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Table of Content

20 November 2007, Volume 34 Issue 11
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动物营养与饲料科学
Effect of Copper Complexes Derived from Schiff ’s Base of Lysine on the Rumen Fermentation of Sheep
HU Jianyong;YANG Kailun;NIU Chaofeng;SU Lingling;ZHU Wentao
2007, 34(11):  5-8. 
Abstract ( 1379 )  
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Four male Xinjiang Merino sheep,with 40 kg of average live weight,fitted with permanent cannulas in the rumen,were supplemented with three dosages (0,4,8 g/sheep·d)of Copper complexes derived from Schiff’s base of Lysine in three periods respectively to study the effect of Copper complexes derived from Schiff’s base of Lysine on rumen fermentation. The results showed that the intake of DM of corn straw was increased 12.5％at the level of 8 g/sheep·d（P＜0.05）. The dosage of 4 g/sheep·d had no effect on rumen pH value（P＞0.05）,but that of 8 g/sheep·d tended to slow up the decrease of rumen pH value 3 h after feeding. The dosage of 4 g/sheep·d did not influence the concentration of ammonia nitrogen（P＞0.05）,but that of 8 g/sheep·d decreased the concentration of ruminal ammonia nitrogen. The ruminal concentrations of acetic and total volatile fatty acid (TVFA) were decreased,however,the propionic acid and butyric acid were increased,and rumen fermentation type was changed from acetic to propionic acid after addition of the Copper complexes derived from Schiff’s base of Lysine. This experiment indicated that the Copper complexes derived from Schiff base of Lysine could regulate the rumen fermentation. 
Effects of Alfalfa Glycosides on Anti-oxidation Performance in Broilers
ZHANG Yong;WANG Jing;LIN Dongkang
2007, 34(11):  11-15. 
Abstract ( 1501 )  
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A total of 96 1day healthy AA broilers of commercial strain were chosen in this experiment, the birds were divided into 3 groups: control, test groupⅠand Ⅱ, four replicates of 8 birds were in a group. The total experimental time was 49 days. During 1～21 days, birds in test groupⅠand Ⅱ were fed basal diet supplemented with 0.1% alfalfa glycosides, respectively. During 22～35 days and the 36～49 days, birds in groupⅠwere fed basal diet supplemented with 0.1% alfalfa glycosides, and birds in groupⅡ were fed basal diet supplemented with 0.2 % alfalfa glycosides. The results showed that: ①Dietary supplementation of 0.1% alfalfa glycosides effects significantly the feed intake(FI) of the broilers in 22～35 days，but the performance difference of other groups and experimental times is not significant (P>0.05). ②Dietary supplementation of alfalfa glycosides to the different date age has the influence on serum and liver GSHPx, SOD and MDA level in different extend.
Studies of Saccharicterpenin on the Immunity Function of Piglets
TANG Xiaoling;LIU Xiaofei;WEN Guihui;QIU Weihai;ZHANG Shirui
2007, 34(11):  16-19. 
Abstract ( 1382 )  
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A study was conducted to evaluate the Saccharicterpenin effects on production performance, intestinal canal micro-flora population, biochemical parmeter of blood on the piglets early weaning(PEW). Treatments were as follows: treatment Ⅰ is the control group, the group treatment Ⅱ,Ⅲ,Ⅳ,Ⅴ respectly contained 250 mg/kg Saccharicterpenin, 500 mg/kg Saccharicterpenin, 250 mg/kg Saccharicterpenin plus 4 mg/kg Flavomycin, 4 mg/kg Flavomycin. The results showed that: Supplemented with 500 mg/kg Saccharicterpenin of Flavomycin plus 250 mg/kg Saccharicterpenin, the number of TP、GLO、G/A were improved, the group treatment Ⅲ is the best; IgA is significant improved(P<0.05), the group treatment Ⅱ,Ⅲ,Ⅳ,Ⅴ was higher 43.21%, 104.89%,73.21%,27.74% than the control, IgG,the group treatment Ⅱ,Ⅲ,Ⅳ,Ⅴ was higher 10.50%,12.07%,11.05%,10.26% than the control, group; treatment Ⅱ,Ⅲ,Ⅳ,Ⅴ on the Serum of BUN was higher 2.67%,2.96%, 2.81%, 2.52% than the control, but no significant difference to GLU(P>0.05), The number of AST, ALT, ALP of the group treatment Ⅲ was higher than others, but the group treatment Ⅱ,Ⅲ,Ⅳ,Ⅴ of LDH was lower 2.71%,4.02%, 2.96%, 1.65% than the control.
