苜蓿草粉对育肥猪生长性能、血清抗氧化及免疫性能相关指标的影响

doi:10.16431/j.cnki.1671-7236.2022.03.017

摘要/Abstract

摘要： 【目的】该试验通过研究添加不同比例苜蓿草粉对育肥猪生长性能、血清生化指标、抗氧化性能以及免疫指标的影响，以探明育肥猪饲粮中苜蓿草粉的较优添加比例。【方法】选取健康、平均体重（75.5±1.03） kg的杜×长×大三元杂交猪120头，随机均分为4组，每组6个重复，1个重复1栏。分别在基础饲粮中添加0（对照组）、5%、10%、15%的苜蓿草粉进行饲喂试验，预试期7 d，正式期45 d。测定每头猪初始体重、终末体重及每栏日采食量；试验结束后，每栏随机抽取1头猪，通过前腔静脉采血10 mL，测定血清生化指标、抗氧化指标及免疫球蛋白含量。【结果】与对照组相比，各试验组平均日增重均显著降低（P<0.05），5%和10%苜蓿组料重比（F/G）无显著差异（P>0.05），而15%苜蓿组料重比显著提高（P<0.05）；各试验组抗氧化物歧化酶（SOD）活性均显著提高（P<0.05）；5%苜蓿组甘油三酯（TG）含量显著提高（P<0.05），丙二醛（MDA）含量显著降低（P<0.05）；10%苜蓿组总抗氧化能力（T-AOC）和过氧化氢酶（CAT）活性均显著提高（P<0.05），MDA含量显著降低（P<0.05）。【结论】添加15%苜蓿草粉会显著提高育肥猪料重比，添加5%、10%苜蓿草粉能够提高抗氧化能力。推荐在育肥猪饲粮中苜蓿草粉添加量以5%~10%为宜。

关键词: 苜蓿草粉; 育肥猪; 生长性能; 血清生化指标; 免疫性能; 抗氧化指标

Abstract: 【Objective】 The effects of adding different proportions of alfalfa meal on the growth performance, serum biochemical indicators, antioxidant properties and immune indicators of fattening pigs were studied in this experiment, in order to find out the optimal addition ratio of alfalfa meal in the diets of fattening pigs.【Method】 120 Du×Long×Large pigs with an average weight of (75.5±1.03) kg were selected and randomly divided into 4 groups, with 6 replicates in each group, and 1 pen per replicate.0 (control group), 5%, 10%, and 15% alfalfa meal were added to the basal diets for feeding experiments.The pre-test period was 7 days and the official period was 45 days.The initial weight, final weight of each pig and daily feed intake per pen were determined.After the test, 1 pig was randomly selected from each pen, 10 mL of blood was collected from anterior vena cava to determine the serum biochemical indexes, antioxidant indexes and immunoglobulin content.【Results】 Compared with control group, the average daily gain of each experimental group was significantly reduced (P<0.05).The feed-to-gain ratio (F/G)of 5% and 10% alfalfa groups had no significant difference (P>0.05), while it was significantly increased in 15% alfalfa group (P<0.05).The activities of antioxidant dismutase (SOD) were significantly increased in 3 experimental groups (P<0.05).The triglyceride (TG) content was significantly increased (P<0.05), and the content of malondialdehyde (MDA) was significantly reduced (P<0.05) in 5% alfalfa group.The total antioxidant capacity (T-AOC) and catalase (CAT) activity were significantly increased (P<0.05), while the content of MDA was significantly reduced (P<0.05) in 10% alfalfa group.【Conclusion】 Adding 15% alfalfa meal would significantly increase F/G of fattening pigs, and the addition of 5% and 10% alfalfa could increase the antioxidant capacity.It was recommended that 5%-10% alfalfa meal should be added to the diet of finishing pigs.

