包被叶酸和包被牛磺酸对热应激种公羊精液品质、血清和精浆抗氧化能力及激素水平的影响

doi:10.16431/j.cnki.1671-7236.2023.07.016

摘要/Abstract

摘要： 【目的】探讨包被叶酸（coated folic acid，CFA）和包被牛磺酸（coated taurine，CTau）对热应激种公羊精液品质、血清和精浆抗氧化能力及激素水平的影响，为缓解夏季种公羊的热应激及改善精液品质提供参考依据。【方法】选择体重及年龄相近、体况良好的成年杜泊种公羊20只，随机分为4组：对照组饲喂基础饲粮（0 mg/d CFA和CTau）；CFA组在基础饲粮中添加291.6 mg/d CFA和0 g/d CTau；CTau组在基础饲粮中添加0 mg/d CFA+2.916 g/d CTau；CFA+CTau组在基础饲粮中添加291.6 mg/d CFA+2.916 g/d CTau。试验期共75 d，其中预试期15 d，正试期60 d。在正试期第60天采集种公羊血液和精液，检测精液品质指标（射精量、精子活率、精子密度、快速前向运动精子百分比、精子DNA完整率、质膜完整率和精子畸形率），血清和精浆中总抗氧化能力（T-AOC）、过氧化氢酶（CAT）、超氧化物歧化酶（SOD）活性和丙二醛（MDA）含量，以及促卵泡素（FSH）、促黄体素（LH）、睾酮（T）、皮质醇（COR）、三碘甲状腺原氨酸（T₃）和甲状腺素（T₄）水平。【结果】①饲粮中添加CFA和CTau对精子活率有显著互作效应（P<0.05），对精子密度、精子DNA完整率和质膜完整率有极显著互作效应（P<0.01）。与对照组相比，CFA、CTau和CFA+CTau组精子活率、精子密度、精子DNA完整率和质膜完整率均极显著升高（P<0.01），CTau和CFA+CTau组精子畸形率极显著降低（P<0.01）。②与对照组相比，CFA和CFA+CTau组精浆中MDA含量显著降低（P<0.05），其余指标均无显著差异（P>0.05）。③饲粮中添加CFA和CTau对精浆中COR水平有显著互作效应（P<0.05）。与对照组相比，CFA组血清中COR水平、CFA+CTau组血清中T₄水平均显著升高（P<0.05）；CTau和CFA+CTau组精浆中LH水平显著降低（P<0.05），CFA、CTau和CFA+CTau组精浆中T₃水平显著升高（P<0.05）。【结论】饲粮中添加CFA或CTau可改善热应激种公羊抗氧化能力和激素分泌水平，从而提高精液品质，二者同时添加效果更好。

关键词: 种公羊; 热应激; 包被叶酸; 包被牛磺酸; 精液品质

Abstract: 【Objective】 This study was aimed to investigate the effects of coated folic acid (CFA) and coated taurine (CTau) on semen quality, antioxidant capacity and hormone level in serum and seminal plasma of the heat stressed rams, so as to provide a reference for the relief the heat stress and improvement of semen quality of rams in summer.【Method】 Twenty adult Dorper rams in good body condition with similar body weight and age were selected and randomly divided into 4 groups.The rams in control group were fed with the basal feed(0 mg/d CFA and CTau), the rams in CFA, CTau and CFA+CTau groups were fed the basal feed plus 291.6 mg/d CFA+0 g/d CTau, 0 mg/d CFA+2.916 g/d CTau, and 291.6 mg/d CFA+2.916 g/d CTau, respectively.The trial period was 75 d, with a pretrial period of 15 d and a regular trial period of 60 d.Blood and semen of ram were collected on the 60th day of the trial period, the semen quality indicators (sperm collection volume, sperm viability, sperm concentration, percentage of fast forward progressive motility of sperm, DNA integrity of sperm, plasma membrane integrity and sperm malformation rate), and the total antioxidant capacity (T-AOC), the activity of catalase (CAT) and superoxide dismutase (SOD), the content of malondialdehyde (MDA), and the levels of follicle-stimulating hormone (FSH), luteinizing hormone (LH), testosterone(T), cortisol(COR), triiodothyronine(T₃) and thyroxine(T₄) in serum and seminal plasma were detected.【Result】 ①Dietary CFA and CTau had significant interaction effects on sperm viability (P<0.05), and had extremely significant interaction effects on sperm concentration, DNA integrity of sperm and plasma membrane integrity (P<0.01).Compared with control group, the sperm viability, sperm concentration, DNA integrity of sperm and plasma membrane integrity in CFA, CTau and CFA+CTau groups were extremely significantly increased (P<0.01), the sperm malformation rate in CTau and CFA+CTau groups were extremely significantly decreased (P<0.01).② Compared with control group, the MDA content of seminal plasma in CFA and CFA+CTau groups were significantly decreased (P<0.05), but the other antioxidant indexes had no significant effects (P>0.05).③ Dietary CFA and CTau had a significant interaction effect on COR level in seminal plasma (P<0.05).Compared with control group, the COR level of serum in CFA group and the T₄ level of serum in CFA+CTau group were significantly increased (P<0.05), respectively, the LH level of seminal plasma in CTau and CFA+CTau groups were significantly decreased (P<0.05), the T₃ level of seminal plasma in CFA, CTau and CFA+CTau groups were significantly increased (P<0.05).【Conclusion】 Dietary supplementation of CFA or CTau could improve the antioxidant capacity and hormone secretion levels, and improve the semen quality of heat stressed rams, and adding together were more effective.

Key words: rams; heat stress; coated folic acid (CFA); coated taurine (CTau); semen quality

中图分类号:

S821.3

雷铭凯, 班斌, 殷炜琦, 张俊梅, 石攀峰, 王丽, 周扬, 张春香, 李碧波. 包被叶酸和包被牛磺酸对热应激种公羊精液品质、血清和精浆抗氧化能力及激素水平的影响[J]. 中国畜牧兽医, 2023, 50(7): 2755-2765.

LEI Mingkai, BAN Bin, YIN Weiqi, ZHANG Junmei, SHI Panfeng, WANG Li, ZHOU Yang, ZHANG Chunxiang, LI Bibo. Effects of Coated Folic Acid and Coated Taurine on Semen Quality, Serum and Seminal Plasma Antioxidant Capacity and Hormone Level in Heat Stressed Rams[J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(7): 2755-2765.

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