China Animal Husbandry and Veterinary Medicine ›› 2024, Vol. 51 ›› Issue (2): 639-648.doi: 10.16431/j.cnki.1671-7236.2024.02.020

• Genetics and Breeding • Previous Articles     Next Articles

Advances in Boar Semen Cryopreservation Technologies

WANG Jialun1, CHEN Hui2, SUN Haowei1, GUO Yaoyao1, LIANG Guodong3, MA Yunhui3, GAO Lei1   

  1. 1. College of Animal Science and Technology, Northwest A&F University, Yangling 712100, China;
    2. Shaanxi Animal Husbandry Industry Experiment and Demonstration Center, Xianyang 713702, China;
    3. Dali Country Zhongkang Livestock Breeding Co., Ltd., Weinan 715103, China
  • Received:2023-06-20 Online:2024-02-05 Published:2024-01-29
  • Contact: 国家生猪产业技术体系项目(CARS-35-PIG)
  • Supported by:
    The project was supported by the National Key Research and Development Program of China (2019YFC1905301);National Natural Science Foundation of China (22078115,21776108,21690083,22008078).

Abstract: Boar semen cryopreservation, as a vital technology for swine industry, is conducive to improve the utilization rate of excellent breeding boars and realize the long-term preservation of superior germplasm resources, as well as the elevation of efficiency in breeding farms.At present, various progress has been made in boar semen cryopreservation, the conception rate and the number born alive by domestic application of pig frozen semen have basically reached the level of foreign frozen semen breeding.However, due to the certain degree of damage of pig spermatozoa in frozen-thawed process which caused sperm fertility declined and the fertilization window period shortened, hence there is still a big gap in application of frozen semen.The article reviews the development process of cryopreservation technology for pig semen at home and abroad, introduces the basic characteristics of pig semen and sperm, briefly describes the mechanism of sperm damage caused by the cooling process, and analyzes and introduces the mechanism and methods of reducing freezing damage from the perspective of the composition of cryodiluents, such as adding cryoprotectants to reduce the formation of ice crystals inside and outside cells, maintaining osmotic pressure inside and outside cells, and stabilizing cell membrane structure.Adding antibacterial substances to inhibit the rapid reproduction of bacteria after thawing.Adding antioxidants to reduce reactive oxygen species levels, alleviate oxidative stress, protect sperm structure and fertilization ability, etc.Finally, the future development direction of boar semen cryopreservation technology is expected to provide reference for related research and practical applications.

Key words: boar; semen; cryopreservation

CLC Number: