眼球用眼球固定液固定，右眼球冻存备用；采用HE染色法观察小鼠视网膜组织结构改变情况，采用免疫组织化学法（IHC）检测小鼠视网膜组织中磷酸化乙酰辅酶A羧化酶（p-ACC）表达，采用免疫荧光（IF）法检测视网膜组织中固醇调节元件结合蛋白-1（SREBP1）的表达情况，采用Western bloting法检测p-ACC、p-AMPK、SREBP1蛋白的表达水平。【结果】HE染色结果显示，与普通饮食组相比，高脂高果糖饮食组视网膜组织出现视杆和视椎层厚度不均匀，局部增生，延伸入外颗粒层，细胞排列疏松等现象；免疫组化结果显示，与普通饮食组相比，高脂高果糖饮食组p-ACC表达水平显著下降（P＜0.05）；免疫荧光结果显示，与普通饮食组相比，高脂高果糖饮食组SREBP1蛋白表达显著增加（P＜0.05）；Western bloting结果显示，与普通饮食组相比，高脂高果糖饮食组p-AMPK和p-ACC蛋白表达水平均显著降低（P＜0.05），SREBP1蛋白表达水平显著升高（P＜0.05）。【结论】高脂高果糖饮食可诱导老年小鼠视网膜病变，抑制AMPK的活化，降低p-ACC的表达，升高SREBP1的表达，结果可为进一步探索高脂高果糖饮食影响视网膜结构改变的机制提供参考。

关键词: 高脂高果糖饮食; 视网膜损伤; 脂质沉积

Abstract: 【Objective】 This study was aimed to investigate the effect of high fat and high fructose diet on the morphological changes of retina in elder mice.【Method】 A total of twelve 4-week-old SPF male C57BL/6J mice were fed to 12 months old and then divided into normal diet group (ND) and high fat and high fructose diet group (HFHFD),with 6 mice in each group.The normal diet group was fed with ordinary diet,and the high fat and high fructose diet group was fed with high fat and high fructose diet.The eyeballs were taken at the age of 18 months,the left eyeballs were fixed with eyeball fixation solution,and the right eyeballs were frozen for standby.The structural changes of mouse retina were observed by HE staining,the expression of phosphorylated acetyl CoA carboxylase(p-ACC) was detected by immunohistochemistry (IHC) method,the expression of sterol regulatory element binding protein-1 (SREBP1) was detected by immunofluorescence (IF) method,and the expression of p-ACC,p-AMPK and SREBP1 protein were detected by Western blotting.【Result】 HE staining results showed that compared with normal diet group,the retinal tissues in the high fat and high fructose diet group showed uneven thickness of rods and vertebral layers,local hyperplasia,extension into the outer granular layer,loose arrangement of cells and so on.Immunohistochemistry results showed that compared with normal diet group,the expression of p-ACC in the high fat and high fructose diet group was significantly decreased (P＜0.05).Immunofluorescence results showed that compared with normal diet group,the expression of SREBP1 protein in the high fat and high fructose diet group was significantly increased (P＜0.05).Western bloting results showed that compared with normal diet group,the expression of p-AMPK and p-ACC protein in the high fat and high fructose diet group were significantly decreased (P＜0.05),and SREBP1 protein was significantly increased (P＜0.05).【Conclusion】 Retinopathy in elder mice could be induced by high-fat and high fructose diet,which could inhibit the activation of AMPK,reduce the expression of p-ACC and increase the expression of SREBP1.The results could provide a reference for further exploring the mechanism of high-fat and high fructose diet affecting retinal structural changes.

Key words: high fat and high fructose diet; retinal injury; lipid deposition

中图分类号:

S853.1

张玉珍, 黄敏仪, 陈可纯, 蔡永铭, 杨祎琦. 高脂高果糖饮食对老年小鼠视网膜形态结构的影响[J]. 中国畜牧兽医, 2022, 49(7): 2515-2522.

ZHANG Yuzhen, HUANG Minyi, CHEN Kechun, CAI Yongming, YANG Yiqi. Effects of High Fat and High Fructose Diet on Retinal Morphology in Aged Mice[J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(7): 2515-2522.

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