The Effect of Vitamin E Deficiency on Antioxidation and Fluidity of Lymphocyte Membrane in Chicken
WANG Ben;LI Yanfei;FENG Jiawang;ZHANG Jinlong
2007, 34(11):  20-22. 
Abstract ( 1862 )  
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The activity of SOD and GSHPx and the level of MDA of lymphocyte membrane in the modle chicken with Vitamin E (VE)deficiency that produced by using low content of VE feedstuff and supplying unsaturated fatty acid in the diet were detected by Test Kit;the membrane fluidity of lymphocyte in the same modle chicken was detected through fluorescence polarization. The results showed that activity of SOD and GSH-Px and fluidity of lymphocyte membrane were significantly lower in experimental chicken than control ones(P<0.05，P<0.01);the MDA content were significantly higher than control ones (P<0.05).The results indicated that VE plays an important role in maintaining the function of lymphocyte membrane in chicken. 
Effect of Fat on Immune Function of Poultry
WANG Xinwei;JIA Qiang;ZHOU Yan
2007, 34(11):  22-25. 
Abstract ( 1301 )  
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Nutrition factors have an important role in immunity of domestic birds. The fat is one of necessary nutrition elements for them. The fat is indispensable component ingredients and nutriment of poultry, which not only keeps poultry’s growing, breeding, and producing normally, but also has a great effect on poultry’s immune organ, tissue and regulate its immune response. So, a survey was made on the effect of fat on poultry’s immune function such as immune organ growth, body’s immunity, cell-mediated immunity, non-special immunity, and production of Cytokines. This could provide the theory basis for preparing rational diets of poultry and improving their ability against diseases in poultry’s production practice.
遗传繁育
Analyzing of Genetic Diversity of Pinghu Royal Jelly Bee and Suwang NO.1 Bee with Microsatellite Markers
JI Ting;CHEN Jing;MENG Chunling;BAO Wenbin;CHEN Guohong
2007, 34(11):  32-35. 
Abstract ( 1293 )  
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Genetic diversity of Pinghu royal jelly bee and Suwang No.1 bee were evaluated with 6 microstaellite loci, the genetic variability within breeds and genetic differentiation between breeds were estimated, the results showed that in 6 microstaellite loci, the 40 alleles were found, the number of alleles per locus ranged from 4 to 10, the average expected heterozygosity and PIC of all loci were 0.5078 and 0.4708 respectively. Mean numbers of effective alleles of Pinghu royal jelly bee and Suwang No.1 bee were 0.4981 and 0.5007, inbreeding index(FST), Reynolds' genetic distance and gene flow between two populations were 0.037,0.03770 and 6.507 respectively, these results indicated that the heterozygosity and the genetic diversity of two bee populations were very high.
PCR-SSCP Analysis of Prolactin Gene in Goats
ZHAO Xiuhua;CHU Mingxing;WANG Jinyu;FANG Li;YE Sucheng
2007, 34(11):  35-38. 
Abstract ( 1386 )  
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The prolactin (PRL) gene was studied as a candidate gene for the prolificacy of Jining Grey goats. Single nucleotide polymorphisms of exon 1 to exon 5 of PRL gene were detected in high fecundity breeds (Jining Grey, Boer, Wendeng dairy goat) and low fecundity breed (Inner Mongolia Cashmere goat) with five pairs of primers by PCR-SSCP. For the first time in goats, the entire exon sequence of PRL gene was obtained by means of clone sequencing. No single nucleotide polymorphism was detected in four goat breeds. This result indicated that the detected loci of PRL gene had no significant effect on prolificacy of Jining Grey goats.
Effects on Embryonic Stem Cells and Influencing Factors of Isolation & Culture of Feeder Cells
WANG Ying;CHEN Lina;ZHANG Yanyan;GUAN Weijun
2007, 34(11):  39-42. 
