热应激对反刍动物瘤胃功能及潜在耐热标志物的影响

doi:10.16431/j.cnki.1671-7236.2024.03.015

Abstract

Abstract: Heat stress is one of the important factors affecting animal health and production,and rumen function is closely related to nutrient absorption,body health and production performance of ruminants.Heat stress can easily cause changes in rumen microbial structure,rumen epithelial structure and barrier function,which in turn affects rumen function in ruminants.Current studies have shown that some specific rumen microorganisms,metabolites,and rumen epithelial protein expression in the rumen can be used as potential markers to evaluate the magnitude of heat stress in ruminants.By screening the potential heat tolerance markers of rumen and accurately judging the degree of heat stress in the rumen,it is helpful to better understand the mechanism of heat resistance in ruminants,and is also of great significance for the study of rumen microbial structure,rumen metabolism and rumen function.Therefore,the authors mainly review the effects of heat stress on rumen microbial structure,rumen function and potential heat tolerance markers of rumen,so as to provide theoretical basis and guidance for practical production and application under heat stress conditions.

Key words: heat stress; ruminants; rumen microorganisms; rumen function; heat resistance markers

CLC Number:

S823

ZHENG Jieyi, YANG Shuli, ZHAO Kailing, GONG Li, LI Chuan. Effects of Heat Stress on Rumen Function and Potential Heat Tolerance Markers in Ruminants[J]. China Animal Husbandry and Veterinary Medicine, 2024, 51(3): 1041-1049.

