雷州山羊、努比亚羊及其杂交F1代在热应激环境下生理和血液生化指标的对比研究

doi:10.16431/j.cnki.1671-7236.2021.10.013

中国畜牧兽医 ›› 2021, Vol. 48 ›› Issue (10): 3625-3634.doi: 10.16431/j.cnki.1671-7236.2021.10.013

雷州山羊、努比亚羊及其杂交F1代在热应激环境下生理和血液生化指标的对比研究

童雄¹, 闵力¹, 孙浩¹, 徐斌¹, 丁迪云¹, 李大刚¹, 王刚^1,2

1. 广东省农业科学院动物科学研究所, 畜禽育种国家重点实验室, 广东省畜禽育种与营养研究重点实验室, 广州 510640;
2. 广东省农业科学院动物卫生研究所, 岭南现代农业科学与技术广东省实验室茂名分中心, 广东省畜禽疫病防治研究重点实验室, 广州 510640

收稿日期:2021-04-01 出版日期:2021-10-20 发布日期:2021-09-30
通讯作者: 李大刚, 王刚 E-mail:609048302@qq.com;wgwg2004@aliyun.com
作者简介:童雄(1986-),男,湖北孝感人,博士,助理研究员,研究方向:动物遗传育种,E-mail:tongxiong@gdaas.cn
基金资助:
广东省农业科学院院长基金项目（201809）；广东省自然科学基金项目（2018A030313002）；提升市县农业科技能力促进优势产业发展创新模式研究项目（TS-1-6-13）

Comparative Study on Physiological and Blood Biochemical Indexes of Leizhou, Nubia and Their F1 Crossbred Goats Under Heat Stress

TONG Xiong¹, MIN Li¹, SUN Hao¹, XU Bin¹, DING Diyun¹, LI Dagang¹, WANG Gang^1,2

1. State Key Laboratory of Livestock and Poultry Breeding, Guangdong Key Laboratory of Animal Breeding and Nutrition, Institute of Animal Science, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China;
2. Maoming Sub-center Guangdong Laboratory for Lingnan Modern Agricultural Science and Technology, Key Laboratory of Livestock Disease Prevention of Guangdong Province, Institute of Animal Health, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China

Received:2021-04-01 Online:2021-10-20 Published:2021-09-30

摘要/Abstract

摘要： 本试验旨在研究广东地区夏季热应激对雷州山羊、努比亚羊及其杂交F1代生理和血液生化指标的影响，进而评估3个类群羊之间热适应性的差异。试验以各个类群5月龄的阉羊为研究对象，在广东夏季（2019年7月）全天温湿度指数（THI）均值为84.30的热应激环境下，正试期第1和2天在08：00、14：00、20：00 3个时间点测定羊群的4项生理指标，第3天采集羊群血液进行29项生化指标的测定分析。结果显示，3个类群羊的表皮温度（ST）、直肠温度（RT）、呼吸频率（RR）和心率（HR）随着08：00至14：00 THI的增高而增高，随着14：00至20：00 THI的下降而下降。在08：00和14：00平均THI高于82的热应激条件下，雷州山羊的RT和RR极显著或显著低于努比亚羊（P<0.01；P<0.05）。白细胞分类指标中，雷州山羊的嗜中性粒细胞比例（NEUTr）在3个类群羊中最高，且显著高于努比亚羊（P<0.05）。与此相反，雷州山羊的淋巴细胞比例（LYMPHr）在三者中最低，且显著低于努比亚羊（P<0.05）。血液电解质指标中，雷州山羊的钾离子（K⁺）在3个类群中最高，且极显著高于努比亚羊（P<0.01）。血清酶类活性指标中，努比亚羊的乳酸脱氢酶（LDH）活性在3个类群中最高，且显著或极显著高于其他2个类群羊（P<0.05；P<0.01）。相比雷州山羊和杂交F1代，努比亚羊的丙氨酸氨基转移酶（ALT）活性更高，这与LDH的趋势一致，但其仅显著高于杂交F1代（P<0.05）。对比血清酶类活性指标（ALT和LDH），三碘甲状腺原氨酸（T₃）的激素水平在3个类群羊之间呈现相反的趋势，努比亚羊的T₃最低，且显著低于雷州山羊（P<0.05）。应用3个类群羊组间差异显著性的血液生化指标进行PCA聚类分析时发现，雷州山羊与努比亚羊之间存在明显的群体分离，而杂交F1代介于2个亲本群之间。综合以上结果，试验羊在试验期间全天长时间处于热应激状态；试验羊的生理和血液生化指标受到热应激的影响，且反映出类群之间存在耐热性差异，雷州山羊的耐热性优于努比亚羊，而杂交F1代的耐热性介于2个亲本之间。

关键词: 热应激; 雷州山羊; 努比亚羊; 生理指标; 血液生化指标

Abstract: The purpose of this experiment was to study the effects of heat stress on physiological and blood biochemical indexes of Leizhou, Nubia and their hybrid F1 goats in Guangdong. Differences of heat adaptation performance were evaluated among the three groups. In this experiment, five-month-old wethers of each group were selected as the research objects. In a heat stress environment with the mean daily temperature humidity index (THI) of 84.30 in Guangdong summer (July 2019), four physiological indexes were measured at 08:00, 14:00 and 20:00 on the first two days of the experimental period, and 29 biochemical indexes were detected after blood collection on the third day of the experiment period. The results showed that skin temperature (ST), rectum temperature (RT), respiratory rate (RR) and heart rate (HR) of the three groups increased with the increase of THI from 08:00 to 14:00, and decreased with the decrease of THI from 14:00 to 20:00. When the mean THI was higher than 82 at 08:00 and 14:00, RT and RR of Leizhou goats were extremely significantly or significantly lower than those of Nubia goats (P<0.01;P<0.05). Among leukocyte classification indexes, neutrophil ratio (NEUTr) of Leizhou goats was the highest, and significantly higher than that of Nubia goats (P<0.05). On the contrary, lymphocyte ratio (LYMPHr) of Leizhou goats was the lowest among the three groups, and significantly lower than that of Nubia goats (P<0.05). Among blood electrolyte indexes, potassium ion (K⁺) of Leizhou goats was the highest among the three groups, and was extremely significantly higher than that of Nubia goats (P<0.01). Among serum enzyme indexes, lactate dehydrogenase (LDH) of Nubia goats was the highest among the three groups, and was significantly or extremely significantly higher than that of the other two groups (P<0.05;P<0.01). Alanine aminotransferase (ALT) of Nubia goats was higher than that of Leizhou goats and hybrid F1 goats, which was consistent with the trend of LDH, but it was only significantly higher than that of hybrid F1 goats (P<0.05). By comparing the two serum enzyme content indexes (ALT and LDH), hormone level of triiodothyronine (T₃) showed an opposite trend among the three groups, and the T₃ level of Nubia goats was the lowest, and significantly lower than that of Leizhou goats (P<0.05). The results of PCA cluster analysis showed that there was a significant population separation between Leizhou goats and Nubia goats, and hybrid F1 goats were between the two parent populations. Based on the above results, the experimental goats were in a heat stress state throughout the whole experiment period. Effects of heat stress on the physiological and blood biochemical indexes of the studied goats were identified, and which reflected the difference of heat resistance among groups. The heat resistance of Leizhou goats was better than that of Nubia goats, while the heat resistance of hybrid F1 goats was between the two parent goats.

Key words: heat stress; Leizhou goat; Nubia goat; physiological index; blood biochemical index

中图分类号:

S827

童雄, 闵力, 孙浩, 徐斌, 丁迪云, 李大刚, 王刚. 雷州山羊、努比亚羊及其杂交F1代在热应激环境下生理和血液生化指标的对比研究[J]. 中国畜牧兽医, 2021, 48(10): 3625-3634.

TONG Xiong, MIN Li, SUN Hao, XU Bin, DING Diyun, LI Dagang, WANG Gang. Comparative Study on Physiological and Blood Biochemical Indexes of Leizhou, Nubia and Their F1 Crossbred Goats Under Heat Stress[J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(10): 3625-3634.

参考文献

[1] SEJIAN V, SRIVASTAVA R S.Effects of melatonin on adrenal cortical functions of Indian goats under thermal stress[J]. Veterinary Medicine International, 2010, 2010(1-2):348919.
[2] SALAMA A, CAJA G, HAMZAOUI S, et al.Different levels of response to heat stress in dairy goats[J]. Small Ruminant Research, 2014, 121(1):73-79.
[3] MCMANUS C, PALUDO G R, LOUVANDINI H, et al.Heat tolerance in Brazilian sheep:Physiological and blood parameters[J]. Tropical Animal Health and Production, 2009, 41(1):95-101.
[4] CASTANHEIRA M, PAIVA S R, LOUVANDINI H, et al.Use of heat tolerance traits in discriminating between groups of sheep in Central Brazil[J]. Tropical Animal Health and Production, 2010, 42(8):1821-1828.
[5] 刘艳芬, 刘铀, 林红英, 等.不同品种山羊与雷州山羊杂交一代生长发育研究[J]. 中国草食动物, 2002, 3:20-23. LIU Y F, LIU Y, LIN H Y, et al.Study on the growth and development of hybrid F1 between different breed goats and Leizhou goats[J]. China Herbivore, 2002, 3:20-23.(in Chinese)
[6] 刘艳芬, 谢为天, 林红英, 等.雷州山羊及其杂交后代的红细胞免疫功能研究[J]. 中国兽医科技, 2002, 1:24-25. LIU Y F, XIE W T, LIN H Y, et al.Study on red blood cell immune function of Leizhou goat and its hybrid progeny[J]. Chinese Journal of Veterinary Science and Technology, 2002, 1:24-25.(in Chinese)
[7] AGRICULTURE ENGINEERING TECHNOLOGY GUIDE.Livestock and Poultry Heat Stress Indices[M].USA:Clemson University, 1990.
[8] RIBEIRO M N, RIBEIRO N L, BOZZI R, et al.Physiological and biochemical blood variables of goats subjected to heat stress——A review[J]. Journal of Applied Animal Research, 2018, 46(1):1036-1041.
[9] MARAI I F M, EL-DARAWANY A A, FADIEL A, et al.Physiological traits as affected by heat stress in sheep——A review[J]. Small Ruminant Research, 2007, 71(1-3):1-12.
[10] ADEDEJI T A.Effect of some qualitative traits and non-genetic factors on heat tolerance attributes of extensively reared West African Dwarf (WAD) goats[J]. International Journal of Applied Agriculture & Apiculture Research, 2012, 8(1):68-81.
[11] GUPTA M, KUMAR S, DANGI S S, et al.Physiological, biochemical and molecular responses to thermal stress in goats[J]. International Journal of Livestock Research, 2013, 3(2):27-38.
[12] 黄德均, 向白菊, 高立芳, 等.热应激对红安格斯牛、抗旱王牛及红抗杂交牛生理指标和血液生化指标的影响[J]. 黑龙江畜牧兽医, 2019, 16:35-39. HUANG D J, XIANG B J, GAO L F, et al.The effect of heat stress on physiological and blood biochemical indexes of Red Angus cattle, Droughtmaster cattle and Red Angus×Droughtmaster hybrid cattle[J]. Heilongjiang Animal Science and Veterinary Medicine, 2019, 16:35-39.(in Chinese)
[13] SHILJA S, SEJIAN V, BAGATH M, et al.Adaptive capability as indicated by behavioral and physiological responses, plasma HSP70 level, and PBMC HSP70 mRNA expression in Osmanabadi goats subjected to combined (heat and nutritional) stressors[J]. International Journal of Biometeorology, 2016, 60(9):1311-1323.
