水牛瘦素基因单核苷酸多态性分析

doi:10.16431/j.cnki.1671-7236.2016.09.029

《中国畜牧兽医》 ›› 2016, Vol. 43 ›› Issue (9): 2418-2424.doi: 10.16431/j.cnki.1671-7236.2016.09.029

水牛瘦素基因单核苷酸多态性分析

庞春英, 邓廷贤, 陆杏蓉, 朱鹏, 段安琴, 梁贤威

中国农业科学院广西水牛研究所, 农业部(广西)水牛遗传繁育重点实验室, 南宁 530001

收稿日期:2016-01-28 出版日期:2016-09-20 发布日期:2016-09-20
通讯作者: 梁贤威 E-mail:liangbri@126.com
作者简介:庞春英(1972-),女,广西博白人,学士,副研究员,研究方向:动物遗传育种与繁殖,E-mail:pangcy800@163.com
基金资助:
广西科技攻关国际交流与合作项目（桂科合14123001-5）；广西水产畜牧兽医局科技项目（桂渔牧科201452032）；广西自然科学基金重点项目（2014GXNSFDA118017）

Single Nuclease Polymorphism Analysis of Leptin Gene in Buffalo

PANG Chun-ying, DENG Ting-xian, LU Xing-rong, ZHU Peng, DUAN An-qin, LIANG Xian-wei

Guangxi Provincial Key Laboratory of Buffalo Genetics, Breeding and Reproduction Technology, Buffalo Research Institute, Chinese Academy of Agricultural Sciences, Nanning 530001, China

Received:2016-01-28 Online:2016-09-20 Published:2016-09-20

摘要/Abstract

摘要：

试验旨在检测水牛瘦素（Leptin）基因序列的多态性，为进一步开展水牛标记辅助育种研究奠定基础。运用DNA混合池结合直接测序及高分辨熔解曲线（HRM）法在182头奶水牛个体中进行Leptin基因SNP位点的筛选和分型。结果表明，在试验群体中Leptin基因共发现7个SNPs，位于内含子1、内含子3和外显子3区域，分别是A3123G、A3776G、A4154G、A5228G、A5524C、G5573T和A5751C。除了SNP5和SNP6位点在尼里－拉菲水牛群体中的多态信息含量（PIC）属于低度多态之外，所有SNPs位点在3个水牛群体中均属于中度多态。经χ²检验，所有SNPs位点的突变在尼里－拉菲水牛群体均达到Hardy-Weinberg平衡状态（P>0.05）。本研究成功筛查7个水牛Leptin基因SNPs位点并进行了基因分型，为下一步开展奶水牛Leptin基因的标记－性状关联分析奠定基础。

关键词: 水牛; 瘦素基因; 多态性; 基因分型

Abstract:

This study was aimed to detect the polymorphisms of Leptin gene in buffalo that provided a fundamental for further study on marker assisted breeding in buffaloes.The single nuclease polymorphisms (SNP) of Leptin gene were identified and genotyped by using DNA pooled sequencing and high-resolution melting (HRM) method in three buffalo breeds with 182 buffalo individuals,respectively.The results showed that seven SNPs of Leptin gene were identified in studied population that were located in the intron 1,intron 2 and exon 3 regions,respectively.All the SNPs loci were moderate polymorphism except for the SNP5 and SNP6.The χ²-test indicated that all the SNPs loci were in agreement with Hardy-Weinberg equilibrium in Nili-Ravi population (P>0.05).Our findings revealed that seven SNPs of Leptin gene in buffalo were identified and genotyped in population,which provide the data support for further analyzing the associations between these polymorphism and production traits in buffaloes.

Key words: buffalo; Leptin gene; polymorphism; genotype

中图分类号:

S813.3

庞春英, 邓廷贤, 陆杏蓉, 朱鹏, 段安琴, 梁贤威. 水牛瘦素基因单核苷酸多态性分析[J]. 《中国畜牧兽医》, 2016, 43(9): 2418-2424.

PANG Chun-ying, DENG Ting-xian, LU Xing-rong, ZHU Peng, DUAN An-qin, LIANG Xian-wei. Single Nuclease Polymorphism Analysis of Leptin Gene in Buffalo[J]. , 2016, 43(9): 2418-2424.

