贞苓增免散对罗非鱼的安全性评价

doi:10.16431/j.cnki.1671-7236.2021.11.010

摘要/Abstract

摘要： 试验旨在确定贞苓增免散在临床推荐剂量下对罗非鱼的安全性，研究罗非鱼使用剂量安全范围并综合评价药物的疗效，为临床合理用药提供重要依据与指导。试验分别设置空白组（基础饲粮）、1倍剂量组（含贞苓增免散0.4%）、3倍剂量组（含贞苓增免散1.2%）、5倍剂量组（含贞苓增免散2.0%）和10倍剂量组（含贞苓增免散4.0%）共5个组。连续饲喂30 d，分别在第15和30天检测罗非鱼的临床体征、血液生理生化指标、器官指数，并进行组织病理学观察。结果表明，各剂量组罗非鱼活动、摄食、体色、排泄等均良好，与空白组罗非鱼没有明显区别。0~15 d，增重率和特定生长率在1倍剂量组均最高，且均显著或极显著高于空白组（P<0.05；P<0.01），但其随着贞苓增免散剂量增加而减少；15~30 d贞苓增免散各剂量组增重率均极显著高于空白组（P<0.01）。各剂量组罗非鱼的血液生理生化指标中部分指标与空白组相比有显著差异，但均在正常范围内。0~15 d时各剂量组和15~30 d时1倍剂量组的罗非鱼脾脏指数均显著高于空白组（P<0.05）。显微镜下观察各组罗非鱼肝脏和脾脏组织切片，均未发现药物引起的细胞毒性变化。以上结果说明贞苓增免散在10倍推荐剂量及以下连续饲喂罗非鱼30 d具有较好的安全性，将其作为饲料添加剂能提高罗非鱼生长性能、增强免疫力，对推动罗非鱼养殖快速高效发展具有重要意义。

关键词: 贞苓增免散; 罗非鱼; 生理指标; 生化指标

Abstract: The purpose of the trial was to determine the safety of Zhenling Zengmian powder on tilapia at the recommended clinical dose, study the safe range of tilapia dose and comprehensively evaluate the efficacy of the drug, so as to provide important basis and guidance for clinical rational drug use. Five groups were set up in this experiment, including blank group (basic feed), 1 time dose group (including 0.4% of Zhenling Zengmian powder), 3 times dose group (including 1.2% of Zhenling Zengmian powder), 5 times dose group (including 2.0% of Zhenling Zengmian powder), and 10 times dose group (including 4.0% of Zhenling Zengmian powder). The tilapia were fed continuously for 30 days, and the clinical signs, blood physiological and biochemical indexes, organ index and histopathology of tilapia were detected on the 15th and 30th day, respectively. The results showed that the activity, feeding, body color and excretion of tilapia in each dose group were good, which was not significantly different from that in the blank group. The weight gain rate and specific growth rate were the highest in the 1 time dose group, which were significantly or extremely significantly higher than those in the blank group (P<0.05;P<0.01), but decreased with the increase of the amount of Zhenling Zengmian powder from 0 to 15 days. From 15 to 30 days, the weight gain rate of each dose group of Zhenling Zengmian powder was significantly higher than that of the blank group (P<0.01). Some of the blood physiological and biochemical indexes of tilapia in each dose group were significantly different from those in the blank group, but they were all within the normal range. The tilapia spleen index of each dose group at 0-15 days and the 1 time dose group at 15-30 days were significantly higher than that of the blank group (P<0.05). The liver and spleen sections of tilapia in each group were observed under microscope, and no cytotoxic changes caused by drugs were found. The above results showed that Zhenling Zengmian powder was safe to feed tilapia for continuously 30 days at or below the 10 times of recommended dose. As a feed additive, it could improve the growth performance and enhance immunity of tilapia, which was of great significance to promote the rapid and efficient development of tilapia culture.

