热应激对海南黑山羊精液抗氧化能力及精浆代谢组学特征的影响

doi:10.16431/j.cnki.1671-7236.2025.03.021

Abstract

Abstract: 【Objective】 The purpose of this experiment was to investigate the alterations in the antioxidant capacity of semen in Hainan Black goats under heat stress,and screen the potential biomarkers in seminal plasma.【Method】 Simulated in vitro heat stress treatment,the semen of Hainan Black goats was collected,and the samples were randomly divided into control group (Con) and heat stress group (HS),and the antioxidant indices of semen were determined.Additionally,non-targeted metabolome analysis of the seminal plasma was conducted using liquid chromatography-mass spectrometry,the differential metabolites were screened,and KEGG pathway enrichment analysis was carried out.【Result】 Compared with control group,the total antioxidant capacity (T-AOC) and the activity of glutathione peroxidase (GSH-Px) in semen of Hainan Black goats in heat stress group was significantly or extremely significantly decreased (P＜0.05 or P＜0.01),and the contents of reactive oxygen species (ROS) and malondialdehyde (MDA) were significantly or extremely significantly increased (P＜0.05 or P＜0.01),mitochondrial membrane potential (MMP) and adenosine triphosphate (ATP) concentration were significantly decreased (P＜0.05).The results of orthogonal partial least square discriminant analysis (OPLS-DA) indicated that there were significant variations in seminal plasma metabolism of Hainan Black goats in heat stress group,and the cluster analysis demonstrated that there was significant differences and distinct clustering between the two groups.A total of 64 distinct metabolites were identified.Among them,2-deoxyribose 5-phosphate,malonic acid,D-arginine,methyldopa and niacinamide mononucleotides in seminal plasma might respond to heat stress via pyrimidine metabolism,β-alanine metabolism,D-amino acid metabolism,niacin and niacinamide metabolism.【Conclusion】 Heat stress was significantly decreased the semen quality and antioxidant capacity of Hainan Black goats.Malonic acid,D-arginine,methyldopa acid and niacinamide mononucleotides in seminal plasma were served as potential biomarkers of the heat stress state in semen of Hainan Black goats.

Key words: Hainan Black goats; heat stress; semen; metabolomics; biomarker

CLC Number:

S827.3

AN Zhaoxiang, XUN Wenjuan, ZHOU Hanlin, SHI Liguang. Effects of Heat Stress on Semen Antioxidant Capacity and Seminal Plasma Metabolomics in Hainan Black Goats[J]. China Animal Husbandry and Veterinary Medicine, 2025, 52(3): 1191-1201.

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Effects of Heat Stress on Semen Antioxidant Capacity and Seminal Plasma Metabolomics in Hainan Black Goats

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