Key words: alfalfa meal; finishing pigs; growth performance; serum biochemical index; immune performance; antioxidant index

中图分类号:

S816.4

蒋恒, 王怀树, 卓娜, 王建国, 任佳, 高如军, 武玉平, 高凤芹. 苜蓿草粉对育肥猪生长性能、血清抗氧化及免疫性能相关指标的影响[J]. 中国畜牧兽医, 2022, 49(3): 953-961.

JIANG Heng, WANG Huaishu, ZHUO Na, WANG Jianguo, REN Jia, GAO Rujun, WU Yuping, GAO Fengqin. Effects of Alfalfa Meal on Growth Performance, Serum Related Indexes of Serum Antioxidant and Immune Performance in Finishing Pigs[J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(3): 953-961.

参考文献

[1] 宋增廷, 王华朗, 王立志, 等.我国家禽非常规饲料资源的开发与利用[J].动物营养学报, 2015, 27(1):1-7. SONG Z T, WANG H L, WANG L Z, et al.Development and utilization of unconventional feed resources for poultry in China[J].Chinese Journal of Animal Nutrition, 2015, 27(1):1-7.(in Chinese)
[2] 黄勇, 马娇丽, 王启贵, 等.青绿甜高粱秸秆替代部分全价饲粮对鹅生长性能、屠宰性能及肉品质的影响[J].畜牧兽医学报, 2017, 48(3):483-491. HUANG Y, MA J L, WANG Q G, et al.A Substitution effect of green sweet sorghum stalks for diet on the growth performance, carcass yields and meat quality in geese[J].Chinese Journal of Animal and Veterinary Sciences, 2017, 48(3):483-491.(in Chinese)
[3] 吕先召, 王成章, 邱晓东, 等.苜蓿草粉对育肥猪生长性能、血清生化指标、胴体品质及经济效益的影响[J].动物营养学报, 2018, 30(5):1693-1702. LYU X Z, WANG C Z, QIU X D, et al.Effects of alfalfa meal on growth performance, serum biochemical indexes, carcass quality and economic benefit of finishing pigs[J].Chinese Journal of Animal Nutrition, 2018, 30(5):1693-1702.(in Chinese)
[4] 李红, 杨伟光.苜蓿草对黑龙江省发展节粮型畜牧业的战略意义[A].中国畜牧业协会、中国草学会第三届中国苜蓿发展大会论文集[C].2010. LI H, YANG W G.The Strategic significance of alfalfa to the development of food-saving animal husbandry in Heilongjiang province[A].Proceedings of the Third China Alfalfa Development Conference, China Animal Agriculture Association, Chinese Grassland Society[C].2010.(in Chinese)
[5] 王明进, 熊先勤, 何虎, 等.紫花苜蓿不同生长期收割产量及营养分析[J].黑龙江畜牧兽医, 2015, 21:143-146. WANG M J, XIONG X Q, HE H, et al.Analysis on the yield and nutrition of alfalfa(Medicigo sativa L.) harvested in different growing periods[J].Heilongjiang Animal Science and Veterinary Medicine, 2015, 21:143-146.(in Chinese)
[6] 孔凡勇, 许娅虹, 周仕钰, 等.紫花苜蓿对DLY生长育肥猪生长性能及胴体品质影响试验[A].第六届云南省科协学术年会暨红河流域发展论坛论文集——专题一:红河流域特色产业转型升级[C].2016. KONG F Y, XU Y H, ZHOU S Y, et al.Effect of alfalfa on the growth performance and carcass quality of DLY growing-finishing pigs[A].Proceedings of the Sixth Yunnan Association for Science and Technology Annual Conference and Red River Basin Development Forum——Special Topic 1:Transformation and Upgrading of the Characteristic Industries of the Red River Basin[C].2016.(in Chinese)
[7] 顾丽群, 杨正德, 王嘉福, 等.饲粮中添加紫花苜蓿和苦荞对猪生产性能的影响[J].贵州农业科学, 2011, 39(11):158-161. GU L Q, YANG Z D, WANG J F, et al.Effect of daily ration containing alfalfa and buckwheat on productive performance of emasculated boars[J].Guizhou Agricultural Sciences, 2011, 39(11):158-161.(in Chinese)
