调控香猪繁殖候选miRNA筛选

doi:10.16431/j.cnki.1671-7236.2021.09.002

中国畜牧兽医 ›› 2021, Vol. 48 ›› Issue (9): 3118-3127.doi: 10.16431/j.cnki.1671-7236.2021.09.002

调控香猪繁殖候选miRNA筛选

禄欢¹, 牛熙¹, 黄娅丽¹, 李升¹, 冉雪琴², 黄世会², 王嘉福¹

1. 贵州大学农业生物工程研究院, 贵阳 550025;
2. 贵州大学动物科学学院, 贵阳 550025

收稿日期:2021-03-21 出版日期:2021-09-20 发布日期:2021-09-17
通讯作者: 王嘉福 E-mail:jfwang@gzu.edu.cn
作者简介:禄欢(1995-),女,贵州毕节人,硕士生,研究方向:生物化学与分子生物学,E-mail:1724540580@qq.com
基金资助:
国家高技术研究发展计划（863计划）课题（2013AA102503）；国家自然科学基金（31960641、31672390）；贵州省"百"层次创新型人才培养项目（黔科合人才（2016）4012号）；贵州省主要畜禽基因组大数据分析与应用研究科技创新人才团队（黔科合平台人才[2019]5615）；贵州省科技支撑计划（黔科合支撑[2017]2585、[2017]2587）；贵州省科技计划项目（黔科合LH字[2017]7273号）；贵州大学培育项目（黔科合平台人才[2018]5781号）

Screening of Candidate miRNA for Regulating Xiang Pig Reproduction

LU Huan¹, NIU Xi¹, HUANG Yali¹, LI Sheng¹, RAN Xueqin², HUANG Shihui², WANG Jiafu¹

1. Institute of Agricultural Biological Engineering, Guizhou University, Guiyang 550025, China;
2. College of Animal Science, Guizhou University, Guiyang 550025, China

Received:2021-03-21 Online:2021-09-20 Published:2021-09-17

摘要/Abstract

摘要： 研究旨在从香猪卵巢small RNA测序数据中挖掘调控香猪繁殖的microRNA （miRNA），解析miRNA调控香猪卵巢功能及繁殖性状的分子机制，对于猪的遗传选育具有重要意义。研究选取发情期和间情期的香猪各4头，屠宰后取其卵巢组织，提取总RNA进行small RNA测序，利用生物信息学方法检测miRNA表达谱，筛选差异表达的miRNA，预测差异表达miRNA靶基因，并对靶基因进行GO功能富集和KEGG通路富集分析。结果显示，香猪卵巢组织中miRNA在染色体上呈不均匀分布，主要分布于1号染色体和X染色体上。香猪卵巢中共有627个已知猪miRNAs表达，其中有34个差异表达miRNAs，表达量前五的分别是miR-23、let-7i-5p、miR-103、miR-30e-5p和miR-1271-5p。GO功能分析结果显示，靶基因主要参与的生物学过程是细胞过程（cellular process），主要分布于细胞（cell），主要分子功能是结合（binding）；KEGG显著富集通路中，促性腺激素释放激素受体通路（gonadotropin-releasing hormone receptor pathway）、胰岛素样生长因子通路（IGF pathway）和表皮生长因子受体信号通路（EGF receptor signaling pathway）与卵母细胞的发育成熟相关，因此推测miR-23b、let-7i-5p、miR-103、miR-30e-5p和miR-1271-5p可能参与了香猪繁殖调控。本研究初步筛选出5个可能调控香猪繁殖性能的miRNAs，可为从分子水平提高香猪产仔数提供理论基础。

关键词: miRNA; 卵巢; 靶基因; 繁殖力; 香猪

Abstract: This study was aimed to excavate the microRNAs that regulate the reproduction of Xiang pigs from the small RNA sequencing data of Xiang pigs ovaries, it is of great significance fo analyze the molecular mechanism of miRNAs regulating ovarian function and reproductive traits in pig. In this study, four estrus pigs and four diestrus pigs were used as the test animals. The ovary tissues were collected from Xiang pigs after slaughter. The total RNA was extracted from ovary tissues for small RNA sequencing, the profile of miRNAs expression were calculated with bioinformatics selection, and the different expression target genes were predicted and the GO function enrichment and KEGG pathway enrichment analysis of the target genes were performed. The results showed that miRNAs were no uniformly distributed on chromosomes, mainly on chromosome 1 and chromosome X. Total of 627 known porcine miRNAs were expressed in the ovaries of Xiang pigs, of which 34 were differently expressed miRNAs. The top five expression levels of miRNAs were miR-23, let-7i-5p, miR-103, miR-30E-5p and miR-1271-5p. The results of GO function analysis showed that the main biological processes involved in target genes were cellular processes mainly distributed in cells and the main molecular function was binding. KEGG pathway analysis significantly enriched gonadotropin-releasing hormone receptor pathway, insulin-like growth factor pathway and EGF receptor signaling pathway, which were associated with oocyte development and maturation. Therefore, it was speculated that miR-23b, let-7i-5p, miR-103, miR-30e-5p and miR-1271-5p could be used as candidate miRNAs affecting the reproductive regulation of Xiang pigs. In this study, five miRNA molecules that might regulate the reproduction of Xiang pigs were preliminarily screened, which could provide a theoretical basis for improving the litter size of Xiang pigs at molecular level.

Key words: miRNA; ovary; target gene; fertility; Xiang pigs

中图分类号:

S828

禄欢, 牛熙, 黄娅丽, 李升, 冉雪琴, 黄世会, 王嘉福. 调控香猪繁殖候选miRNA筛选[J]. 中国畜牧兽医, 2021, 48(9): 3118-3127.

LU Huan, NIU Xi, HUANG Yali, LI Sheng, RAN Xueqin, HUANG Shihui, WANG Jiafu. Screening of Candidate miRNA for Regulating Xiang Pig Reproduction[J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(9): 3118-3127.

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调控香猪繁殖候选miRNA筛选

Screening of Candidate miRNA for Regulating Xiang Pig Reproduction

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