热应激对山羊粪便菌群结构及代谢产物的影响

doi:10.16431/j.cnki.1671-7236.2024.08.007

Abstract

Abstract: 【Objective】 The aim of this trial was to investigate the effects of heat stress on fecal flora structure and metabolites in goats.【Method】 Six adult castrated rams of Dazu Montenegrin sheep with similar age and body weight of 28.4 kg±3.2 kg were selected,and a single factor self-control experimental design was used.They were subjected to three stages of treatment:Severe heat stress (H group,temperature and humidity index (THI)=91),moderate heat stress (M group,THI=84),and no heat stress (L group,THI=71).Each treatment stage was 15 days,and the transition period was 7 days.Rectal feces were collected at 14:00 on the last day of each stage for microbial 16S rRNA sequencing and metabolomics analysis.【Result】 There was no significant difference in the diversity of goat fecal flora among the three groups (P＞0.05).Compared with group L,in H and M groups,the relative abundance of Bacteroidetes was significantly decreased (P＜0.05),and the relative abundance of Proteobacteria was significantly increased (P＜0.05). The relative abundances of Christensenaceae R-7 group,Clostridium sensu stricto 1,and Family XⅢ AD3011 group were significantly increased (P＜0.05),whereas the relative abundances of Bacteroides and unclassified_f_Lachnospiraceae were significantly decreased (P＜0.05). The contents of atrazine,cholic acid,taurocholate,oleic acid,hyperforin,and taurocholic acid in feces were significantly increased (P＜0.05),whereas the contents of Pro-Ala,Arg-Thr and homoveratric acid were significantly decreased (P＜0.05).Differential metabolites in feces under heat stress conditions were significantly enriched in the pathways of taurine and hypotaurine metabolism,ABC transporters,glutathione metabolism and gap junction (P＜0.05).【Conclusion】 Heat stress led to changes in the structure of goat fecal flora,which was not conducive to goat intestinal health. It also upregulated the biosynthetic metabolic pathways of arginine and primary bile acids,which helped goats adapt to high humidity and heat environments. It also led to an increase in the content of toxic substances in goat metabolites,which might cause damage to the intestinal barrier and nervous system.

Key words: goat; heat stress; fecal flora structure; metabolites

CLC Number:

S826

WANG Changtong, YANG Liandi, YIN Li, WANG Le, ZHANG Jin, ZUO Fuyuan, HUANG Wenming. Effects of Heat Stress on the Structure and Metabolites of Fecal Flora of Goats[J]. China Animal Husbandry and Veterinary Medicine, 2024, 51(8): 3288-3300.