Abstract ( 1456 )  
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Comprehensive research of stem cells which is one of the energiest,most impacting and applied foreground research directions in life science,when united with genetic engineering and transplantation may provide significance of theory and practice.Feeder cells have a great impact on isolation and culture of ES cells.Refering to plenty of information,the author combined with experiment experience explained the general impact of feeder cells on embryonic stem cells, introduced the class of feeder cells and discussed the influencing factores of isolation and culture.
GATA Family in Mammalian Reproduction
HE Yuanqing;CHU Mingxing;LIU Rongzhi;WANG Jinyu
2007, 34(11):  42-45. 
Abstract ( 1333 )  
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In this paper we briefly introduced the sorts, structure, location of GATA transcription factor family, and discussed the regulation action of GATA factors in mammalian reproductive development and function in detail.
Detection of Goat Callipyge Genotype
WANG Hailiang;LI Xianglong;ZHOU Rongyan;LI Lanhui;GUO Xiuli
2007, 34(11):  49-51. 
Abstract ( 1373 )  
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The 493bp fragment of Callipyge gene of 113 individuals was amplified by PCR, of which 35 were from Boer goat, 38 were from Nanjiang brown goat and 40 were from Chengde polled goat. There was a variation of C184T discovered by sequencing. Molecular genetic diversity of Callipyge gene with restriction endomuclease Fok I was studied by PCR-RFLP. The polymorphism was existed in Boer goat and Nanjiang brown goat. There were 3 genotypes, which was CC, CT and TT, respectively. The frequency of genotypes was 0.6571, 0.3143 and 0.0286 respectively in Boer goat. The frequency of genotypes was 0.8947, 0.1053 and 0.0000 respectively in Nanjiang brown goat. However, there is all CC genotype in Nanjiang brown goat. It suggested that there was a relationship of the variation of C184T to the trait of Callipyge in goat. 
生物技术
H5N1 Subtype AIV Strains Plaque Clone and the Biological Characteristics Comparison
YANG Su;;HUANG Qingyun;LIAO Ming;REN Tao;SHA Caihua;LIAO Xiuyun
2007, 34(11):  54-60. 
Abstract ( 1842 )  
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With the method developed by our research, using agar without neutral red, plaques were picked under an inverted microscope, we plaque-purified 3 strains H5N1 subtype AIV chicken embryo isolates and obtained thirteen plaque-purified strains with marked difference in size and shape. Our research indicate addition of suitable amount of trypsin could facilitate plaque formation with marked increase in plaque diameter and plaque-forming units, its optimal concentration being 50 μg/ml, when the concentration of calf serum is 2%. TCID50, EID50 and LD50 testing were done on embryo isolates and the plaque-purified strains, when It was found in the study that for the size and shape of purified strains which came from the same embryo isolate exist notable difference, the larger the plaque the higher the virulence, and that the virulence of the largeplaque strains approached that of the embryo isolates, which indicate that virus particles producing large plaques were dominant in embryo isolates. Analysis of the nucleotide sequences and deduced amino acid sequences of HA genes of the 13 plaque-purified strains revealed that the cleavage site amino acid sequences of the HA protein were all conforming to structures assigned to the molecular characteristics of highly pathogenic notifiable avian influenza virus.
The Molecular Sexing of Balearica Pavonina
HU Fei;ZHANG Hongxia;BAO Wenbin;WU Yunliang;CHEN Guohong
2007, 34(11):  61-63. 
Abstract ( 1896 )  
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Balearica pavonina has a small quantity and it's significant to develop artificial reproduction and breeding. This research extracted genome DNA from feathers and amplified the EE0.6 gene of Balearica pavonina specifically with two combinations of primers. The results showed that male birds displayed one distinct amplification band，whereas two bands were amplified for female ones. With the results, the sex of Balearica pavonina could be identified successfully. It is meaningful for the reproduction of the birds.
Construct the PK-15 Cells that Express E0 Protein of HCLV
KANG Wenhua;HAO Junfeng;PAN Chungang;NING Yibao
2007, 34(11):  63-65. 