[14] LEITE J H G M, FACANHA D A E, COSTA W P, et al.Thermoregulatory responses related to coat traits of Brazilian native ewes:An adaptive approach[J]. Journal of Applied Animal Research, 2018, 46(1):353-359.
[15] SEIXAS L, MELO C B D, TANURE C B, et al.Heat tolerance in Brazilian hair sheep[J]. Asian-Australasian Journal of Animal Sciences, 2017, 30(4):593.
[16] 张沅.家畜育种学[M].北京:中国农业出版社, 2001. ZHANG Y.Livestock Breeding[M].Beijing:China Agricultural Press, 2001.(in Chinese)
[17] INBARAJ S, SEJIAN V, BAGATH M, et al.Impact of heat stress on immune responses of livestock:A review[J]. Pertanika Journal of Tropical Agricultural Science, 2016, 39(4):459-482.
[18] SINGH K M, SINGH S, GANGULY I, et al.Evaluation of Indian sheep breeds of arid zone under heat stress condition[J]. Small Ruminant Research, 2016, 141:113-117.
[19] CARDOSO C C, PERIPOLLI V, AMADOR S A, et al.Physiological and thermographic response to heat stress in Zebu cattle[J]. Livestock Science, 2015, 182:83-92.
[20] 龚会单, 韩波, 邱静芸, 等.不同环境温度对锦江牛体温调节生理反应和白细胞参数及其淋巴细胞周期的影响[J]. 畜牧与兽医, 2020, 52(1):32-37. GONG H C, HAN B, QIU J Y, et al.The effect of ambient temperature on the physiological thermore-gulation, leukocyte parameters and lymphocyte cell cycle of Jinjiang cattle[J]. Animal Husbandry & Veterinary Medicine, 2020, 52(1):32-37.(in Chinese)
[21] 彭孝坤, 胡建宏, 张恩平.热应激对肉牛和肉羊生理生化指标及外周血miRNA表达水平的影响[J]. 家畜生态学报, 2018, 39(3):1-7. PENG X K, HU J H, ZHANG E P.Effects of heat stress on physiological and biochemical indexes and expression levels of miRNA in peripheral blood of beef cattle, sheep and goats[J]. Journal of Domestic Animal Ecology, 2018, 39(3):1-7.(in Chinese)
[22] ABDEL-FATTAH M S.Changes in body fluids and plasma electrolytes (Na⁺and K⁺) concentrations of balady and damascus goats exposed to heat stress in desert of Sinai, Egypt[J]. World Applied Sciences Journal, 2014, 30(5):534-542.
[23] SEJIAN V, BHATTA R, GAUGHAN J B, et al.Review:Adaptation of animals to heat stress[J]. Animal, 2018, 12(s2):s431-s444.
[24] ŽUBČIĆ D.Some biochemical parameters in the blood of grazing German improved fawn goats from Istria, Croatia[J]. Veterinarski Arhiv, 2001, 71(5):237-244.
[25] 杨前平, 索效军, 张年, 等.乌骨山羊与湖北黑头羊血液生化指标比较分析[J]. 黑龙江畜牧兽医, 2018, 14:71-73. YANG Q P, SUO X J, ZHANG N, et al.Comparative analysis of blood biochemical indexes between black bone goats and Hubei black head goats[J]. Heilongjiang Animal Science and Veterinary Medicine, 2018, 14:71-73.(in Chinese)
[26] PRAGNA P, SEJIAN V, SOREN N M, et al.Summer season induced rhythmic alterations in metabolic activities to adapt to heat stress in three indigenous (Osmanabadi, Malabari and Salem Black) goat breeds[J]. Biological Rhythm Research, 2018, 49(4):551-565.
[27] CAI Y, FU W, CAI D, et al.Ancient genomes reveal the evolutionary history and origin of cashmere-producing goats in China[J]. Molecular Biology and Evolution, 2020, 37(7):2099-2109.

