公猪精液质量与精子脂质成分相关性研究

doi:10.16431/j.cnki.1671-7236.2024.04.022

Abstract

Abstract: 【Objective】 The purpose of this experiment was to establish the correlation between boar semen quality and sperm lipid composition,providing support for accurate evaluation of boar semen quality.【Method】 In this study,semen samples were collected from representative breeds of boars,such as Yorkshire pigs and Landrace pigs.The computer-assisted sperm analysis (CASA) system was used to measure semen quality parameters,including sperm density,sperm motility,sperm abnormality rate,and semen volume.The semen samples were classified as two groups based on sperm motility,including normal motility (≥70%,n=11) and low motility (＜70%,n=11).Unbiased lipidomics analysis of the two groups of sperm samples was performed using UPLC-Q-Exactive-Orbitrap/MS multivariate statistical analysis methods,including t tests,partial least squares discriminant analysis (PLS-DA),and correlation analysis,were used to analyze the data of lipidomics.The difference of lipid molecules between the two groups was obtained according to VIP＞1 and P＜0.05.【Result】 A total of 166 differential lipid molecules identified in both positive and negative ion modes,which mainly concentrated in phosphatidylcholine (PC).Lipid molecular species,including diglyceride(DG),sphingomyelin(SM),PC,Zymosterol Ester (ZyE),and simple Glc series (Hex3Cer),showed significant decrease in low motility sperm and were significantly positively correlated with sperm motility (P＜0.05).Triglyceride (TG) and phosphatidylcholine ester (PC(e)) showed significant increase in low motility sperm and were significantly negatively correlated with sperm motility(P＜0.05).【Conclusion】 Lipidomics could accurately distinguish the differences in lipid subpopulations and lipid molecular composition among different motile sperm.The composition and content of lipids such as PC,DG,SM,PC,ZyE,Hex3Cer,TG,and PC(e) could be used as markers to distinguish sperm motility.

Key words: boars; semen quality; lipid composition; lipidomics

CLC Number:

S814.1

CHEN Jiaying, MA Bowen, ZHAO Qingyu, ZHANG Junmin, TANG Chaohua. Correlation Study Between Semen Quality and Sperm Lipid Composition in Boars[J]. China Animal Husbandry and Veterinary Medicine, 2024, 51(4): 1537-1548.

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Correlation Study Between Semen Quality and Sperm Lipid Composition in Boars

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