山羊催乳素基因PCR-SSCP分析

Abstract

Abstract: The prolactin (PRL) gene was studied as a candidate gene for the prolificacy of Jining Grey goats. Single nucleotide polymorphisms of exon 1 to exon 5 of PRL gene were detected in high fecundity breeds (Jining Grey, Boer, Wendeng dairy goat) and low fecundity breed (Inner Mongolia Cashmere goat) with five pairs of primers by PCR-SSCP. For the first time in goats, the entire exon sequence of PRL gene was obtained by means of clone sequencing. No single nucleotide polymorphism was detected in four goat breeds. This result indicated that the detected loci of PRL gene had no significant effect on prolificacy of Jining Grey goats.

Key words: goat; prolificacy; prolactin gene; PCR-SSCP

CLC Number:

Q754

ZHAO Xiuhua;CHU Mingxing;WANG Jinyu;FANG Li;YE Sucheng. PCR-SSCP Analysis of Prolactin Gene in Goats[J]. , 2007, 34(11): 35-38.

[1]	SUN Qingyi, LI Mengqi, ZHAO Wei, ZHANG Wentao, ZHANG Xiaosheng, ZHANG Jinlong, ZHANG Hongjun, FANG Xiaohuan, TAO Chenyu, XIA Wei, LI Junjie. Establishment and Cryopreservation of Chengde Hornless Goat Ear Fibroblast Cell Line [J]. China Animal Husbandry and Veterinary Medicine, 2024, 51(1): 183-192.
[2]	BAI Jingjing, LIU Xiaoyu, LIU Lina, SONG Yanfeng, ZHANG Xin, SONG Xiaoyue, SHI Lei, LI Longping, ZHANG Lei, ZHU Haijing, QU Lei. Polymorphism of Notch2 Gene and Its Association Analysis with Cashmere and Growth Traits in Shaanbei White Cashmere Goats [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(9): 3630-3640.
[3]	LYU Chunrong, LIANG Jiachong, SHAO Qingyong, WU Guoquan, ZHANG Yumei, QUAN Guobo. Effects of Sodium Pyruvate on the Quality of Frozen Goat Sperm [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2363-2369.
[4]	SONG Xingchao, MENG Jinzhu, ZHAO Yuanyuan, WU Zhenyang, AN Qingming. Cloning and Bioinformatics Analysis of MyoG Gene Promoter Region Sequence in Guizhou White Goat [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(3): 893-903.
[5]	Sachula, WU Tiecheng, MA Yuejun, HE Yunmei, ZHU Lixian, HE Tuoya, WU Yuping, LIU Bin. Polymorphisms of Four Polyembryony Related Genes and Its Association Analysis with Litter Size in Cashmere Goats [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(3): 1037-1047.
[6]	WEI Yirong, ZHENG Zihua, ZHANG Sanbao, SONG Ying, JIANG Haiyu, SUN Wenyue, CHENG Pengfei, LIU Yufan, ZOU Jianwei, HUANG Yanna, PAN Yan, JIANG Qinyang. Cloning,Bioinformatics Analysis and Tissue Expression of UCP1 Gene in Nubian Goats [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(2): 440-450.
[7]	QIN Zhiyun, JIANG Huaizhi. An Overview of Research on Skin Hair Follicle Development and Regulatory Mechanism in Sheep During the Fetal Period [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(2): 638-646.
[8]	JIA Chunyan, SUN Yanyong, BAO Yonghong, ZHANG Wenguang. Bioinformatics Analysis of mRNA and lncRNA Gene Clusters and Key Signal Pathways Regulating Cashmere Growth [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(11): 4311-4324.
[9]	LI Xubo, JIANG Junming, HE Meirong, LI Haoyang, LI Hong, CHEN Si, CHEN Qiaoling, MAN Churiga, DU Li, WANG Fengyang, GAO Hongyan. Isolation,Identification of Klebsiella oxytoca from Goat and Transcriptomics Study on Infected Mice [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(10): 3906-3916.
[10]	DAN Yixin, XIANG Hua, ZHANG Huanrong, YANG Lu, REN Yupeng, XU Songwei, HE Honghong, ZHU Jiangjiang. Screening of Host Proteins Interacting with ORFV129 Protein and Cloning and Analysis of C1QBP Gene [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(1): 1-14.
[11]	PAN Siyao, ZHANG Dandan, LIU Quanhui, WANG Guodong, LYU Danwei, HUANG Ben. Effects of Inhibition of miR-142-3p on Lactation Function of Chemical Induced Mammary Epithelial Cells in Dairy Goats [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(1): 58-66.
[12]	LIU Zelin, YANG Juan, XU Mingzhu, ZHANG Nian, XIONG Qi, LIU Jingbo, TAO Hu. Association Analysis Between Polymorphisms of ZBP1 Gene and Lambing Performance in Goats [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(1): 161-173.
[13]	LONG Anju, YANG Suan, AI Jinxin, WENG Jimei, ZHANG Yiyu, CAI Huifen, LUO Weixing. Polymorphism of MC4R Gene and Its Association with Growth Traits in Qianbei Ma Goats [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(1): 198-209.
[14]	WANG Guang, ZOU Jiahao, ZHANG Yongtao, LI Dexian, LI Xueqing, YU Mengqi, CHEN Lu, YUAN Yuxin, LI Guang. Difference Analysis of Metabolites in Testis of Guanzhong Dairy Goats at Different Developmental Stages [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(9): 3453-3464.
[15]	FENG Xiaopin, LI Yinxia, ZHANG Chenjian, ZHANG Jun, MENG Chunhua, QIAN Yong, CAO Shaoxian. Coning and Polymorphism Identification of PLAG1 Gene and Its Association Analysis with Birth Weight and Body Size Traits in Boer Goats [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(9): 3465-3474.

PCR-SSCP Analysis of Prolactin Gene in Goats

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments