槲皮素对PEDV感染幼龄仔猪的生长性能、血液生化指标和肠道吸收与屏障功能的影响

doi:10.16431/j.cnki.1671-7236.2022.09.015

摘要/Abstract

摘要： 【目的】试验旨在探讨槲皮素在防治仔猪感染猪流行性腹泻病毒（PEDV）中的作用。【方法】选取18头体重相近、健康的7日龄仔猪（杜×长×大），随机分为3组（对照组、PEDV组和PEDV+槲皮素组），每组6个重复，每个重复1头猪，试验期共11 d。经过3 d适应期后，于试验第4~10天给PEDV+槲皮素组仔猪口腔灌服10 mg/kg BW的槲皮素，其他组仔猪口腔灌服等体积的人工乳，于试验第8天给PEDV组与PEDV+槲皮素组仔猪口腔灌服1×10^4.5TCID₅₀的PEDV，对照组灌服等体积的PBS溶液。试验第11天早上空腹称重，全部仔猪灌服D-木糖（0.1 g/kg BW），1 h后经前腔静脉采血，检测血液生化指标、血浆二胺氧化酶（DAO）活性和D-木糖含量；将所有仔猪屠宰取空肠黏膜，检测肠道屏障相关基因，包括肠绒毛蛋白（villin），紧密连接蛋白-1（claudin-1），闭合蛋白（occludin）和肠型脂肪酸结合蛋白（iFABP）的相对表达量。【结果】与对照组相比，PEDV组仔猪平均日增重显著下降（P<0.05），粪便评分显著升高（P<0.05）；血液中高密度脂蛋白含量显著降低（P<0.05），肌酐和尿素氮的含量显著提高（P<0.05）；血浆中D-木糖含量显著降低（P<0.05）；空肠claudin-1、iFABP基因的相对表达量显著下调（P<0.05）。与PEDV组相比，PEDV+槲皮素组仔猪平均日增重和血浆D-木糖含量显著升高（P<0.05）；血液中肌酐和尿素氮的含量显著降低（P<0.05）；空肠claudin-1、villin和iFABP基因的相对表达量显著上调（P<0.05）。【结论】 10 mg/kg BW槲皮素可在一定程度上缓解PEDV感染导致的仔猪生长抑制和肾功能损伤，增强肠道吸收与屏障功能。

关键词: 槲皮素; 猪流行性腹泻病毒(PEDV); 仔猪; 生长性能; 血液生化指标; 肠道吸收; 肠道屏障

Abstract: 【Objective】 The study was to investigate the role of quercetin in the prevention and treatment of Porcine epidemic diarrhea virus (PEDV) infection in piglets.【Method】 Eighteen healthy weaned piglets (Ducoc×Landrace×Yorkshire, 7-day-old neonatal) with similar body weight were selected and randomly divided into 3 groups (control, PEDV and PEDV+ quercetin groups), with 6 replicates in each group and one piglet per replicate, the trial lasted for 11 d.After 3 d acclimation period, piglets in the PEDV+ quercetin group were orally administered with 10 mg/kg BW of quercetin during the days 4-10 of the experiment, the other groups of piglets were given the same volume of artificial milk.On the day 8 of the experiment, the piglets in PEDV and PEDV+ quercetin groups were orally administered with 1×10^4.5 TCID₅₀ PEDV, and the control group was given with the same volume of PBS.On the day 11 of the experiment, all piglets were weighed and orally administered with D-xylose (0.1 g/kg BW) on an empty stomach.One hour later, jugular vein blood samples were harvested to detect blood biochemical indexes, plasma diamine oxidase (DAO) activity and D-xylose content, and then all piglets were slaughtered and jejunal mucosa was taken to detect the relative expression of intestinal barrier function related genes, including villin, claudin-1, occludin and intestinal fatty acid binding protein (iFABP).【Result】 Compared with the control group, in PEDV group the average daily gain of piglets was significantly decreased (P<0.05), the fecal score was significantly increased (P<0.05);The content of HDL in blood was decreased significantly (P<0.05), and the contents of creatinine and urea nitrogen were increased significantly (P<0.05);The content of D-xylose in plasma was decreased significantly (P<0.05);The relative expressions of claudin-1 and iFABP genes in jejunum were significantly down-regulated(P<0.05).Compared with PEDV group, in PEDV +quercetin group the average daily gain of piglets and the content of D-xylose in plasma were significantly increased (P<0.05);The contents of creatinine and urea nitrogen in blood were significantly decreased (P<0.05);The relative expressions of claudin-1, villin and iFABP genes in jejunum were significantly up-regulated (P<0.05).【Conclusion】 10 mg/kg BW quercetin could alleviate the growth inhibition and renal function injury of piglets caused by PEDV infection to a certain extent, and improve the intestinal absorption and barrier function.

Key words: quercetin; Porcine epidemic diarrhea virus(PEDV); piglets; growth performance; blood biochemical indexes; intestinal absorption; intestinal barrier

中图分类号:

S816.7

汪曼丽, 谭子涵, 王帅杰, 李倩, 曾一桐, 王蕾, 赵迪, 张倩, 侯永清. 槲皮素对PEDV感染幼龄仔猪的生长性能、血液生化指标和肠道吸收与屏障功能的影响[J]. 中国畜牧兽医, 2022, 49(9): 3411-3418.

WANG Manli, TAN Zihan, WANG Shuaijie, LI Qian, ZENG Yitong, WANG Lei, ZHAO Di, ZHANG Qian, HOU Yongqing. Effects of Quercetin on Growth Performance, Blood Biochemical Indexes and Intestinal Absorption and Barrier Function in Piglets Infected with PEDV[J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(9): 3411-3418.

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