苜蓿黄酮对杂交绵羊瘤胃发酵功能和纤维降解酶活性的影响

doi:10.16431/j.cnki.1671-7236.2015.11.022

›› 2015, Vol. 42 ›› Issue (11): 2969-2976.doi: 10.16431/j.cnki.1671-7236.2015.11.022

苜蓿黄酮对杂交绵羊瘤胃发酵功能和纤维降解酶活性的影响

王梦竹^1,2, 刘艳丰^1,2, 王文奇², 侯广田², 张文举¹

1. 石河子大学动物科技学院, 石河子 832000;
2. 新疆畜牧科学院饲料研究所, 乌鲁木齐 830000

收稿日期:2015-06-03 出版日期:2015-11-20 发布日期:2015-11-26
通讯作者: 刘艳丰 E-mail:liuyanfeng520@sina.com
作者简介:王梦竹(1988-),女,山东聊城人,硕士生,研究方向:动物营养与饲料,E-mail:349057724@qq.com
基金资助:
新疆维吾尔自治区公益性科研院所基本科研业务经费资助项目(KY2014006);自治区科研院所改革与发展专项资金(2015015);国家现代肉羊产业技术体系(nycytx-39)

Effect of Alfalfa Flavonoids on Rumen Fermentation Function and Cellulolytic Enzymatic Activity of Crossbreed Sheep

WANG Meng-zhu^1,2, LIU Yan-feng^1,2, WANG Wen-qi², HOU Guang-tian², ZHANG Wen-ju¹

1. College of Animal Science and Technology, Shihezi University, Shihezi 832000, China;
2. Feed Research Institute, Xinjiang Academy of Animal Science, Urumqi 830000, China

Received:2015-06-03 Online:2015-11-20 Published:2015-11-26

摘要/Abstract

摘要： 本试验旨在研究苜蓿黄酮对杂交绵羊瘤胃发酵功能和纤维降解酶活性的影响。选择体重为27.02 kg±3.03 kg、安装有永久性瘤胃瘘管的萨福克羊×小尾寒羊杂交F1代绵羊(公羊)12只,采用单因素随机区组试验设计,分成4组,每组3个重复,每个重复1只。对照组饲喂基础日粮,试验组分别在基础日粮中添加0.1%、0.2%和0.4%苜蓿黄酮。预试期7 d,正试期41 d。结果表明:①与对照组相比,添加苜蓿黄酮12 h后,0.2%组pH显著升高(P<0.05),其余各组差异均不显著(P>0.05);各试验组氨态氮(NH₃-H)浓度均无显著变化(P>0.05),浓度变化幅度随剂量增加而变大。②与对照组相比,0.1%和0.4%组乙酸、丙酸、丁酸、戊酸和总挥发性脂肪酸的平均浓度均提高,0.2%组则相反;添加苜蓿黄酮2 h后,0.1%、0.2%组异戊酸浓度显著高于0.4%组(P<0.05)。③与对照组相比,0.1%和0.2%组木糖酶、内切葡聚糖酶、纤维二糖酶和纤维素酶活性均下降,0.4%组则升高,但差异均不显著(P>0.05)。综上所述,日粮中添加苜蓿黄酮可维持杂交绵羊瘤胃发酵功能稳定,不同剂量苜蓿黄酮对纤维降解酶活性作用不同。在本试验条件下,建议苜蓿黄酮在杂交绵羊日粮中的添加量为0.4%。

关键词: 苜蓿黄酮; 瘤胃发酵; 纤维素降解酶; 活性

Abstract: This study was performed to evaluate the effects of alfalfa flavonoids on rumen fermentation function and cellulolytic enzymatic activity of crossbreed sheep.A single factor random block design was used,and twelve crossbreed sheep (F1) Suffolk×Small-tail Han sheep with permanent rumen fistulas and the body weight of 27.02 kg±3.03 kg were selected and randomly divided into 4 groups with 3 replicates per group and 1 single sheep per replicate.The control group was fed a basal diet without alfalfa flavonoids,and the experimental groups were fed the basal diets supplemented with 0.1%,0.2% and 0.4% alfalfa flavonoids,respectively.The pre-test period lasted for 7 days and the trial period lasted for 41 days.The results showed as follows:①Compared with control group,the pH of 0.2% group was significantly increased at 12 h after adding alfalfa flavonoids (P<0.05),and no significant differences were observed in other groups (P>0.05);There were no significant differences in NH₃-H concentration among all groups (P>0.05),and the concentration was increasing with the dose rising (P>0.05).②Compared with control group,the average concentrations of acetate,propionate,butyrate,valerate and TVFA in 0.1% and 0.4% groups were increased,whereas the concentrations in 0.2% group showed contrary pattern.The isovalerate concentration in 0.1% and 0.2% groups were significantly higher than that in 0.4% group at 2 h after adding alfalfa flavonoids (P<0.05).③Compared with control group,the activities of xylanase,endoglucanase,cellobiase and cellulase in 0.1% and 0.2% groups were decreased,but those in 0.4% group were increased and the difference was not significant (P>0.05).In conclusion,dietary supplementation of alfalfa flavonoids could keep the stability of rumen fermentation function of crossbreed sheep and this compound in different dose had different effect on cellulolytic enzymatic activity.The recommended supplemental level of alfalfa flavonoids was 0.4% in diet for crossbreed sheep under this test condition.

Key words: alfalfa flavonoids; fermentation function; cellulolytic enzymatic; activity

中图分类号:

S826

王梦竹, 刘艳丰, 王文奇, 侯广田, 张文举. 苜蓿黄酮对杂交绵羊瘤胃发酵功能和纤维降解酶活性的影响[J]. , 2015, 42(11): 2969-2976.

WANG Meng-zhu, LIU Yan-feng, WANG Wen-qi, HOU Guang-tian, ZHANG Wen-ju. Effect of Alfalfa Flavonoids on Rumen Fermentation Function and Cellulolytic Enzymatic Activity of Crossbreed Sheep[J]. , 2015, 42(11): 2969-2976.

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苜蓿黄酮对杂交绵羊瘤胃发酵功能和纤维降解酶活性的影响

Effect of Alfalfa Flavonoids on Rumen Fermentation Function and Cellulolytic Enzymatic Activity of Crossbreed Sheep

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