[8] 惠铄智, 杨海天, 孔祥杰, 等.苜蓿草粉对松辽黑猪生长性能、屠宰性能及血清生化指标的影响[J].黑龙江畜牧兽医, 2018, 4:159-162. HUI S Z, YANG H T, KONG X J, et al.Effect of alfalfa meal on growth performance, slaughter performance and serum biochemical indexes of Songliao Black pig[J].Heilongjiang Animal Science and Veterinary Medicine, 2018, 4:159-162.(in Chinese)
[9] 王成章, 徐向阳, 杨雨鑫, 等.苜蓿草粉对肥育猪胴体品质及血清指标的影响[J].中国农业科学, 2008, 5:1554-1559. WANG C Z, XU X Y, YANG Y X, et al.Study on effects of alfalfa meal on carcass quality and serum items of fattening pigs[J].Scientia Agricultura Sinica, 2008, 5:1554-1559.(in Chinese)
[10] WVSTHOLZ J, CARRASCO S, BERGER U, et al.Fattening and slaughtering performance of growing pigs consuming high levels of alfalfa silage (Medicago sativa) in organic pig production[J].Livestock Science, 2017, 200:46-52.
[11] CHEN L, GAO L X, ZHANG H F.Effect of graded levels of fiber from alfalfa meal on nutrient digestibility and flow of fattening pigs[J].Journal of Integrative Agriculture, 2014, 13(8):1746-1752.
[12] 杨海天.饲粮纤维水平对不同遗传基础育肥猪生长性能及肉品质的影响[D].长春:吉林农业大学, 2019. YANG H T.Effects of dietary fiber levels on growth performance and meat quality of different genetically based finishing pigs[D].Changchun:Jilin Agricultural University, 2019.(In Chinese)
[13] LI J, ZHANG J, YANG Y, et al.Comparative characterization of lipids and volatile compounds of Beijing Heiliu and Laiwu Chinese Black pork as markers[J].Food Research International, 2021, 146(11):110433.
[14] TU T, TANG X Y, GE Q Q, et al.Screening out important substances for distinguishing Chinese indigenous pork and hybrid pork and identifying different pork muscles by analyzing the fatty acid and nucleotide contents[J].Food Chemistry, 2021, 350:129219.
[15] 古丽娜尔·毛肯.牧草颗粒饲料与干草粉加工利用及应用前景[J].畜牧兽医科学, 2021, 3:130-131. GULINAER M K.Processing, utilization and application prospect of forage pellet feed and hay powder[J].Graziery Veterinary Sciences:Electronic Version, 2021, 3:130-131.(in Chinese)
[16] VAREL V H, ROBINSON I M, JUNG H J.Influence of dietary fiber on xylanolytic and cellulolytic bacteria of adult pigs[J].Applied and Environmental Microbiology, 1987, 53(1):22-26.
[17] KASS M L, VAN S, POND W G.Utilization of dietary fiber from alfalfa by growing swine.II.Volatile fatty acid concentrations in and disappearance from the gastrointestinal tract[J].Journal of Animal Science, 1980, 50(1):192-197.
[18] WONG J, SOUZA R D, KENDALL C, et al.Colonic health:Fermentation and short chain fatty acids[J].Journal of Clinical Gastroenterology, 2006, 40(3):235-243.
[19] BARBARA W, LUCAS G, MICHAEL G, et al.Gut fermentation of dietary fibres:Physico-chemistry of plant cell walls and implications for health[J].International Journal of Molecular Sciences, 2017, 18(10):2203.