References

[1] 郑月.锦江牛与西门塔尔牛耐热性和脂质代谢及肉质的比较研究[D].南京:南京农业大学, 2017. ZHENG Y.Comparative study on the heat-tolerance and lipid metabolism and meat quality between Jingjiang and Simmental beef[D].Nanjing:Nanjing Agricultural University, 2017.(in Chinese)
[2] BELLAGI R, MARTIN B, CHASSAING C, et al.Evaluation of heat stress on tarentaise and Holstein cow performance in the mediterranean climate[J].International Journal of Biometeorology, 2017, 61(8):1371-1379.
[3] 房昊源.夏季横向交互式送风系统对钟楼式棚舍环境及西门塔尔牛生产性能和机体代谢的影响[D].南京:南京农业大学, 2020. FANG H Y.Impact of cross ventilation in summer on the environment of bell tower cowshed and the production performance, body metabolism of Simmental cattle[D].Nanjing:Nanjing Agricultural University, 2020.(in Chinese)
[4] SEJIAN V, BHATTA R, GAUGHAN J B, et al.Review:Adaptation of animals to heat stress[J].Animal, 2018, 12(s2):s431-s444.
[5] DE RENSIS F, GARCIA-ISPIERTO I, LOPEZ-GATIUS F.Seasonal heat stress:Clinical implications and hormone treatments for the fertility of dairy cows[J].Theriogenology, 2015, 84(5):659-666.
[6] MOST M S, YATES D T.Inflammatory mediation of heat stress-induced growth deficits in livestock and its potential role as a target for nutritional interventions:A review[J].Animals, 2021, 11(12):3539.
[7] MCMANUS C M, FARIA D A, LUCCI C M, et al.Heat stress effects on sheep:Are hair sheep more heat resistant?[J].Theriogenology, 2020, 155:157-167.
[8] ELLIS J L, KEBREAB E, ODONGO N E, et al.Prediction of methane production from dairy and beef cattle[J].Journal of Dairy Science, 2007, 90(7):3456-3466.
[9] BERNABUCCI U, LACETERA N, DANIELI P P, et al.Influence of different periods of exposure to hot environment on rumen function and diet digestibility in sheep[J].International Journal of Biometeorology, 2009, 53(5):387-395.
[10] ZHONG S, DING Y, WANG Y, et al.Temperature and humidity index (THI)-induced rumen bacterial community changes in goats[J].Applied Microbiology and Biotechnology, 2019, 103(7):3193-3203.
[11] MIN L, ZHAO S, TIAN H, et al.Metabolic responses and "omics" technologies for elucidating the effects of heat stress in dairy cows[J].International Journal of Biometeorology, 2017, 61(6):1149-1158.
[12] 侯宇.不同持续时间热应激对奶牛生产性能的影响及热应激预警模型的建立[D].杨凌:西北科技农林大学, 2021. HOU Y.Effect of heat stress of different duration on the production performance of cows and the establishment of warning model of heat stress[D].Yangling:Northwest A&F University, 2017.(in Chinese)
[13] 张灿.湿热应激对藏绵羊和山羊生产性能、瘤胃发酵及血液生化指标影响的比较研究[D].雅安:四川农业大学, 2016. ZHANG C.Comparative study of performance, rumen fermentaion and blood biochemical indices of Tibetan sheep (Ovis Aries) and goat under mosit-heat stress[D].Ya'an:Sichuan Agricultural University, 2016.(in Chinese)
[14] SILANIKOVE N.Effects of heat stress on the welfare of extensively managed domestic ruminants[J].Livestock Production Science, 2000, 67(1-2):1-18.
[15] UYENO Y, SEKIGUCHI Y, TAJIMA K, et al.An rRNA-based analysis for evaluating the effect of heat stress on the rumen microbial composition of Holstein heifers[J].Anaerobe, 2010, 16(1):27-33.
[16] 李子健.不同生理阶段奶牛瘤胃细菌菌群数量与多样性的比较研究[D].呼和浩特:内蒙古农业大学, 2018. LI Z J.Comparative study on rumen bacteria quantity and diversity of daily cows in different physiological stages[D].Hohhot:Inner Mongolia Agricultural University, 2018.(in Chinese)
[17] CAI L Y, YU J K, HARTANTO R, et al.Effects of heat challenge on growth performance, ruminal, blood and physiological parameters of Chinese crossbred goats[J].Small Ruminant Research, 2019, 174:125-130.