Key words: Zhenling Zengmian powder; tilapia; physiological index; biochemical index

中图分类号:

S948

何文娜, 肖婷, 何颖, 周英宁, 蒋冬福, 卢敬专, 陈忠伟, 胡庭俊. 贞苓增免散对罗非鱼的安全性评价[J]. 中国畜牧兽医, 2021, 48(11): 4004-4013.

HE Wenna, XIAO Ting, HE Ying, ZHOU Yingning, JIANG Dongfu, LU Jingzhuan, CHEN Zhongwei, HU Tingjun. Safety Evaluation of Zhenling Zengmian Powder on Tilapia[J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(11): 4004-4013.

参考文献

[1] 罗敏, 顾雯, 何婷, 等.超高效液相色谱-电化学检测法测定女贞子中的3种成分[J]. 中国药学杂志, 2020, 55(24):2006-2011. LUO M, GU W, HE T, et al. Determination of three constituent in Ligustri lucidi Fructus by ultra-high performance liquid chromatography-electrochemical detection[J]. Chinese Pharmaceutical Journal, 2020, 55(24):2006-2011.(in Chinese)
[2] 高赛, 周欣, 陈华国.女贞子化学成分及质量控制研究进展[J]. 中国中医药信息杂志, 2018, 25(12):133-136. GAO S, ZHOU X, CHEN H G.Research progress in chemical components and quality control of Ligustri lucidi Fructus[J] .Chinese Journal of Information on TCM, 2018, 25(12):133-136.(in Chinese)
[3] 战美, 邓雪, 朱迪, 等.女贞子提取物及其成分促骨髓间充质干细胞成骨分化的作用[J]. 中华中医药杂志, 2018, 15(7):36-37. ZHAN M, DENG X, ZHU D, et al. Effects of extracts of Fructus ligustri Lucidi and its components on promoting osteoblast differentiation of bone marrow mesenchymal stem cells[J]. China Journal of Traditional Chinese Medicine and Pharmacy, 2018, 15(7):36-37.(in Chinese)
[4] WANG L L, MA R F, GUO Y B, et al. Antioxidant effect of Fructus ligustri Lucidi aqueous extract in ovariectomized rats is mediated through Nox4-ROS-NF-κB pathway[J]. Front Pharmacol, 2017, 8:266.
[5] 盛东峰, 纪秀娥, 武安泉.日粮中添加女贞子粉对0~4周皖西白鹅生产性能和肠道发育的影响[J]. 饲料工业, 2016, 37(4):41-43. SHENG D F, JI X E, WU A Q.Effects of Ligustrum lucidum in diet on growth performance and intestinal development of Wanxi goose[J]. Feed Industry, 2016, 37(4):41-43.(in Chinese)
[6] 王德强, 佟延南, 李芳远, 等.中草药联用对罗非鱼无乳链球菌作用的研究[J]. 水产养殖, 2017, 38(1):47-51. WANG D Q, TONG Y N, LI F Y, et al. Study on the effect of combined use of Chinese herbal medicine on Streptococcus agalactis in tilapia[J]. Journal of Aquaculture, 2017, 38(1):47-51.(in Chinese)
[7] 刘坤.茯苓多糖PCP-I对炭疽保护性抗原的佐剂效应研究[D].北京:中国人民解放军军事医学科学院, 2017. LIU K.Adjuvant effect of a polysaccharide PCP-I from Poria cocos on anthrax protective antigen[D].Beijing:Academy of Military Medical Sciences, 2017.(in Chinese)
[8] SUN S S, WANG K, MA K, et al. An insoluble polysaccharide from the sclerotium of Poria cocos improves hyperglycemia, hyperlipidemia and hepatic steatosis in ob/ob mice via modulation of gut microbiota[J]. Chinese Journal of Natural Medicines, 2019, 17(1):3-14.
[9] WU K, FAN J, HUANG X, et al. Hepatoprotective effects exerted by Poria cocos polysaccharides against acetaminophen-induced liver injury in mice[J]. International Journal of Biological Macromolecules, 2018, 114:137-142.
[10] 黄武, 徐金龙, 刘建芳, 等.高效液相色谱法检测罗非鱼中丁香酚残留量[J]. 食品安全质量检测学报, 2018, 9(1):103-106. HUANG W, XU J L, LIU J F, et al. Determination of eugenol residues in tilapia products by high performance liquid chromatography[J]. Journal of Food Safety and Quality, 2018, 9(1):103-106.(in Chinese)
[11] AHMED N F N, ESSAM A M, YASSER A E H.Efficacy of dietary Nano-selenium on growth, immune response, antioxidant, transcriptomic profile and resistance of Nile tilapia, Oreochromis niloticus against Streptococcus iniae infection[J]. Fish & Shellfish Immunology, 2019, 94:280-287.
[12] 王洪彬, 朱利霞, 杨楠, 等.常用中草药对水产动物致病菌体外抑菌效果研究[J]. 中国畜牧兽医, 2018, 45(5):1357-1366. WANG H B, ZHU L X, YANG N, et al. Study on antibacterial effects of common Chinese herbs against aquatic animal pathogens in vitro[J]. China Animal Husbandry & Veterinary Medicine, 2018, 45(5):1357-1366.(in Chinese)
[13] GAO L L, LI C, WANG Z M, et al. Ligustri lucidi fructus as a traditional Chinese medicine:A review of its phytochemistry and pharmacology[J]. Natural Product Research, 2015, 29(6):493-510.
[14] 唐荣伟, 李德科, 唐玲, 等.厚朴温中汤加味配伍吗丁啉对功能性消化不良血浆胃肠激素的影响[J]. 中国实验方剂学杂志, 2015, 21(13):174-177. TANG R W, LI D K, TANG L, et al. Impact of Houpu Wenzhong decoction combined with domperidone on plasma gastrointestinal hormone and treatment effect for functional dyspepsia[J] .Chinese Journal of Experimental Traditional Medical Formulae, 2015, 21(13):174-177.(in Chinese)
[15] 李建平, 单安山, 陈志辉, 等.女贞子对断奶仔猪生长性能和血液生化指标的影响[J]. 东北农业大学学报, 2011, 42(9):26-30. LI J P, SHAN A S, CHEN Z H, et al. Effects of Ligustrum lucidum on growth performance and blood biochemical indices of weaned piglets[J]. Journal of Northeast Agricultural University, 2011, 42(9):26-30.(in Chinese)
[16] 张迪.女贞子对断奶仔猪小肠黏膜形态及免疫功能的影响[D].保定:河北农业大学, 2019. ZHANG D.Effect of Ligustrum lucidum on intestinal mucosal structure and immune function in weaned piglets[D].Baoding:Hebei Agricultural University, 2019.(in Chinese)
[17] 龚泽修, 张星, 谢凯, 等.茯苓多糖对母猪繁殖性能、仔猪生长性能及血清免疫指标、抗氧化指标的影响[J]. 饲料研究, 2020, 43(6):27-30. GONG Z X, ZHANG X, XIE K, et al. Effect of pachymaran on sow reproductive performance, piglet growth performance, serum immune indexes and antioxidant indexes[J]. Feed Research, 2020, 43(6):27-30.(in Chinese)
[18] 高鹏辉, 夏九龙, 王志龙, 等.发酵茯苓对断奶仔猪免疫功能的影响[J]. 饲料工业, 2015, 36(21):40-44. GAO P H, XIA J L, WANG Z L, et al. Effects of fermented poria cocos on immune function of weaned piglets[J]. Feed Industry, 2015, 36(21):40-44.(in Chinese)
[19] YU W, WEN G, LIN H, et al. Effects of dietary spirulina platensis on growth performance, hematological and serum biochemical parameters, hepatic antioxidant status, immune responses and disease resistance of coral trout Plectropomus leopardus(Lacepede, 1802)[J]. Fish & Shellfish Immunology, 2018, 74:649-655.
