大薯部分替代玉米对文昌鸡盲肠微生物区系和短链脂肪酸含量的影响

doi:10.16431/j.cnki.1671-7236.2015.11.023

›› 2015, Vol. 42 ›› Issue (11): 2977-2983.doi: 10.16431/j.cnki.1671-7236.2015.11.023

大薯部分替代玉米对文昌鸡盲肠微生物区系和短链脂肪酸含量的影响

陈涛¹, 黄东益¹, 杨雨辉¹, 王学梅^1,2

1. 海南大学农学院热带动物繁育与营养实验室, 海口 570228;
2. 热带生物资源教育部重点实验室, 海口 570228

收稿日期:2015-05-07 出版日期:2015-11-20 发布日期:2015-11-26
通讯作者: 王学梅 E-mail:xmwangsnow@sina.com
作者简介:陈涛(1992-),男,湖南岳阳人,硕士生,研究方向:功能性物质的研究与应用,E-mail:tedc@foxmail.com
基金资助:
海南省重点科技项目:新型饲料大薯的开发利用(ZDXM2012002);海口市重点科技项目:新型饲料资源薯蓣的开发与综合利用(2011-0119)

Effect of Partial Replacement of Corn by Dioscorea alata L.on Microflora and Short-chain Fatty Acid Content in Cecum of Wenchang Chicken

CHEN Tao¹, HUANG Dong-yi¹, YANG Yu-hui¹, WANG Xue-mei^1,2

1. The Tropical Animal Breeding, Reproduction and Nutrition Laboratory, College of Agricultural, Hainan University, Haikou 570228, China;
2. Key Laboratory of Tropical Biological Resources of Ministry of Education, Haikou 570228, China

Received:2015-05-07 Online:2015-11-20 Published:2015-11-26

摘要/Abstract

摘要： 本试验旨在研究大薯部分替代玉米对文昌鸡盲肠微生物区系和短链脂肪酸含量的影响,探讨大薯替代玉米作为文昌鸡饲料开发的可行性。选择288只健康、体重相近的50日龄文昌鸡母鸡,随机分成4组,每组4个重复,每个重复18只鸡。对照组饲喂玉米-豆粕型基础日粮;Ⅰ、Ⅱ和Ⅲ组为试验组,分别饲喂以大薯替代基础日粮中10%、15%、20%玉米的等能等氮试验日粮。试验期28 d。结果表明,经过14 d饲养试验,大薯替代不同比例玉米对文昌鸡盲肠微生物区系无显著影响(P>0.05),对文昌鸡盲肠内容物短链脂肪酸含量影响显著(P<0.05),其中Ⅲ组丙酸、丁酸含量显著低于对照组(P<0.05)。随着饲养期延长至28 d,大薯替代不同比例玉米对盲肠微生物区系和短链脂肪酸含量影响显著(P<0.05),其中Ⅱ组大肠杆菌数量显著低于对照组(P<0.05),Ⅲ组粪链球菌、总厌氧菌数量分别显著和极显著高于对照组(P<0.05;P<0.01);Ⅰ组丁酸和丙酸含量分别显著和极显著低于对照组(P<0.05;P<0.01)。综上所述,大薯部分替代玉米能提升盲肠有益菌群数量,但短期饲喂会降低短链脂肪酸生成,长期饲喂对短链脂肪酸生成无显著影响。在本试验条件下,建议大薯替代玉米比例以15%为宜。

关键词: 大薯; 玉米; 文昌鸡; 微生物; 短链脂肪酸

Abstract: This study was conducted to investigate the effect of partial replacement of corn by Dioscorea alata L.on microflora and short-chain fatty acid content in cecum of Wenchang chicken.A total of 288 healthy Wenchang chickens at 50 days of age with the similar body weight were divided into 4 groups with 4 replicates per group and 18 chickens per replicate.Chickens in the control group were fed a basal diet (corn-soybean diet),and the others in the experimental groups (group Ⅰ,Ⅱ and Ⅲ) were fed three isonitrogenous and isoenergetic experimental diets which using Dioscorea alata L.replaced 10%,15%,20% corn,respectively.The trial lasted for 28 days.The results showed that after 14 days of feeding trials,replacement of different proportion of corn by Dioscorea alata L.could not significantly affect the microflora in cecum (P>0.05),but could significantly affect the short-chain fatty acid content (P<0.05),the contents of propanoic acid and butyric acid in group Ⅲ were significantly lower than that in control group (P<0.05).After 28 days of feeding trials,replacement of different proportion of corn by Dioscorea alata L. could significantly affect the microflora and the short-chain fatty acid content in cecum (P<0.05),the number of Escherichia coli in group Ⅱ was significantly lower than that in control group (P<0.05),and the number of Streptococcus faecalis and total anaerobic bacteria in group Ⅲ were significantly and extremely significantly higher than that in control group (P<0.05;P<0.01),respectively;The content of butyric acid and propanoic acid in group Ⅰ were significantly and extremely significantly lower than that in control group (P<0.05;P<0.01),respectively.In conclusion,partial replacement of corn by Dioscorea alata L.could promote the number of beneficial bacteria in cecum,but suppress the short-chain fatty acid content in cecum of Wenchang chicken in a relatively short time,however,no significant effect showed in the short-chain fatty acid content after 28 days of feeding.The suitable replacement proportion was 15% under this experiment conditions.

Key words: Dioscorea alata L.; corn; Wenchang chicken; microflora; short chain fatty acid

中图分类号:

S831

陈涛, 黄东益, 杨雨辉, 王学梅. 大薯部分替代玉米对文昌鸡盲肠微生物区系和短链脂肪酸含量的影响[J]. , 2015, 42(11): 2977-2983.

CHEN Tao, HUANG Dong-yi, YANG Yu-hui, WANG Xue-mei. Effect of Partial Replacement of Corn by Dioscorea alata L.on Microflora and Short-chain Fatty Acid Content in Cecum of Wenchang Chicken[J]. , 2015, 42(11): 2977-2983.

参考文献

[1] Odimegwu J I,Odukoya O,Yadav R K,et al.A new source of elemol rich essential oil and existence of multicellular oil glands in leaves of the Dioscorea species[J].The Scientific World Journal,2013,2013:1-6.
[2] 王学梅,李笑春,黄东益,等.饲料中添加大薯对文昌鸡生长性能、屠宰性能和肉质品质的影响[J].中国饲料,2014,4:23-26.
[3] 王学梅,许丽,黄东益,等.大薯在鸡日粮中能量及常规养分营养价值评定[J].中国饲料,2013,23:5-8.
[4] 黄洁,甘学德,许瑞丽,等.21份紫肉甘薯种质资源的营养价值及其产量评估[J].福建农业学报,2011,26(2):215-222.
[5] McNeeley D F,Brown A E,Noel G J,et al.An investigation of vancomycin-resistant Enterococcus faecium within the pediatric service of a large urban medical center[J].The Pediatric Infectious Disease Journal,1998,17(3):184-188.
[6] Lan Y,Williams B A,Tamminga S,et al.In vitro fermentation kinetics of some non-digestible carbohydrates by the caecal microbial community of broilers[J].Animal Feed Sci Techno,2005,123:687-702.
[7] Corrier D E,Nisbet D J,Hollister A G,et al.Development of defined cultures of indignous cecal bacteria to control salmonellosis in broiler chickens[J].Poultry Science,1993,72(6):1164-1168.
[8] Rastall R A,Gibson G R,Gill H S,et al.Modulation of the microbial ecology of the human colon by probiotics,prebiotics and synbiotics to enhance human health:An overview of enabling science and potential applications[J].FEMS Microbiol Ecol,2005,52(2):145-152.
[9] Malik R K,Montecalvo M A,Reale M R,et al.Epidemiology and control of vancomycin-resistant enterococci in a regional neonatal intensive care unit[J].Pediatr Infect Dis,1999,18(4):352-356.
[10] 王金全.小麦非淀粉多糖的抗营养机理及木聚糖酶在肉仔鸡小麦日粮中的应用[D].北京:中国农业科学院,2004.
[11] Marounek M,Suchorska O,Savka O.Effect of substrate and feed antibiotics on in vitro production of volatile fatty acids and methane in caecal contents of chickens[J].Animal Feed Science and Technology,1999,80(3):223-230.
[12] Marounek M,Dušková D,Skrivanova V.Effect of non-ionophore feed antibiotics on in vitro fermentation in the ovine rumen and rabbit caecum[J].The Journal of Agricultural Science,1998,130(1):115-118.
[13] 蒋小丰,方热军.丁酸在动物体内的作用[J].饲料工业,2008,29(20):51-54.
[14] Campbell G L,Campbell L D,Classen H L.Utilisation of rye by chickens:Effect of microbial status,diet gamma irradiation and sodium taurocholate supplementation[J].British Poultry Science,1983,24(2):191-203.
[15] 秦雪梅,刘静,霍贵成.低聚糖与水溶性膳食纤维对婴儿肠道菌群益生作用研究[J].食品科技,2011,36(6):96-100.
[16] 徐晓燕,卜登攀,孙鹏,等.日粮添加不同比例的中短链和长链脂肪酸对奶牛免疫功能的影响[J].中国畜牧兽医,2012,39(10):108-112.
[17] 哈斯额尔敦,胡菡,王加启,等.2008-2009年反刍动物营养研究进展[J].中国畜牧兽医,2010,37(3):11-18.
[18] 罗剑鸣,饶洁,冯嘉韵,等.3种不溶性复合膳食纤维对小鼠肠道内短链脂肪酸产生的影响[J].食品科学,2012,33(3):225-227.

大薯部分替代玉米对文昌鸡盲肠微生物区系和短链脂肪酸含量的影响

Effect of Partial Replacement of Corn by Dioscorea alata L.on Microflora and Short-chain Fatty Acid Content in Cecum of Wenchang Chicken

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