[18] GAO S T, GUO J, QUAN S Y, et al.The effects of heat stress on protein metabolism in lactating Holstein cows[J].Journal of Dairy Science, 2017, 100(6):5040-5049.
[19] 王哲奇,徐元庆,石璐璐,等.热应激对反刍动物采食和瘤胃功能的影响[J].动物营养学报, 2019, 3(8):3448-3455. WANG Z Q, XU Y Q, SHI L L, et al.Effect of heat stress on feed intake and rumen function of ruminants[J].Chinese Journal of Animal Nutrition, 2019, 3(8):3448-3455.(in Chinese)
[20] 钟书.温湿度指数(THI)介导山羊瘤胃细菌群落的变化[D].杨凌:西北科技农林大学, 2019. ZHONG S.Temperature and humidity index (THI) induced rumen bacterial community changes in goats[D].Yangling:Northwest A&F University, 2019.(in Chinese)
[21] 蔡丽媛.集约化羊舍的环境控制及热应激对山羊瘤胃发酵的影响[D].武汉:华中农业大学, 2015. CAI L Y.Environmental control of intensive goat buildings and the effect of heat stress on rumen fermentation of goats[D].Wuhan:Huangzhong Agricultural University, 2015.(in Chinese)
[22] 刘爱瑜,王超,吴占军,等.热应激对断奶绵羔羊生长性能、抗氧化性能和瘤胃菌群的影响[J].草业学报, 2023, 32(4):173-182. LIU A Y, WANG C, WU Z J, et al.Impact of heat stress on growth rate, serum antioxidant properties, and rumen flora in weaned lambs[J]. Acta Prataculturae Sinica, 2023, 32(4):173-182.(in Chinese)
[23] TAJIMA K, NONAKA I, HIGUCHI K, et al.Influence of high temperature and humidity on rumen bacterial diversity in Holstein heifers[J].Anaerobe, 2007, 13(2):57-64.
[24] CORREIA SALES G F, CARVALHO B F, SCHWAN R F, et al.Heat stress influence the microbiota and organic acids concentration in beef cattle rumen[J].Journal of Thermal Biology, 2021, 97:102897.
[25] 周熊艳,王松明,顾招兵,等.云南大额牛瘤胃微生物多样性分析[J].中国畜牧兽医, 2016, 43(5):1162-1168. ZHOU X Y, WANG S M, GU Z B, et al.Diversity analysis of rumen microbes in Yunnan gayals (Bos frontalis)[J]. China Animal Husbandry&Veterinary Medicine, 2016, 43(5):1162-1168.(in Chinese)
[26] RUSSELL J B, MUCK R E, WEIMER P J.Quantitative analysis of cellulose degradation and growth of cellulolytic bacteria in the rumen[J]. FEMS Microbiology Ecology, 2009, 67(2):183-197.
[27] ZHANG L, CHUNG J, JIANG Q, et al.Characteristics of rumen microorganisms involved in anaerobic degradation of cellulose at various pH values[J].Rsc Advances, 2017, 7(64):40303-40310.
[28] CHAIDANYA K, SOREN N M, SEJIAN V, et al.Impact of heat stress, nutritional stress and combined (heat and nutritional) stresses on rumen associated fermentation characteristics, histopathology and HSP70 gene expression in goats[J].Journal of Animal Behaviour and Biometeorology, 2017, 5(2):36-48.
[29] BAEK Y C, CHOI H, JEONG J Y, et al.The impact of short-term acute heat stress on the rumen microbiome of Hanwoo steers[J].Journal of Animal Science and Technology, 2020, 62(2):208-217.
[30] LIU E, SUN M, HE C, et al.Rumen microbial metabolic responses of dairy cows to the honeycomb flavonoids supplement under heat-stress conditions[J].Frontiers in Veterinary Science, 2022, 9:845911.
[31] 杜瑞平,温雅俐,姚焰础,等.热应激对奶牛瘤胃液微生物数量的影响[J].动物营养学报, 2013, 25(2):334-343. DU R P, WEN Y L, YAO Y C, et al.Effect of heast stress on microbial number in rumen fluid of dairy cow[J].Chinese Journal of Animal Nutrition, 2013, 25(2):334-343.(in Chinese)
[32] 王建平,王加启,卜登攀,等.热应激对奶牛瘤胃纤维分解菌的影响[J].农业生物技术学报, 2010, 18(2):302-307. WANG J P, WANG J Q, BU D P, et al.Effect of heat stress on quantities of ruminal cellulytic bacteria in dairy cattle[J].Journal of Agricultural Biotechnology, 2010, 18(2):302-307.(in Chinese)
[33] WANG Z, ELEKWACHI C O, JIAO J, et al.Investigation and manipulation of metabolically active methanogen community composition during rumen development in Black goats[J].Scientific Reports, 2017, 7(1):422.