Abstract ( 1669 )  
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E0 gene was taked back from the pGEM-T Easy-E0 recombinant plasmid cleaved by BamH I and Pst I, was cloned into the EGFP. High pure recombinant plasmid of pEGFP- E0 was transfected into PK-15 cell, BHK-21 cell, VERO cell by LipofectamineTM2000, examine the cell for fluorescence using a UV microscope. All could find the fluorescence in PK-15 cells, BHK-21 cells, VERO cells. In 24 h, 48 h and 72 h, count of the the cell for fluorescence were increasing, count of the cell for fluorescence were most in PK-15 cells, BHK-21 cells in 72 hs, VERO cells. count of the cell for fluorescence were most in PK-15 cells in 72 h. Detection indicated expressed E0 protein mainly locate in the plasma of the PK-15 cells. After cleaved by BamH I and Pst I, PCR and indirect immuno fluorescence assay, detection indicated large number of expressed E0 protein are in the PK-15 cells.After PK-15 cells and PK-E0 cells infected by HCLV, the titer of HCLV was the highest in 72 h, then began to decrease. Detection indicated large number of expressed E0 protein caused increase of the titer of HCLV.
生理生化
The Anatomic Study on the Heart of the Wuzhishan Miniature Pig Inbred-line
JIN Erhui;PENG Kemei;ZHANG Yong;SHAN Tongling;MU Yunian;FENG Shutang;LI Kui;LIU Huazheng;TANG Wenhua;YANG Shulin
2007, 34(11):  66-70. 
Abstract ( 2241 )  
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To observe and measure the Cardiac anatomic characteristic of Wuzhishan miniature pig inbredline, twenty individuals with different month-age were anatomized, and obtained the related anatomic parameters. This study showed that the cardiac gross morphology, the chambers division of heart and cardiac internal structure in the 2 and 4 months Wuzhishan miniature pig were the same, and in accord with that of human, but the cardiac size and some anatomic parameters were markedly different from that of adult, the diameter of blood vessel in the cardiac basal part were different from that of other local pig breeds and human. The anatomic study on the heart provided the basic data for the utilization of the Wuzhishan miniature pig inbredline for comparative medicine and clinical xenotransplantation.
Effect of Nanometer Titanium Dioxide on the Activity of Cellulase
CHEN Qinyu;SHI Jun;PAN Yong;WU Shimin
2007, 34(11):  70-72. 
Abstract ( 1353 )  
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In order to study the reaction and mechanism of nanometer titanium dioxide with macromolecule. The effects of different concentrations of nanometer titanium dioxide treated with different reaction time, temperature, visible light and UVA on the cellulase activity were reported in this paper. The results indicated that TiO2 treated with visible light and UVA could make cellulase inactive. And the inactive effect is in direct proportion to the concentration, temperature and the reaction time.
奶业专栏
The Effect of Supplementation of Rapeseed，Rape Oil，Fatty Acid on Milk Yield and Quality of Cows
CHEN Shengchang
2007, 34(11):  73-76. 
Abstract ( 1660 )  
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36 healthy dairy cows whose milking days are 90-150,hypo-padding are 25，LW are similar were selected，and divided into four groups （P>0.05）according to Complete Random Design. Each group received one treatment,group4 was control group, group1,2 and 3 supplemented rapeseed, rape oil and fatty acid calcium respectively。After 30days experimentation, the results shown the daily milk yield of group 1,group2 and group3 enhanced 0.88 (P<0.05), 0.31(P>0.05), 0.31(P>0.05),0.70(P<0.05) kg/h/d . No significant difference fund Between all groups' DMI and FCR; Even SNF shown an increased tendency, supplementation of rapeseed (350g/h/d), rape oil (150g g/h/d) and fatty acid (200g g/h/d) did not affect the composition of milk SNF（P>0.05）. Supplementation of fatty acid (200g g/h/d) increased milk fat significantly, but .that of rapeseed (350g/h/d), rape oil (150g g/h/d) had no any positive effect on the composition of milk SNF (P>0.05). group1 profited extract 1.08 Yuan kg/h/d, group2 lost 0.06 Yuan, group3 lost 0.14 Yuan kg/h/d;Above results shown supplemented rapeseed (350g/h/d) to the dairy cow whose milking day are 90-150, increase economical efficiency, supplementation of fatty acid enhanced the milk quality.
