香猪SNP位点rs80894395的多态性及其与体尺指标之间的关联研究

doi:10.16431/j.cnki.1671-7236.2016.05.026

›› 2016, Vol. 43 ›› Issue (5): 1294-1301.doi: 10.16431/j.cnki.1671-7236.2016.05.026

香猪SNP位点rs80894395的多态性及其与体尺指标之间的关联研究

王洋^1,2, 梅子凌^1,2, 喻昌燕^1,2, 牛熙², 谢玲玲³, 牛秋锦¹, 仇志浪¹, 王嘉福^1,2, 冉雪琴³

1. 贵州大学生命科学学院, 贵阳 550025;
2. 贵州大学农业生物工程研究院, 贵阳 550025;
3. 贵州大学动物科学学院, 贵阳 550025

收稿日期:2015-12-01 出版日期:2016-05-20 发布日期:2016-05-23
通讯作者: 王嘉福 E-mail:jfwang@gzu.edu.cn
作者简介:王洋(1991-),男,安徽宣城人,硕士生,研究方向:动物生物化学与分子生物学,E-mail:596868170@qq.com;梅子凌(1990-),女,安徽芜湖人,硕士生,研究方向:动物生物化学与分子生物学,E-mail:meizilingat126@126.com
基金资助:
国家高技术研究发展计划(863计划)课题(2013AA102503);贵州省农业攻关项目(黔科合NY[2013]3073号);贵州省科技创新人才团队建设专项(黔科合人才团队2009-4006);贵州大学"本科教学工程"项目(BKJX2012007);贵州大学研究生创新基金"乙酰氨基半乳糖转移酶基因多态性研究"(研农2015018)

Polymorphism of SNP Site rs80894395 and its Association with the Body Size of Xiang Pig

WANG Yang^1,2, MEI Zi-ling^1,2, YU Chang-yan^1,2, NIU Xi², XIE Ling-ling³, NIU Qiu-jin¹, QIU Zhi-lang¹, WANG Jia-fu^1,2, RAN Xue-qin³

1. College of Life Sciences, Guizhou University, Guiyang 550025, China;
2. Institute of Agro-bioengineering, Guizhou University, Guiyang 550025, China;
3. College of Animal Science, Guizhou University, Guiyang 550025, China

Received:2015-12-01 Online:2016-05-20 Published:2016-05-23

摘要/Abstract

摘要： 为了探究影响香猪生长发育的功能基因,本试验利用Illumina Porcine SNP60K芯片筛选出N-乙酰氨基半乳糖转移酶2(polypeptide N-acetylgalactosaminyltransferase 2,GALNT2)基因内含子10中一个与腹围呈弱相关的单核苷酸多态性(single nucleotide polymorphism,SNP)位点rs80894395。在此基础上应用等位基因特异性PCR(allele-specific PCR,AS-PCR)技术检测520头雌性香猪群体中该位点的基因型,并与43头大白猪、36头柯乐猪相比较。结果表明,从3个猪群体中检测到rs80894395位点的CC、CT和TT3种基因型;其中香猪群体以CT为优势基因型,且基因型与香猪的体重、体长和腹围呈弱的正相关;大白猪群体以CC基因型为主,T等位基因频率较香猪极显著减少(P<0.01);柯乐猪群体中CC与CT基因型频率相等,与香猪和大白猪的基因频率间的差异不显著(P>0.05)。生物信息学分析表明,rs80894395位点可能影响GALNT2基因的剪接过程。rs80894395与香猪的生长发育可能有一定的联系。

关键词: 香猪; 体尺; 内含子剪接; SNP; GALNT2基因

Abstract: To explore the functional genes affecting the growth and development of Xiang pig, a single nucleotide polymorphism (SNP) site rs80894395, located at intron 10 of polypeptide N-acetylgalactosaminyltransferase 2 (GALNT2) gene was screened out by Illumina Porcine SNP60K chip, which showed a weak association with the abdomen circumference of pig.Further, the genotypes of site rs80894395 in 520 female Xiang pigs were detected using allele-specific PCR(AS-PCR) method, taking 43 Yorkshire pigs and 36 Kele pigs as control groups.All of CC, CT and TT genotypes were detected from the three pig breeds.The dominant genotype of Xiang pig was CT genotype and it was positively weak-associated with its body measurement indexes including body weight, body length, and abdomen circumference.The CC genotype was dominant genotype in Yorkshire pig, and the frequency of T allele was extremely significant decreased compared with that of Xiang pig (P<0.01).There was no difference in the frequencies of CC and CT genotypes in Kele pig, and its allele frequencies was not significantly different compared with that of Xiang or Yorkshire pig breeds (P>0.05).A splice-site nearby site rs80894395 was found out using bioinformatics prediction software, which might affect the splicing process of GALNT2 gene.It suggested that site rs80894395 might have a connection with the growth and development of Xiang pig.

Key words: Xiang pig; body sizes; intron splicing; SNP; GALNT2 gene

中图分类号:

S828

王洋, 梅子凌, 喻昌燕, 牛熙, 谢玲玲, 牛秋锦, 仇志浪, 王嘉福, 冉雪琴. 香猪SNP位点rs80894395的多态性及其与体尺指标之间的关联研究[J]. , 2016, 43(5): 1294-1301.

WANG Yang, MEI Zi-ling, YU Chang-yan, NIU Xi, XIE Ling-ling, NIU Qiu-jin, QIU Zhi-lang, WANG Jia-fu, RAN Xue-qin. Polymorphism of SNP Site rs80894395 and its Association with the Body Size of Xiang Pig[J]. , 2016, 43(5): 1294-1301.

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香猪SNP位点rs80894395的多态性及其与体尺指标之间的关联研究

Polymorphism of SNP Site rs80894395 and its Association with the Body Size of Xiang Pig

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