不同年龄蒙古马睾丸组织形态及生精上皮细胞变化规律研究

doi:10.16431/j.cnki.1671-7236.2023.09.025

摘要/Abstract

摘要： 【目的】探索蒙古马不同年龄阶段睾丸组织形态及生精上皮细胞数量变化规律,从而为蒙古马精子发生相关研究提供理论参考。【方法】采集1、2、3、4、5和6岁蒙古马睾丸组织,通过HE染色对睾丸组织、曲细精管结构进行形态学观察,并统计曲细精管、生精上皮细胞相关参数,探究其发育规律。【结果】睾丸组织学观察结果显示,1岁蒙古马睾丸组织未见成熟精子,曲细精管管腔为实心结构且管径小。2岁时出现少量成熟精子,曲细精管部分呈现空腔结构,随蒙古马年龄增长成熟精子数量逐渐增多。曲细精管直径、面积及生精细胞、支持细胞数量均随蒙古马年龄增长而增加,6岁时达最高水平,且均极显著高于1岁(P＜0.01)。【结论】蒙古马2岁时进入性成熟阶段,且随着精子发生的不断进行,各类生精上皮细胞开始出现,睾丸曲细精管面积和直径、生精细胞和支持细胞数量随年龄增长而不断增加。研究结果为确定蒙古马育种周期、提高繁殖性能等研究提供理论参考。

关键词: 蒙古马; 睾丸; 曲细精管; 生精上皮细胞

Abstract: 【Objective】 This study was aimed to explore the morphological changes of testis and the number of spermatogenic epithelial cells at different ages of Mongolian horses,so as to provide theoretical reference for the study of spermatogenesis of Mongolian horses.【Method】 Testis tissues of Mongolian horses aged 1,2,3,4,5 and 6 years old were collected,and morphological observation was made on testis tissues and convoluted spermatogenic epithelium structures by HE staining,and relevant parameters of convoluted spermatogenic epithelium cells were collected to explore their development rules.【Result】 Testis histological observation showed that there was no mature sperm in the testis tissue of the 1 year old Mongolian horse,and the lumen of the fine ductus was solid with small diameter.At the age of 2 years old,a small amount of mature sperm appeared,and the curved spermatozoa part showed a cavity structure.The number of mature sperm gradually increased with the age of Mongolian horse.The diameter and area of seminiferous tubule and the number of spermatogenic cells and Sertoli cells increased with age,and reached the highest level at 6 years old,and was extremely significantly higher than that at 1 year old (P<0.01).【Conclusion】 The Mongolian horse reached the stage of sexual maturity at 2 years old,and with the continuous progress of spermatogenesis,various spermatogenic epithelial cells began to appear,the area and diameter of seminiferous tubule of testis,the number of spermatogenic cells and Sertoli cells increased with age.The results provided theoretical reference for determining the breeding cycle and improving the breeding performance of Mongolian horse.

Key words: Mongolian horse; testicles; seminiferous tubules; spermatogenic epithelial cells

中图分类号:

S814.8

刘源壹, 贾紫洁, 李昕俞, 张磊, 格日乐其木格, 芒来, 杜明. 不同年龄蒙古马睾丸组织形态及生精上皮细胞变化规律研究[J]. 中国畜牧兽医, 2023, 50(9): 3688-3694.

LIU Yuanyi, JIA Zijie, LI Xinyu, ZHANG Lei, BOU Gerelchimeg, MANG Lai, DU Ming. Morphological and Spermatogenic Epithelial Cells of Testis of Mongolian Horse at Different Ages[J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(9): 3688-3694.

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