牛瘤胃微生物的分离鉴定与生化特性分析

doi:10.16431/j.cnki.1671-7236.2022.05.017

Abstract

Abstract: 【Objective】 In this experiment,the strains isolated from the rumen juice were studied to provide basic data for the preparation of probiotic preparations.【Method】 The rumen juice of 10 healthy Holstein dairy cattle was collected,and a single strain was obtained by coating,characteristic culture and purification.The bacterial DNA was extracted and amplified by 16S rDNA PCR.After 16S rDNA gene sequencing and identification,sequence alignment was carried out and evolutionary tree was constructed to determine the species of strains.Different kinds of bacteria were cultured for 0-48 hours to determine their growth characteristics,and acid,alkali and bile salt tolerance experiments were carried out to determine their different tolerance.【Result】 20 strains were obtained by coating the isolated strains.After PCR amplification and sequencing,it was found that 20 strains belonged to 7 different bacteria.It was roughly determined that L7 was Bacillus amyloliquefaciens,K7 was Bacillus subtilis,S7 was Stenotrophomonas maltophilia,C2 was Bacillus licheniformis,M7 was Streptococcus equi,F7 was Citrobacter freundii,and T7 was Kurt gigasii.From the growth curve,it could be seen that the optimum growth time of the seven strains reached the fastest growth period in 24-36 h respectively.M7 and K7 had strong tolerance to acid,while S7,C2 and F7 were more sensitive to acid,C2 had the strongest tolerance to alkali,T7 had the strongest tolerance to bile salt,and S7 had the least tolerance to bile salt.【Conclusion】 The seven strains isolated from rumen in this study had different degrees of tolerance to acid-base and bile salt,and the optimal growth cycle.

Key words: Holstein dairy cattle; rumen microorganisms; biochemical characteristics; growth curve

CLC Number:

S811.6

LI Changyang, GAO Bingnan, REN Yulong, ZHANG Hao, SUN Yu, WEI Xiao, WANG Qiuju. Isolation,Identification and Biochemical Characteristics Analysis of Bovine Rumen Functional Bacteria[J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(5): 1757-1764.

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Isolation,Identification and Biochemical Characteristics Analysis of Bovine Rumen Functional Bacteria

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