[34] 董利锋,付敏,陈天宝,等.反刍动物瘤胃优势产甲烷菌菌群结构及多样性研究进展[J].动物营养学报, 2019, 31(9):3927-3935. DONG L F, FU M, CHEN T B, et al.Research progress on dominant methanogen composition and diversity in rumen of ruminants[J]. Chinese Journal of Animal Nutrition, 2019, 31(9):3927-3935.(in Chinese)
[35] 淡瑞芳,张海涛,龙瑞军,等.藏系绵羊瘤胃细菌数量及其放牧地牧草养分随季节变化的研究[J].草业学报, 2009, 18(1):100-104. DAN R F, ZHANG H T, LONG R J, et al.Seasonal shift of rumen bacteria quantity of grazing Tibetan sheep and forage nutrition by grazing sheep[J].Acta Prataculturae Sinica, 2009, 18(1):100-104.(in Chinese)
[36] 周旭.热应激对奶牛泌乳性能和瘤胃产甲烷菌群的影响[D].乌鲁木齐:新疆农业大学, 2018. ZHOU X.Effect of heat stress on milk performence and ruminal methanogen population in dairy cows[D].Urumqi:Xinjiang Agricultural University, 2018.(in Chinese)
[37] 闵力.基于生理代谢、蛋白组学和菌群多样性解析热应激对泌乳奶牛的影响[D].北京:中国农业大学, 2017. MIN L.Base on metabolic response, proteomics and bacterical diversity for elucidating the effects of heat stress in lactating dairy cows[D].Beijing:China Agricultural University, 2017.(in Chinese)
[38] ZHAO S G, MIN L, ZHENG N, et al.Effect of heat stress on bacterial composition and metabolism in the rumen of lactating dairy cows[J].Animals, 2019, 9(11):925.
[39] VIEIRA SALLES M S, ZANETTI M A, SALLES F A, et al.Changes in ruminal fermentation and mineral serum level in animals kept in high temperature environments[J].Revista Brasileira de Zootechia-Brazilian Journal of Animal Science, 2010, 39(4):883-890.
[40] AMMER S, LAMBERTZ C, VON SOOSTEN D, et al.Impact of diet composition and temperature-humidity index on water and dry matter intake of high-yielding dairy cows[J].Journal of Animal Physiology and Animal Nutrition, 2018, 102(1):103-113.
[41] 王雪莹,王之盛,薛白,等.烟酸对热应激牦牛生长性能、营养物质表观消化率和血液指标的影响[J].动物营养学报, 2020, 32(5):2228-2240. WANG X Y, WANG Z S, XUE B, et al.Effect of niacin on growth performance, nutrient apparent digestibility and blood indexes of yak under heat-stress[J].Chinese Journal of Animal Nutrition, 2020, 32(5):2228-2240.(in Chinese)
[42] PRAGNA P, SEJIAN V, SOREN N M, et al.Summer season induced rhythmic alterations in metabolic activities to adapt to heat stress in three indigenous (Osmanabadi, Malabari and Salem Black) goat breeds[J].Biological Rhythm Research, 2018, 49(4):551-565.
[43] 许啸,刘君地,李燕,等.热应激对奶山羊瘤胃发酵指标的影响及有机铬对其的调控作用[J].动物营养学报, 2013, 25(1):100-106. XU X, LIU J D, LI Y, et al.Effect of heat stress on rumen fermentation indexes of dairy goats and regulation function of organic chromium[J].Chinese Journal of Animal Nutrition, 2013, 25(1):100-106.(in Chinese)
[44] 马燕芬,陈琦,杜瑞平,等.热应激对奶山羊瘤胃上皮细胞屏障通透性的影响[J].中国农业科学, 2013, 46(21):4478-4485. MA Y F, CHEN Q, DU R P, et al.Effect of heat stress on rumen epithelial cell barrier permeability in dairy goat[J].Scientia Agricultura Sinica, 2013, 46(21):4478-4485.(in Chinese)
[45] 黄帆,吕秋凤.热应激诱导的氧化应激对动物肠道组织的损伤[J].动物营养学报, 2017, 29(6):1856-1860. HUANG F, LYU Q F.Oxidative stress induced by heat stress:Injury on intestinal tissue of animals[J].Chinese Journal of Animal Nutrition, 2017, 29(6):1856-1860.(in Chinese)
[46] YU Z, CANTET J M, PAZ H A, et al.Heat stress-associated changes in the intestinal barrier, inflammatory signals, and microbiome communities in dairy calves[J].Journal of Dairy Science, 2023, Doi:10.3168/jds.2023-23873.Epub ahead of print.
[47] YAZDI M H, MIRZAEI-ALAMOUTI H R, AMANLOU H, et al.Effects of heat stress on metabolism, digestibility, and rumen epithelial characteristics in growing Holstein calves[J].Journal of Animal Science, 2016, 94(1):77-89.
[48] 柳雷振.热应激对西门塔尔种公牛的影响及耐热性相关瘤胃微生物和代谢物的研究[D].郑州:河南农业大学, 2022. LIU L Z.Effect of heat stress on Simmental bulls and studies on heat tolerance-related rumen microbes and metabolites[D].Zhengzhou:Henan Agricultural University, 2022.(in Chinese)