参考文献

[1] van der Lende T,Te Pas M F,Veerkamp R F,et al.Leptin gene polymorphisms and their phenotypic associations[J].Vitam Horm,2005,71:373-404.
[2] Nkrumah J D,Li C,Yu J,et al.Polymorphisms in the bovine leptin promoter associated with serum leptin concentration,growth,feed intake,feeding behavior,and measures of carcass merit[J].J Anim Sci,2005,83(1):20-28.
[3] Zieba D A,Amstalden M,Williams G L.Regulatory roles of leptin in reproduction and metabolism:A comparative review[J].Domest Anim Endocrinol,2005,29(1):166-185.
[4] Konfortov B A,Licence V E,Miller J R.Re-sequencing of DNA from a diverse panel of cattle reveals a high level of polymorphism in both intron and exon[J].Mamm Genome,1999,10(12):1142-1145.
[5] Yoon D H,Cho B H,Park B L,et al.Highly polymorphism bovine Leptin gene[J].Asian-australas J Anim Sci,2005,18(11):1548-1551.
[6] Buchanan F C,Fizsimmons C J,van Kessel A G,et al.A sense mutation in the bovine Leptin gene affects fat content and mRNA levels[J]. Genet Sel Evol,2002,34:105-106.
[7] Komisarek J.Impact of LEP and LEPR gene polymorphisms on functional traits in polish Holstein-Friesian cattle[J].Anim Sci Papers Rep,2010,28:133-141.
[8] Giblin L,Butler S T,Kearney B M,et al.Association of bovine leptin polymorphisms with energy output and energy storage traits in progeny tested Holstein-Friesian dairy cattle sires[J].BMC Genet,2011,11:73.
[9] Tanpure T,Dubey P K,Singh K P.PCR-SSCP analysis of leptin gene and its association with milk production traits in river buffalo (Bubalus bubalis)[J].Trop Anim Health Prod,2012,44(7):1587-1592.
[10] 刘文博,陈思睿,郑江霞,等.利用混合样本池法对鸡显性白羽基因PMEL17突变位点的检测[J].遗传,2010,32(2):148-152.
[11] Foulkes A S.Applied statistical genetics with R:For population-based association studies[M].New York:Springer Verlag,2009.
[12] Vallinoto M,Schneider M P,Silva A,et al.Molecular cloning and analysis of the swamp and river buffalo Leptin gene[J].Anim Genet,2004,35(6):462-463.
[13] 王悦冰,郎志宏,黄大昉.内含子对真核基因表达调控的影响[J].生物技术通报,2008,195(4):1-4.
[14] 张开慧.内含子的功能与应用[J].中国畜牧兽医,2012,39(7):80-84.
[15] Sontheimer E J.Bridging sulfur substitutions in the analysis of pre-RNA splicing[J].Methods,1999,18(1):29-37.
[16] 乔海云,赵倩君,姚娜,等.绵羊H-FABP基因单核苷酸多态性的研究[J].遗传,2009,31(7):725-731.
[17] 吴丽丽,赵晓枫,陈哲,等.湖羊瘦素基因内含子2多态性及其与体重性状的相关性分析[J].中国畜牧兽医,2012,39(2):114-116.
[18] Liefers S C,Te Pas M F,Veerkamp R F,et al.Association between Leptin gene polymorphisms and production,live weight,energy balance,feed intake,and fertility in Holstein heifers[J]. J Dairy Sci,2002,85(6):1633-1638.
[19] Trakovicka A,Moravcikova N,Kasarda R.Genetic polymorphisms of Leptin and Leptin receptor genes in relation with production and reproduction traits in cattle[J].Acta Biochim Pol,2013,60(4):783-787.
[20] Zetouni L,de Camargo G M,da Silva Fonseca P D,et al.Effects of a single nucleotide polymorphism in the Leptin gene on the productive traits of dairy buffaloes (Bubalus bubalis)[J].Mol Biol Rep,2013,40(8):5159-5163.
[21] Hajihosseinlo A,Jafari S,Ajdary M.The relationship of GH and LEP gene polymorphisms with fat-tail measurements (fat-tail dimensions) in fat-tailed Makooei breed of Iranian sheep[J].Adv Biomed Res,2015,4:172.

