ADCY3和IGF1基因结构变异与香猪繁殖性状的关联分析

doi:10.16431/j.cnki.1671-7236.2021.08.024

中国畜牧兽医 ›› 2021, Vol. 48 ›› Issue (8): 2920-2927.doi: 10.16431/j.cnki.1671-7236.2021.08.024

ADCY3和IGF1基因结构变异与香猪繁殖性状的关联分析

陈芳^1,2, 孙镘熹^1,2, 王志勇^1,2, 刘畅³, 黄世会⁴, 王嘉福^1,2, 冉雪琴^1,4, 牛熙^1,2

1. 贵州大学生命科学学院, 贵阳 550025;
2. 贵州大学农业生物工程研究院, 贵阳 550025;
3. 贵州中医药大学药学院, 贵阳 550025;
4. 贵州大学动物科学学院, 贵阳 550025

收稿日期:2021-03-09 出版日期:2021-08-20 发布日期:2021-08-19
通讯作者: 王嘉福, 冉雪琴 E-mail:jfwang@gzu.edu.cn;xqran@gzu.edu.cn
作者简介:陈芳(1994-),女,贵州贵阳人,硕士生,研究方向:生物工程,E-mail:3634557339@qq.com
基金资助:
国家高技术研究发展计划（863计划）课题（2013AA102503）；国家自然科学基金（31960641、31672390）；贵州省"百"层次创新型人才培养项目（黔科合人才（2016）4012号）；贵州省主要畜禽基因组大数据分析与应用研究科技创新人才团队（黔科合平台人才[2019]5615）；贵州省科技支撑计划（黔科合支撑[2017]2585、黔科合支撑[2017]2587）；贵州省科技计划项目（黔科合LH字[2017]7273号）

Association Analysis of Structural Variation of ADCY3 and IGF1 Genes with Reproductive Traits in Xiang Pigs

CHEN Fang^1,2, SUN Manxi^1,2, WANG Zhiyong^1,2, LIU Chang³, HUANG Shihui⁴, WANG Jiafu^1,2, RAN Xueqin^1,4, NIU Xi^1,2

1. College of Life Sciences, Guizhou University, Guiyang 550025, China;
2. Institute of Agro-Bioengineering, Guizhou University, Guiyang 550025, China;
3. Guizhou University of Traditional Chinese Medicine School of Pharmacy, Guiyang 550025, China;
4. College of Animal Science, Guizhou University, Guiyang 550025, China

Received:2021-03-09 Online:2021-08-20 Published:2021-08-19

摘要/Abstract

摘要： 试验旨在研究卵巢类固醇生成信号通路中腺苷酸环化酶3（adenylatecyclase 3，ADCY3）、胰岛素样生长因子1（insulin like growth factor 1，IGF1）基因结构变异的遗传多样性及其与香猪母猪繁殖性状的相关性。采用PCR技术对269头香猪进行ADCY3和IGF1基因结构变异研究，利用在线软件计算遗传杂合度（He）、遗传纯合度（Ho）、有效等位基因数（Ne）及多态信息含量（PIC），并利用单因素方差分析、LSD法分析不同基因型与初产和经产香猪的总产仔数、产活仔数、初生重及乳头数等繁殖性状的关联性。结果显示，2个结构变异ADCY3-I₁-sv506和IGF1-I₃-sv302在香猪群体中均存在多态性，均有3种基因型：DD、DI和II，2种等位基因：D和I。其中，ADCY3-I₁-sv506中DI基因型为优势基因型（0.569），D为优势等位基因（0.601）；IGF1-I₃-sv302中II基因型为优势基因型（0.717），I为优势等位基因（0.816）。ADCY3-I₁-sv506和IGF1-I₃-sv302多态信息含量（PIC）均为中度多态（0.25<PIC<0.5），杂合度分别为0.479和0.300。关联性分析结果发现，ADCY3-I₁-sv506位点DD基因型个体的初产和经产母猪总产仔数显著高于II基因型个体（P<0.05）；IGF1-I₃-sv302位点II基因型个体的初产、经产母猪总产仔数和经产母猪产活仔数均显著高于DI基因型型个体（P<0.05）。结果表明，2个结构变异位点ADCY3-I₁-sv506和IGF1-I₃-sv302与香猪母猪的繁殖性能有直接关联，可作为香猪母猪繁殖性能选育的候选分子标记。

关键词: 香猪; ADCY3基因; IGF1基因; 结构变异; 繁殖性状

Abstract: The aim of this study was to investigate the genetic diversity of structural variation (SV) of adenylatecyclase 3 (ADCY3) and insulin like growth factor 1 (IGF1) genes in the ovarian steroidogenesis signaling pathway and their correlation with reproductive traits in Xiang pig sows.The SV of ADCY3 and IGF1 genes was studied in 269 Xiang pigs by PCR technique, the genetic heterozygosity (He), genetic purity(Ho), effective allele number(Ne) and polymorphic information content (PIC) were calculated using online software, and the association of different genotypes with reproductive traits such as total litter size, live litter size, newborn weight and number of teats in primiparous and multiparity Xiang pigs were analyzed using One-Way ANOVA and LSD method.The results showed that two SVs ADCY3-I₁-sv506 and IGF1-I₃-sv302 contained plenty of polymorphisms in Xiang pigs population, there were three genotypes of DD, DI and II, and two alleles of D and I.DI genotype (0.569) and D allele (0.601) were dominant in ADCY3-I₁-sv506, II genotype (0.717) and I allele (0.816) were dominant in IGF1-I₃-sv302.ADCY3-I₁-sv506 and IGF1-I₃-sv302 were moderate polymorphism (0.25<PIC<0.5), the heterozygosity was 0.479 and 0.300, respectively.The correlation analysis results showed that the total number born of primiparous and multiparous sows of DD genotype in ADCY3-I₁-sv506 were significantly higher than that of II genotype (P<0.05), the total number born of primipara and multiparous sows and the number born alive of multiparous sows of II genotype in IGF1-I₃-sv302 was significantly higher than that of DI genotype(P<0.05).In conclusion, both of two SVs in of ADCY3 and IGF1 genes had correlation ship with the reproductive performance of Xiang pigs, it could be taken as the candidate molecular markers for breeding of Xiang pigs sows.

Key words: Xiang pigs; ADCY3 gene; IGF1 gene; structural variation; reproductive traits

中图分类号:

S813.3

陈芳, 孙镘熹, 王志勇, 刘畅, 黄世会, 王嘉福, 冉雪琴, 牛熙. ADCY3和IGF1基因结构变异与香猪繁殖性状的关联分析[J]. 中国畜牧兽医, 2021, 48(8): 2920-2927.

CHEN Fang, SUN Manxi, WANG Zhiyong, LIU Chang, HUANG Shihui, WANG Jiafu, RAN Xueqin, NIU Xi. Association Analysis of Structural Variation of ADCY3 and IGF1 Genes with Reproductive Traits in Xiang Pigs[J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(8): 2920-2927.

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ADCY3和IGF1基因结构变异与香猪繁殖性状的关联分析

Association Analysis of Structural Variation of ADCY3 and IGF1 Genes with Reproductive Traits in Xiang Pigs

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