[49] 谢颖.不同环境温度对奶牛血清相关指标及瘤胃微生物的影响[D].南京:南京农业大学, 2020. XIE Y.Effect of the difference environmental temperature on serum relatend indexes and rumen microflora in cows[D].Nanjing:Nanjing Agricultural University, 2017.(in Chinese)
[50] SAAD A H, AHMED M S, ABOUBAKR M, et al.Impact of dietary or drinking water Ruminococcus sp.supplementation and/or heat stress on growth, histopathology, and bursal gene expression of broilers[J].Fronties in Veterinary Science, 2021, 8:663577.
[51] 涂瑾秋,陈代文,郑萍.乳酸对炎症的调节作用研究进展[J].动物营养学报, 2023, 35(8):4860-4871. TU J Q, CHEN D W, ZHENG P.Research progress in regulation of inflammation by lactic acid[J].Chinese Journal of Animal Nutrition, 2023, 35(8):4860-4871.(in Chinese)
[52] TIAN H, WANG W, ZHENG N, et al.Identification of diagnostic biomarkers and metabolic pathway shifts of heat-stressed lactating dairy cows[J].Journal of Proteomics, 2015, 125:17-28.
[53] TIAN H, ZHENG N, WANG W, et al.Integrated metabolomics study of the milk of heat-stressed lactating dairy cows[J].Scientific Reports, 2016, 6:24208.
[54] 樊欣,彭仁.热休克蛋白70:生物学功能与作用机制研究进展[J].生命科学, 2019, 31(3):270-278. FAN X PENG R.Heat shock protein 70:Biological function and the mechanism for its function[J].Chinese Bulletin of Life Sciences, 2019, 31(3):270-278.(in Chinese)
[55] PARMAR M S, MADAN A K, HUOZHA R, et al.Heat shock protein 70(HSP70) gene expression pattern in peripheral blood mononuclear cells (PBMCs) during different seasons in Sahiwal cows (Bos indicus)[J].Journal of Animal Research, 2015, 5(1):109.
[56] MIN L, CHENG J-B, SHI B-L, et al.Effects of heat stress on serum insulin, adipokines, AMP-activated protein kinase, and heat shock signal molecules in dairy cows[J].Journal of Zhejiang University-Science B, 2015, 16(6):541-548.
[57] 杨晓晴,崔燕,何俊峰,等.热休克蛋白70在牦牛胃中的分布和表达[J].动物营养学报, 2018, 30(7):2626-2631. YANG X Q, CUI Y, HE J F, et al.Ditribution and expression of heat shock protein 70 in stomachs of yaks[J].Chinese Journal of Animal Nutrition, 2018, 30(7):2626-2631.(in Chinese)
[58] 吕卫兵,牛彬,沙玉柱,等.放牧藏绵羊瘤胃热休克蛋白70家族基因表达特征及其与微生物的互作研究[J].西北农业学报, 2022, 31(12):1535-1543. LYU W B, NIU B, SHA Y Z, et al.Expression characteristics of rumen heat shock protein 70 family genes in grazing Tibetan sheep and its interaction with microorganisms[J].Acta Agriculturae Boreali-occidentalis Sinica, 2022, 31(12):1535-1543.(in Chinese)
[59] 王哲奇.温热环境对绵羊增重性能及生理指标影响的研究[D].呼和浩特:内蒙古农业大学, 2020. WANG Z Q.Study on effect of thermal environment on weight gain performance and physiological indexes of sheep[D].Hohhot:Inner Mongolia Agricultural University, 2020.(in Chinese)
[60] KIM W S, GHASSEMI NEJAD J, ROH S G, et al.Heat-shock proteins gene expression in peripheral blood mononuclear cells as an indicator of heat stress in beef calves[J].Animals (Basel), 2020, 10(5):895.
[61] JEWELL K A, MCCORMICK C A, ODT C L, et al.Ruminal bacterial community composition in dairy cows is dynamic over the course of two lactations and correlates with feed efficiency[J].Applied and Environmental Microbiology, 2015, 81(14):4697-4710.
[62] 彭孝坤,张宇,黄晓瑜,等.急性冷应激对绵羊免疫功能和不同组织热休克蛋白70家族基因表达的影响[J].畜牧兽医学报, 2019, 50(8):1625-1634. PENG X K, ZHANG Y, HUANG X Y, et al.Effect of acute cold stress on immune function and expression of heat shock protein 70 family genes in different tissuse in sheep[J].Acta Veterinaria et Zootechnica Sinica, 2019, 50(8):1625-1634.(in Chinese)

Effects of Heat Stress on Rumen Function and Potential Heat Tolerance Markers in Ruminants

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