《中国畜牧兽医》 ›› 2017, Vol. 44 ›› Issue (1): 123-130.doi: 10.16431/j.cnki.1671-7236.2017.01.017

• 遗传繁育 • 上一篇    下一篇

ESR、OPNRBP4基因多态性对大白猪繁殖性状的影响

刘乙1, 马力鹏1, 李涛1, 李梦寻1, 沈永巧1, 黄涛1, 胡九英2, 汪德明2, 王忻2, 来红霞2   

  1. 1. 石河子大学动物科技学院, 石河子 832000;
    2. 新疆正大食品有限公司, 五家渠 831301
  • 收稿日期:2016-07-11 出版日期:2017-01-20 发布日期:2017-01-19
  • 通讯作者: 黄涛 E-mail:taohuang100@sina.com
  • 作者简介:刘乙(1991-),女,四川内江人,硕士生,研究方向:动物遗传育种与繁殖,E-mail:liuyi_shz@163.com
  • 基金资助:

    国家国际科技合作项目(2014DFA31840);国家自然科学基金(31460586);教育部留学归国人员科研启动基金((2013)1092);石河子大学动植物育种专项(gxjs2013-yz06)

Influence of ESR, OPN and RBP4 Genes Polymorphisms on Reproductive Traits of Yorkshire Sows

LIU Yi1, MA Li-peng1, LI Tao1, LI Meng-xun1, SHENG Yong-qiao1, HUANG Tao1, HU Jiu-ying2, WANG De-ming2, WANG Xin2, LAI Hong-xia2   

  1. 1. College of Animal Science and Technology, Shihezi University, Shihezi 832000, China;
    2. Xinjiang Chia Tai Food Co., Ltd., Wujiaqu 831301, China
  • Received:2016-07-11 Online:2017-01-20 Published:2017-01-19

摘要:

为了探讨雌激素受体(estrogen receptor,ESR)、骨桥蛋白(osteopontin,OPN)和视黄醇结合蛋白4(retinol binding proteins 4,RBP4)的多态性对大白猪繁殖性状的影响,本研究采用PCR-RFLP技术检测试验猪群ESR、OPNRBP4基因的多态性,并分析其对大白猪总产仔数、产活仔数、健仔数、窝重、初生重、死胎、木乃伊、弱仔数和畸形数等性状的影响。结果显示,在大白经产母猪中,ESROPN基因的优势基因为A等位基因,RBP4基因的优势基因为B等位基因;ESR基因AB与BB基因型在总产仔数、产活仔数性状上均高于AA基因型;OPN基因在总产仔数性状上AA基因型最高,在产活仔数、健仔数、窝重性状上均为BB > AA > AB;RBP4基因在总产仔数、产活仔数、健仔数、窝重性状上均为AA > BB > AB;合并基因型分析发现ESR-RBP4最优的分子标记组合为ABAA;OPN-RBP4最优的分子标记组合为BBAA。因此,ESR、OPNRBP4基因多态性与大白猪繁殖性状相关。

关键词: ESR基因; OPN基因; RBP4基因; 母猪; 繁殖性状

Abstract:

To study the influence of estrogen receptor (ESR), osteopontin (OPN) and retinol binding proteins 4 (RBP4) genotypes on reproductive performance of Yorkshire sows, PCR-RLFP technique was used for genotyping, and the effects of genotypes on reproductive traits were analyzed. The results showed that A allele of ESR and OPN genes were predominant allele, and B allele of RBP4 gene was predominant allele. TNB,NBA of ESR gene AB and BB genotypes were higher than AA genotype;TNB of OPN gene AA genotype was the highest, but the NBA, NHB and WB for OPN gene genotypes were BB > AA > AB;The TNB,NBA,NHB and WB for RBP4 gene genotypes were AA > BB > AB. Then combing genotype analysis showed that ABAA of ESR-RBP4 and BBAA of OPN-RBP4 were the most prolific alleles, respectively. The present data revealed that ESR, OPN and RBP4 genes polymorphisms were associated with reproductive performance of Yorkshire sows.

Key words: ESR gene; OPN gene; RBP4 gene; sow; reproductive performance

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