[20] 余凡, 葛亚龙, 杨恒拓.苜蓿中膳食纤维的提取工艺及生理功能的研究进展[J].杭州化工, 2013, 43(4):8-10. YU F, GE Y L.YANG H T.Research progress on the extraction technology and physiological function of dietary fiber in alfalfa[J].Hangzhou Chemical Industry, 2013, 43(4):8-10.(in Chinese)
[21] 王嘉为, 张蕾, 祝皎月, 等.饲粮中添加苜蓿草粉对生长猪结肠微生物区系及其代谢产物的影响[J].动物营养学报, 2016, 28(9):2715-2723. WANG J W, ZHANG L, ZHU J Y, et al.Effects of dietary alfalfa meal supplementation on colonic microbiota and its metabolites in growing pigs[J].Chinese Journal of Animal Nutrition, 2016, 28(9):2715-2723.(in Chinese)
[22] 李润林, 王占彬, 杜玉杰, 等.紫花苜蓿的营养特性及在猪生产中的应用研究进展[J].饲料博览, 2018, 5:19-25. LI R L, WANG Z B, DU Y J, et al.Research progress on the nutritional characteristics of alfalfa and application in pig production[J].Feed Review, 2018, 5:19-25.(in Chinese)
[23] DANIELSON D M, BUTCHER J E, STREET J C.Estimation of alfalfa pasture intake and nutrient utilization by growing-finishing swine[J].Journal of Animal Science, 1969, 28(1):6.
[24] 孙健.不同添加水平的紫花苜蓿草粉对育肥猪生长性能、养分表观消化率的影响[J].中国饲料, 2019, 13:75-77. SUN J.Effects of different levels of alfalfa meal on growth performance and nutrient digestibility of finishing pigs[J].China Feed, 2019, 13:75-77.(in Chinese)
[25] 李碧侠, 赵为民, 付言峰, 等.苜蓿草粉对苏山猪生长速度、血液生化指标及屠宰性能的影响[J].西南农业学报, 2020, 33(11):2675-2680. LI B X, ZHAO W M, FU Y F, et al.Effects of alfalfa meal on growth rate, blood biochemical indexes and slaughter performance of Sushan pigs[J].Southwest China Journal of Agricultural Sciences, 2020, 33(11):2675-2680.(in Chinese)
[26] 王彦华, 程宁宁, 郑爱荣, 等.苜蓿草粉和苜蓿皂苷对肥育猪生长性能和抗氧化性能的影响[J].动物营养学报, 2013, 25(12):2981-2988. WANG Y H, CHENG N N, ZHENG A R, et al.Effects of alfalfa meal and alfalfa saponin on growth performance and antioxidant function of finishing pigs[J].Chinese Journal of Animal Nutrition, 2013, 25(12):2981-2988.(in Chinese)
[27] 黄新.苜蓿草粉在杜洛克生长肥育猪生产上的应用研究[D].杭州:浙江大学, 2005. HUANG X, The application study of the alfalfa meals in the production of growing-finishing Duroc[D].Hangzhou:Zhejiang University, 2005.(in Chinese)
[28] 方秋红, 侯永清, 赵迪, 等.植物精油对断奶仔猪生长性能及血液生化指标的影响[J].饲料工业, 2014, 35(17):44-47. FANG Q H, HOU Y Q, ZHAO D, et al.Effects of essential oil on growth performance and blood biochemical parametersin weaned piglets[J].Feed Industry, 2014, 35(17):44-47.(in Chinese)
[29] 朱彦宾, 兰天漪, 彭阳洋, 等.不同饲养模式下藏猪屠宰性能、肉质及血清生化指标的比较研究[J].黑龙江畜牧兽医, 2021, 11:75-79. ZHU Y B, LAN T Y, PENG Y Y, et al.Comparative study on slaughtering performance, meat quality and blood biochemical indexes of Tibetan swine in different feeding modes[J].Heilongjiang Animal Science and Veterinary Medicine, 2021, 11:75-79.(in Chinese)