水牛瘦素基因单核苷酸多态性分析

Single Nuclease Polymorphism Analysis of Leptin Gene in Buffalo

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

[1]	徐媛媛, 郑海英, 杨春艳, 邓廷贤, 黄晨茜, 冯超, 陆杏蓉, 段安琴, 莫霞, 马小娅, 尚江华. 抑制BMP/Smad信号通路对水牛卵巢颗粒细胞生长和类固醇激素合成的影响[J]. 中国畜牧兽医, 2023, 50(6): 2354-2362.
[2]	肖鹏, 尚江华, 杨春艳, 李孟琪, 段安琴, 马小娅, 冯超, 黄晨茜, 张博, 周金陈, 韦科龙, 郑威, 郑海英. α-亚麻酸对水牛卵巢颗粒细胞体外培养的影响[J]. 中国畜牧兽医, 2023, 50(6): 2380-2387.
[3]	王泽平, 王飞飞, 张芮铭, 付言峰, 赵为民, 戴超辉, 程金花, 廖超, 李辉. 苏紫猪SLA-1基因多态性及其抗病潜能分析[J]. 中国畜牧兽医, 2023, 50(5): 1918-1927.
[4]	张蕊, 穆春宇, 汤青萍, 卜柱, 杨明军, 夏爱萍, 杨晓明. 鸽GnIH基因多态性及其与产蛋性状的关联分析[J]. 中国畜牧兽医, 2023, 50(4): 1412-1421.
[5]	李中波, 侯强红, 段德勇. 不同种群白纹伊蚊单倍型多态性、遗传分化及种系发育关系分析[J]. 中国畜牧兽医, 2023, 50(4): 1452-1460.
[6]	谭元成, 罗华伦, 张依裕, 杨酸, 王盈童, 王春源. 白羽王鸽MyoG基因多态性及其与体尺和肉质性状的关联分析[J]. 中国畜牧兽医, 2023, 50(4): 1461-1471.
[7]	李诚兰, 郭婷婷, 岳耀敬. 基因组选择及其在羊育种中的应用研究进展[J]. 中国畜牧兽医, 2023, 50(2): 616-625.
[8]	刘泽林, 杨娟, 许铭洙, 张年, 熊琪, 刘静波, 陶虎. 山羊ZBP1基因多态性及与产羔性能的关联分析[J]. 中国畜牧兽医, 2023, 50(1): 161-173.
[9]	龙安炬, 杨酸, 艾锦新, 翁吉梅, 张依裕, 蔡惠芬, 罗卫星. 黔北麻羊MC4R基因多态性及其与生长性状的关联分析[J]. 中国畜牧兽医, 2023, 50(1): 198-209.
[10]	徐昊祺, 许静漪, 胡丽蓉, 张帆, 罗汉鹏, 张海亮, 师睿, 李想, 刘林, 刘巧香, 郭刚, 王雅春. 中国荷斯坦牛PIK3CB基因多态性及其与繁殖和产奶性状的关联分析[J]. 中国畜牧兽医, 2022, 49(9): 3438-3452.
[11]	唐庆凤, 李玲, 黄加祥, 方文远, 农皓如, 唐艳, 陆呈委, 韦盘秋, 冯玲. 广西地区不同饲养模式及不同季节水牛奶霉菌毒素污染状况调查[J]. 中国畜牧兽医, 2022, 49(9): 3665-3675.
[12]	丁梦琴, 黑达西·巴哈提, 彭凤强, 胡雅丽, 沙尔山别克·阿不地力大. 拜城油鸡FSHR基因多态性及其与生产性能的关联分析[J]. 中国畜牧兽医, 2022, 49(8): 3044-3053.
[13]	周迪, 敖叶, 蒋桂荣, 赵忠海, 李俊, 杨蓉, 龚菲, 陈昌雪, 沈银, 李辉. 黔东南小香鸡MEF2C基因外显子多态性及其与体重、体尺性状的关联分析[J]. 中国畜牧兽医, 2022, 49(7): 2613-2621.
[14]	马彦茹, 于永生, 曹阳, 秦立红, 赵玉民, 吴健. 中国草原红牛PDK4基因多态性及其与肉质性状的关联分析[J]. 中国畜牧兽医, 2022, 49(6): 2186-2194.
[15]	柴志欣, 武志娟, 王吉坤, 张成福, 钟金城, 信金伟. CTGF、Gprin3基因多态性及其与牦牛体尺性状的关联分析[J]. 中国畜牧兽医, 2022, 49(6): 2216-2227.