[20] HOLY K Z, SARBAST K K, NATALIE K M, et al. Over-processed meat and bone meal and phytase effects on broilers challenged with subclinical necrotic enteritis:Part 2.Inositol phosphate esters hydrolysis, intestinal permeability, hematology, jejunal gene expression and intestinal morphology[J]. Animal Nutrition, 2020, 6(4):488-498.
[21] LONGVILLE S, STINGACIU L R.Hemoglobin diffusion and the dynamics of oxygen capture by red blood cells[J]. Scientific Reports, 2017, 7(1):1-10.
[22] 李鸣霄, 李红霞, 强俊, 等.饲料中添加刺五加超微粉对吉富罗非鱼生长、脂肪沉积以及非特异性免疫能力的影响[J]. 动物营养学报, 2019, 31(12):5801-5812. LI M X, LI H X, QIANG J, et al. Effects of Acanthopanax senticosus ultrafine powder on growth, fat deposition and non-specific immunity of genetically improved farmed tilapia(Oreochromis niloticus)[J]. Chinese Journal of Animal Nutrition, 2019, 31(12):5801-5812.(in Chinese)
[23] 郭震, 陶永刚, 陈棉彪, 等.新型溴代阻燃剂TBB和TBPH混合物对罗非鱼甲状腺激素和生理生化指标的影响[J]. 生态毒理学报, 2019, 14(5):219-226. GUO Z, TAO Y G, CHEN M B, et al. Effects of brominated flame retardants TBB and TBPH mixture on thyroid hormones and physiological-biochemical indexes in tilapia serum[J]. Asian Journal of Ecotoxicology, 2019, 14(5):219-226.(in Chinese)
[24] 徐莹.女贞子降血糖、降血脂活性及齐墩果酸衍生化研究[D].长春:吉林农业大学, 2016. XU Y.Study on the hypoglycemic and hypolipidemic of Ligustri lucid Ait and derivatization of oleanolic acid[D].Changchun:Jilin Agricultural University, 2016.(in Chinese)
[25] 王秋梅, 李英, 张新党, 等.饲料可消化蛋白和脂肪水平对吉富罗非鱼鱼种生长、物质代谢和抗氧化功能的影响[J]. 云南农业大学学报(自然科学), 2021, 36(2):268-282. WANG Q M, LI Y, ZHANG X D, et al. Effects of digestible protein and lipid levels on the growth performance, substance metabolism and antioxidant capacity of GIFT fingerling(Oreochromis niloticus)[J]. Journal of Yunnan Agricultural University(Natural Science), 2021, 36(2):268-282.(in Chinese)
[26] 王志敬, 刘明珠, 于辉, 等.玉屏风多糖对草鱼生长性能、免疫器官指数和肝胰脏非特异性免疫指标的影响[J]. 动物营养学报, 2016, 28(11):3679-3686. WANG Z J, LIU M Z, YU H, et al. Effects of Yupingfeng polysaccharide on growth performance, immune organ index and non-specific immune indexes of hepatopancreas in grass carp[J]. Chinese Journal of Animal Nutrition, 2016, 28(11):3679-3686.(in Chinese)
[27] 张年, 李兆星, 李娟, 等.茯苓的化学成分与生物活性研究进展[J]. 世界科学技术-中医药现代化, 2019, 21(2):220-233. ZHANG N, LI Z X, LI J, et al. Advances in the research of constituents and pharmacological effects of Poria cocos (Schw.) Wolf[J]. World Science and Technology/Modernization of Traditional Chinese Medicine and Materia Medica, 2019, 21(2):220-233.(in Chinese)
[28] 马钊, 张艺杰, 赵硕, 等.贞苓增免散对罗非鱼生长性能及免疫功能的影响[J]. 饲料研究, 2020, 43(12):46-50. MA Z, ZHANG Y J, ZHAO S, et al. Effect of Zhenlingzengmian powder on growth performance and immune function of tilapia[J]. Feed Research, 2020, 43(12):46-50.(in Chinese)