[30] 霍永久, 占今舜, 余同水, 等.饲粮不同蛋白水平对淮猪生长性能、肉品质和血清生化指标的影响[J].草业学报, 2015, 24(6):133-141. HUO Y J, ZHAN J S, YU T S, et al.The effect of diet with different crude protein levels on growth performance, meat quality and serum parameters in Huai pigs[J].Acta Prataculturae Sinica, 2015, 24(6):133-141.(in Chinese)
[31] 郑心力, 孙瑞萍, 魏立民, 等.饲粮蛋白质水平对海南猪生产性能和血液生化指标的影响[J].中国畜牧兽医, 2013, 40(7):93-96. ZHENG X L, SUN R P, WEI L M, et al.Effect of the level of protein in dietary on growth performance and blood biochemical indices in Hainan pigs[J].China Animal Husbandry & Veterinary Medicine, 2013, 40(7):93-96.(in Chinese)
[32] 邓培华, 王娟, 洼桑措.中草药添加剂对断奶仔猪生长性能、养分表观消化率、血清生化指标和免疫指标的影响[J].饲料研究, 2021, 44(10):17-21. DENG P H, WANG J, WA S C.Effect of Chinese herbal medicine additives on growth performance, nutrient apparent digestibility, serum biochemical index and immune index of weaned piglets[J].Feed Research, 2021, 44(10):17-21.(in Chinese)
[33] 唐武, 蔡巧利, 蒋慧姣, 等.饲粮添加益生菌和合生元对环江香猪生长性能及血浆生化参数的影响[J].动物营养学报, 2021, 33(1):546-552. TANG W, CAI Q L, JIANG H J, et al.Effects of dietary supplementation with probiotics and synbiotics on growth performance and plasma biochemical parameters of Huanjiang mini-piglets[J].Chinese Journal of Animal Nutrition, 2021, 33(1):546-552.(in Chinese)
[34] 安亚辉, 曹洪战, 苗玉涛, 等.饲粮粗纤维水平对生长育肥期深县猪生长性能、营养物质表观消化率和血清生化指标的影响[J].动物营养学报, 2019, 31(9):4371-4377. AN Y H, CAO H Z, MIAO Y T, et al.Effects of dietary crude fiber level on growth performance, nutrient apparent digestibility and serum biochemical indexes of growing and finishing Shenxian pigs[J].Chinese Journal of Animal Nutrition, 2019, 31(9):4371-4377.(in Chinese)
[35] NINA L G, MICHEL B, TOBIAS C W, et al.Lipid droplets and liver disease:From basic biology to clinical implications[J].Nature Reviews Gastroenterology & Hepatology, 2017, 14:343.
[36] 谢前进, 缪晓兰, 付少军.联合检测血清总胆汁酸酶类在肝脏损害诊断中的临床意义[J].检验医学与临床, 2011, 8(18):2232-2233. XIE Q J, MIAO X L, FU S J.The clinical significance of combined detection of serum total bile acid enzymes in the diagnosis of liver damage[J].Laboratory Medicine and Clinic, 2011, 8(18):2232-2233.(in Chinese)
[37] LIU Y Y, KONG X F, JIANG G L, et al.Effects of dietary protein/energy ratio on growth performance, carcass trait, meat quality, and plasma metabolites in pigs of different genotypes[J].Journal of Animal Science and Biotechnology, 2015, 6(4):435-444.
[38] 朱明霞, 刘文强, 侯国生.鲁西地方黑猪血液生化指标与肉量和肉质的相关性研究[J].畜牧与兽医, 2013, 45(8):62-66. ZHU M X, LIU W Q, HOU G S.Study on the correlation between blood biochemical indexes and meat quantity and quality of Luxi local Black pigs[J].Animal Husbandry & Veterinary Medicine, 2013, 45(8):62-66.(in Chinese)
[39] SERENA A, JØRGENSEN H, BACH KNUDSEN K E.Absorption of carbohydrate-derived nutrients in sows as influenced by types and contents of dietary fiber[J].Journal of Animal Science, 2009, 87(1):136-147.