RAPD-PCR Typing of Staphylococcus Aureus Isolated from Bovine Mastitis
WANG Lin;YANG Xueyun;WANG Zhicai;LI Jianjun;CONG Fu
2007, 34(11):  76-78. 
Abstract ( 1539 )  
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16 Staphylococcus aureus strains isolated from bovine mastitis of two intensive dairy farms were typed with RAPDPCR. Amplification with 3 primers, only primer OLP11 produce band patterns that have characteristics. Selection of anneal temperature and concentration of template shows that 33℃ and original concentration could produce clear, distinguishable and repeatable band patterns. The result shows that 2 groups were divided for the 16 strains and 14 belong to same group. Related coefficient of the band patterns for most strains is only 0.04, which demonstrated that these strains have similar genetic background.
The Clinical Data Count of 364 Operation Cases of Dairy Cattle DislocationAbomasis and Experience on Their Diagnose and Treatment
SHI Dongmei;LI Deyin;HUANGFU Heping
2007, 34(11):  79-81. 
Abstract ( 1363 )  
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364 head of cattle were operated for Dislocation abomasis. Judging from the clinical data, we find that Left displacement of the abomasums has 295 examples, accounts for 81.04%, Right displacement of the abomasum has 69 examples, accounts for 18.96%, there are 231cases among the cows of the 1st parity, accounts for 63.46%, 84 cases among the cows of the 2nd parity, accounts for 23.08%,37 cases among the cows of the 3rd parity, account for 10.16%,9 cases among the cows of the 4th parity, account for 2.47%,2 cases of the cows above the 5th parity, account for 0.55%. 357 head of cattle were cured, the cure rate is 98.08%. Besides, other information has also been carried out counting, such as, the morbidity among different season, the serum electrolyte changes, the regular rumination and defecation of the illed cattle after the operation.
IVF Embryo of Cow Different Culture Method Contrast Experiment
DONG Zhengde;TONG Guizhi;LI Xintao
2007, 34(11):  82-84. 
Abstract ( 1306 )  
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As experiments the material take the slaughter house cow ovary, studies in vitro, in vivo cultivates the method of preserving health to the external fertilization embryonic development influence. (1) IVF embryo in vitro culture system whether adds the oviduct epithelial cell monolayer (granular cell monolayer)(2×106/ml) in nutrient fluid culture and temporary acceptor sheep in vivo raise contrast experiment. Result demonstration that, In the culture 8 d BL embryo growth rate not remarkable difference, in vitro culture system BL embryo forms mainly concentrates in 8 d(fertilization date for 0 d). In vivo culture system BL embryo’s formation most in 7 d. Adds the somatic cell in vitro culture system and in vivo cultivates the method of preserving health BL embryo production rate not remarkable difference, but remarkable is actually better than not add the somatic cell simple in vitro to cultivate the method. (2)two methods to produce the embryo the procedure to freeze, the thawing sensitivity test. Result demonstration that, After in vivo cultivates the method of preserving in vivo and the ordinary in vitro law production embryo defrosts. the survival percentage (90% VS 60%) and the embryo hatching rate (85.7% VS 44.4%)have the remarkable difference (P＜0.05）.
疾病防治
Pharmacological Action and Research Progress of Flos Lonicerae
WANG Linqing;CUI Baoan;;ZHANG Hongying;
2007, 34(11):  91-95. 
Abstract ( 1762 )  
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Flos lonicerae is a useful traditional Chinese gerbal.It can antagonize pathogenic microorganism,enhance immune function,eliminate resistance plasmid,anticatarrhals,decline fever,detoxification,protect liver and so on.Most of the effective chemical compositions of flos lonicerae conclude organic acid mostly of chlorogenic acid,flavonoid mostly of luteolin,triteolin,essential oil,etc.The authors introduce the progress of research on chemical constutents and pharmacological action of flos lonicerae.
The Analysis on Morbidity Causatio Selfbiting Disease
LI Yumei;BAI Xiujuan
2007, 34(11):  105-107. 
Abstract ( 1283 )  
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Outline the occurrence of selfbiting disease leading to the deterioration of mink skin and damage to breeding mink industry.Summarize the different pathogeny of mink selfbiting disease from scholars home and abroad。Lay emphasis on investigation overview of germ infection、microelement deficiency、heredity and other factors.Provide reference for further research on pathogeny and effective prevention of selfbiting disease.
兽医临床
The Yak Echinococcosis Investigation and Prevention System
TIAN Shengzhu;QI Zhimei
2007, 34(11):  115-117. 
Abstract ( 1793 )  
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In 1983 after the investigated discovered Banma County is yak Echinococcosis 1937 heads. The rat of the disease is 99% after some prevention action, for example feeding them Albendazoie Tablets and Praziquante Tablets, infection rate went down to 85.7% and 64%, in 1997 and in 2005, respectively.
The Tibetan Canine Parvovirus’s Diaghose and Prerention Cure
ZHANG Jifu;ZHAO Wenxin
2007, 34(11):  119-120. 
Abstract ( 1935 )  
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The 23 tibetan canine dog nhich is found in banma county is similar to canine parvovirus’s symptom,through the chnic symptom and dissection test and epidemiology’s observe combining cusist of experiment test,it was diagnvsed as canine parvovirus we use the monoclcnal antibodles immunity blood serum and ceftriaxone sodium for scientific preuention and cure and there have 11canines had been cured,the rate is 48%.
公共卫生
Study on the Multi Residues of Fluoroquinolones by Enzyme Immunoassay
PAN Xiaocheng;QI Kezong;ZHU Liangqiang;ZHAO Ruihong
2007, 34(11):  126-129. 
Abstract ( 750 )  
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The antiserum was prepared with the mixture of OFL-BSA,DIF-BSA and NORBSA.The Ci-ELISA method for the detection of ofloxacin ,difloxacin and norfloxacin was established with it.To ofloxacin ,difloxacin and norfloxacin.The regression equations about their standard curves were y=-0.1984x+0.9687(r=0.9885),y=-0.1738x+0.9150 (r=0.9985) and y=-0.2004x+0.9572 (r=0.9954),respectively.The 50% inhibition concentration(IC50) were 231.57,245.32 and 186.14ng/ml,respectively. The limits of detect (LOD) were 1.62,1.86 and 1.07ng/ml ,respectively. The coefficients of variation for the Ci-ELISA method were less than 5.44% in intra-assay and 12.45% in inter-assay. The recoveries in milk ranged from 71.70% to 94.53%.
畜禽产品
Study on Beef Quality Effect by Nutrition Level
LI Shiyou;XU Ying;LI Qihua;DUAN Gang;LIANG Yinghai;YANG Guorong
2007, 34(11):  132-134. 
Abstract ( 1757 )  
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Random selected 40 heads of 18 months-cross-breds and devided into 5 group which were 4 explemental group and 1 control group and that were fed 4 level supplements in 3 month time fedlot trail. Slaughter 5 finished cattle which were random selected from each treatment group and that for meat quility test, such as water, croud prtein, fat, ash, phosphorus, pH, tender, waterloss rate, cooked rate, the diameter of musel fibre, colour, ect.
经验交流
Study on Variations of Fiber Diameter in Different Types of Fine Wool
DI Jiang;LI Yong;LA Zati;LI Wuchen;CHEN Weijiang
2007, 34(11):  139-141. 
Abstract ( 1336 )  
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150 wool samples including 3 types of fineness(≤18 μm，18～20 μm and ≥20 μm) were taken, and the variations of fiber diameter along and within staple were studied. The results demonstrate that the variation of fiber diameter along staple is similar in different types of fineness and agrees with the seasonal change of feedstuff, and the wool with fineness being less than 18 μm has the least variations of fiber diameter（P<0.01）; Contrarily, the variation of diameter between fibers within staple is increased in company with the reducing of fineness（P<0.01）.
Study on Morphologic Parameters of Liaoning Cashmere Goat
JIANG Huaizhi;YANG Yujiang
2007, 34(11):  142-143. 
Abstract ( 1960 )  
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Through the observation method of anatomy and the histology appearance
on liaoning cashmere goat's reproductive organs ,gained anatomy parameters of main reproductive organs of liaoning cashmere goat concluding adult ram ,yong ram,adult ewe,and yong ewe. Difference were appeared between adult goat and yong goat in the parameters and the speed of growth.