雷州山羊、努比亚羊及其杂交F1代在热应激环境下生理和血液生化指标的对比研究

Comparative Study on Physiological and Blood Biochemical Indexes of Leizhou, Nubia and Their F1 Crossbred Goats Under Heat Stress

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

[1]	武亚南, 石玉祥, 张永英, 刘冠慧, 宋伟光, 钟翠红, 王永霞. 活化Nrf2缓解热应激致肉鸡心肌细胞氧化损伤的研究[J]. 中国畜牧兽医, 2022, 49(9): 3343-3352.
[2]	汪曼丽, 谭子涵, 王帅杰, 李倩, 曾一桐, 王蕾, 赵迪, 张倩, 侯永清. 槲皮素对PEDV感染幼龄仔猪的生长性能、血液生化指标和肠道吸收与屏障功能的影响[J]. 中国畜牧兽医, 2022, 49(9): 3411-3418.
[3]	严啊妮, 沈奔, 王洪荣, 冯春燕, 魏宗友. 过瘤胃葡萄糖和缓释尿素对热应激绵羊生产性能及瘤胃发酵功能的影响[J]. 中国畜牧兽医, 2022, 49(6): 2105-2115.
[4]	刘新勇, 谭正准, 李辉, 黄健, 罗华, 曾令湖, 程隽如, 钟华配, 杨春艳, 王晓丽, 覃广胜. 热应激对摩拉和尼里-拉菲水牛血液生化指标的影响[J]. 中国畜牧兽医, 2022, 49(2): 521-528.
[5]	邹晨, 邢秀梅, 房瑞新, 刘欣, 王洪亮. 不同生长阶段吉林梅花鹿血液生理生化指标分析[J]. 中国畜牧兽医, 2021, 48(6): 2033-2044.
[6]	何文娜, 肖婷, 何颖, 周英宁, 蒋冬福, 卢敬专, 陈忠伟, 胡庭俊. 贞苓增免散对罗非鱼的安全性评价[J]. 中国畜牧兽医, 2021, 48(11): 4004-4013.
[7]	曹英, 库尔班·吐拉克, 杨开伦, 贺贺千, 华兴耀, 李璐, 金大智, 杨玮, 李彦林. 精料中维生素A添加水平对生茸期马鹿日粮营养物质消化、血液生化指标及鹿茸产量的影响[J]. 中国畜牧兽医, 2020, 47(8): 2454-2463.
[8]	张芳毓, 赵中利, 陈龙, 刘臣, 李立佳, 张莹, 刘洪亮, 杨华明, 武斌, 郭洋, 闫晓刚. 人参加工副产物对吉林芦花鸡屠宰性能、胴体品质和血液生化指标的影响[J]. 中国畜牧兽医, 2020, 47(2): 442-451.
[9]	赵小伟, 齐云霞, 杨永新, 吴涛, 黄冬维, 丁海生, 赵辉玲, 程广龙. 中草药添加剂对围产期奶牛血液生化指标及生产性能的影响[J]. 中国畜牧兽医, 2020, 47(12): 3926-3932.
[10]	王彩莲, 吴建平, 刘立山, 郎侠. 冷季牧归补饲精料对高海拔地区藏系绵羊血液生化指标的影响[J]. 中国畜牧兽医, 2020, 47(12): 3933-3943.
[11]	陈少侃, 罗汉鹏, 张国兴, 邱文卿, 董祎鑫, 王炎, 黄锡霞, 王雅春. 热应激条件下荷斯坦奶牛后段肠道差异微生物分析[J]. 《中国畜牧兽医》, 2019, 46(8): 2273-2280.
[12]	赵梦迪, 邸凌峰, 唐泽宇, 李成云. 单宁与饲用纤维素酶对湖羊生长性能、血液生化指标、屠宰性能及器官发育的影响[J]. 《中国畜牧兽医》, 2019, 46(6): 1668-1676.
[13]	熊云霞, 王丽, 温晓鹿, 胡友军, 易宏波, 邱月琴, 杨雪芬, 吴绮雯, 蒋宗勇. 日粮中添加藤茶提取物对猪生长性能、血液生化指标、抗氧化活性的影响[J]. 《中国畜牧兽医》, 2019, 46(5): 1330-1339.
[14]	赵二龙, 冯春燕, 王剑飞, 柏峻, 李艳娇, 李美发, 辛均平, 戈钰, 黎力之, 梁欢, 许兰娇, 瞿明仁, 李婷婷. 一种新型缓释尿素替代日粮中部分豆粕对肉牛生长性能、养分消化率及血液生化指标的影响[J]. 《中国畜牧兽医》, 2019, 46(4): 994-1001.
[15]	姚丽丽, 白霖, 谭盈, 孙嘉琪, 石达友, 郭世宁, 刘翠. 紫锥菊多糖与硫酸化紫锥菊多糖颉颃环磷酰胺对雏鸡免疫抑制作用的研究[J]. 中国畜牧兽医, 2019, 46(10): 3084-3094.