[40] 廖日滔, 郭静科, 李冰洁, 等.自由基相关细胞信号传导的研究进展[J].中国细胞生物学学报, 2014, 36(11):1573-1583. LIAO R T, GUO J K, LI B J, et al.The review on the cell signal transduction pathway related to free radicals[J].Chinese Journal of Cell Biology, 2014, 36(11):1573-1583.(in Chinese)
[41] 王璐, 海春旭, 秦绪军.VA与VE对辐射诱发大鼠过氧化损伤的保护作用[J].解放军预防医学杂志, 2001, 2:98-100. WANG L, HAI C X, QIN X J.Peotective effect of vitamin A and vitamin E on peroxidative damage induced by radiation in rats[J].Journal of Preventive Medicine of Chinese People's Liberation Army, 2001, 2:98-100.(in Chinese)
[42] 王彦华, 何云, 郑爱荣, 等.苜蓿草粉和苜蓿皂苷对肥育猪生产性能和抗氧化性能的影响[A].第五届中国苜蓿发展大会论文集[C].2013. WANG Y H, HE Y, ZHENG A R, et al.Effects of alfalfa meal and alfalfa saponins on the production performance and antioxidant performance of finishing pigs[A].Proceedings of the Fifth China Alfalfa Development Conference[C].2013.(in Chinese)
[43] 史莹华, 王成章, 徐兵, 等.苜蓿皂甙对断奶仔猪生长性能和抗氧化性能的影响[J].草地学报, 2010, 18(5):735-739. SHI Y H, WANG C Z, XU B, et al.Effects of alfalfa saponins on growth performance and antioxidant properties of weaned piglets[J].Acta Agrestia Sinica, 2010, 18(5):735-739.(in Chinese)
[44] HWANG J, HODIS H N, SEVANIAN A.Soy and alfalfa phytoestrogen extracts become potent low-density lipoprotein antioxidants in the presence of acerola cherry extract[J].Journal of Agricultural and Food Chemistry, 2001, 49(1):308-314.
[45] 李苗苗, 靳思玉, 牛志力, 等.苜蓿黄酮的生物活性功能及其在反刍动物中的应用[J].中国饲料, 2019, 13:4-8. LI M M, JIN S Y, NIU Z L, et al.Bioactive function of flavonoids from alfalfa and its application in ruminants[J].China Feed, 2019, 13:4-8.(in Chinese)
[46] 葛亚龙, 杨恒拓, 余凡, 等.苜蓿多糖的提取及其清除羟基自由基作用研究[J].食品工业, 2014, 35(1):7-10. GE Y L, YANG H T, YU F, et al.Optimization of microwave extraction technology and scavenging effect for hydroxyl radical of the polysaccharide from Medicago sativa Linn[J].The Food Industry, 2014, 35(1):7-10.(in Chinese)
[47] 李敬双, 刘英姿, 唐雨顺, 等.苜蓿多糖对小鼠淋巴细胞增殖和NK细胞活性影响的研究[J].中国农学通报, 2012, 28(32):89-93. LI J S, LIU Y Z, TANG Y S, et al.Research of the effect of alfalfa polysaccharides on mice spleen lymphocytes proliferation and NK cell activity[J].Chinese Agricultural Science Bulletin, 2012, 28(32):89-93.(in Chinese)
[48] 张世军, 王三虎, 赵坤.苜蓿多糖对猪瘟兔化弱毒疫苗免疫应答强化作用的研究[J].河南农业科学, 2003, 10:57-60. ZHANG S J, WANG S H, ZHAO K.Studies on enhaning effect of alfalfa polysaccharide on the immune response of lapinized Chinese strain of classical Swine fever virus[J].Journal of Henan Agricultural Sciences, 2003, 10:57-60.(in Chinese)
[49] 李晓, 王婷婷, 廖智慧, 等.紫花苜蓿粉对仔猪生长性能和血清免疫指标的影响[J].黑龙江畜牧兽医, 2018, 16:81-83. LI X, WANG T T, LIAO Z H, et al.Effect of alfalfa meal on growth performance and serum immune indexes of piglets[J].Heilongjiang Animal Science and Veterinary Medicine, 2018, 16:81-83.(in Chinese)