References

[1] 王哲奇,徐元庆,史彬林,等.热应激状态下羊的体温调节[J].动物营养学报,2017,29(7):2211-2219. WANG Z Q,XU Y Q,SHI B L,et al.Heat stress and thermoregulation of sheep and goats[J]. Chinese Journal of Animal Nutrition,2017,29(7):2211-2219.(in Chinese)
[2] MAURYA V P,SEJIAN V,KUMAR D,et al.Impact of heat stress,nutritional restriction and combined stresses (heat and nutritional) on growth and reproductive performance of Malpura rams under semi-arid tropical environment[J].Journal of Animal Physiology and Animal Nutrition,2016,100(5):938-946.
[3] BAGATH M,KRISHNAN G,DEVARAJ C,et al.The impact of heat stress on the immune system in dairy cattle:A review[J]. Research in Veterinary Science,2019,126:94-102.
[4] CRISTOFORI F,DARGENIO V N,DARGENIO C,et al.Anti-inflammatory and immunomodulatory effects of probiotics in gut inflammation:A door to the body[J].Frontiers in Immunology,2021,12:578386.
[5] MATTHEWS C,CRISPIE F,LEWIS E,et al.The rumen microbiome:A crucial consideration when optimising milk and meat production and nitrogen utilisation efficiency[J].Gut Microbes,2019,10(2):115-132.
[6] LAMBERT G P.Stress-induced gastrointestinal barrier dysfunction and its inflammatory effects[J].Journal of Animal Science,2009,87(14 Suppl):E101-E108.
[7] HALL D M,BUETTNER G R,OBERLEY L W,et al.Mechanisms of circulatory and intestinal barrier dysfunction during whole body hyperthermia[J].American Journal of Physiology.Heart and Circulatory Physiology,2001,280(2):H509-H521.
[8] KOCH F,THOM U,ALBRECHT E,et al.Heat stress directly impairs gut integrity and recruits distinct immune cell populations into the bovine intestine[J]. Proceedings of the National Academy of Sciences of the United States of America,2019,116(21):10333-10338.
[9] SPRAGUE J L,KASPER L,HUBE B.From intestinal colonization to systemic infections:Candida albicans translocation and dissemination[J].Gut Microbes,2022,14(1):2154548.
[10] DENG Z C,YANG J C,HUANG Y X,et al.Translocation of gut microbes to epididymal white adipose tissue drives lipid metabolism disorder under heat stress[J].Science China Life Sciences,2023,66(12):2877-2895.
[11] 陈少侃,罗汉鹏,张国兴,等.热应激条件下荷斯坦奶牛后段肠道差异微生物分析[J].中国畜牧兽医,2019,46(8):2273-2280. CHEN S K,LUO H P,ZHANG G X,et al.Analysis of differences in hindgut microbiota of Holstein dairy cows during heat stress[J].China Animal Husbandry & Veterinary Medicine,2019,46(8):2273-2280.(in Chinese)
[12] 郑卓.热应激对夏南牛生理生化、行为及直肠菌群和代谢物的影响与遮阳网的缓解效果[D].郑州:河南农业大学,2022. ZHENG Z.Effects of heat stress on physiological,biochemical and behaviour,gut microbiota and metabolites of Xianan cattle and mitigation effects of shade nets [D].Zhengzhou:Henan Agricultural University,2022.(in Chinese)
[13] CONTRERAS-JODAR A,NAYAN N H,HAMZAOUI S,et al.Heat stress modifies the lactational performances and the urinary metabolomic profile related to gastrointestinal microbiota of dairy goats[J].PLoS One,2019,14(2):e202457.
[14] M’HAMDI N,DAREJ C,ATTIA K,et al.Modelling THI effects on milk production and lactation curve parameters of Holstein dairy cows[J].Journal of Thermal Biology,2021,99:102917.
[15] MACPHERSON A J,HARRIS N L.Interactions between commensal intestinal bacteria and the immune system[J].Nature Reviews.Immunology,2004,4(6):478-485.
[16] KOLODZIEJCZYK A A,ZHENG D,ELINAV E.Diet-microbiota interactions and personalized nutrition[J]. Nature Reviews.Microbiology,2019,17(12):742-753.
[17] WOO V,ALENGHAT T.Host-microbiota interactions:Epigenomic regulation[J]. Current Opinion in Immunology,2017,44:52-60.
[18] XAVIER R J,PODOLSKY D K.Unravelling the pathogenesis of inflammatory bowel disease[J].Nature,2007,448(7152):427-434.
[19] GIBIINO G,LOPETUSO L R,SCALDAFERRI F,et al.Exploring bacteroidetes:Metabolic key points and immunological tricks of our gut commensals[J].Digestive and Liver Disease,2018,50(7):635-639.
[20] MAZMANIAN S K,ROUND J L,KASPER D L.A microbial symbiosis factor prevents intestinal inflammatory disease[J].Nature,2008,453(7195):620-625.
[21] WEXLER H M.Bacteroides:The good,the bad,and the nitty-gritty[J].Clinical Microbiology Reviews,2007,20(4):593-621.
[22] KHAFIPOUR E,LI S,PLAIZIER J C,et al.Rumen microbiome composition determined using two nutritional models of subacute ruminal acidosis[J]. Applied and Environmental Microbiology,2009,75(22):7115-7124.
[23] ZHAO C,HU X,BAO L,et al.Gut dysbiosis induces the development of mastitis through a reduction in host anti-inflammatory enzyme activity by endotoxemia[J].Microbiome,2022,10(1):205.
[24] CUESTA S,BURDISSO P,SEGEV A,et al.Gut colonization by Proteobacteria alters host metabolism and modulates cocaine neurobehavioral responses[J].Cell Host and Microbe,2022,30(11):1615-1629.
[25] 贾银海,吴思谦,朱文,等.基于16S rRNA探究应激条件下娟姗牛肠道菌群结构的调控机制[J].中国畜牧杂志,2023,59(4):75-82. JIA Y H,WU S Q,ZHU W,et al.Research on the regulation mechanism of intestinal flora structure of stress condition in Jersey using 16S rRNA[J].Chinese Journal of Animal Science,2023,59(4):75-82.(in Chinese)
[26] BARICHELLA M,SEVERGNINI M,CILIA R,et al.Unraveling gut microbiota in Parkinson’s disease and atypical parkinsonism[J].Movement Disorders,2019,34(3):396-405.
[27] HILL-BURNS E M,DEBELIUS J W,MORTON J T,et al.Parkinson’s disease and Parkinson’s disease medications have distinct signatures of the gut microbiome[J]. Movement Disorders,2017,32(5):739-749.
[28] LIU Y,LIU C,WU H,et al.Small intestine microbiome and metabolome of high and low residual feed intake angus heifers[J].Frontiers in Microbiology,2022,13:862151.
[29] LEIBOVITZH H,LEE S H,XUE M,et al.Altered gut microbiome composition and function are associated with gut barrier dysfunction in healthy relatives of patients with crohn’s disease[J]. Gastroenterology,2022,163(5):1364-1376.
[30] BAI X,SHI Y,TANG L,et al.Heat stress affects faecal microbial and metabolic alterations of rabbits[J].Frontiers in Microbiology,2021,12:817615.
[31] LI H,LI R,CHEN H,et al.Effect of different seasons (spring vs summer) on the microbiota diversity in the feces of dairy cows[J]. International Journal of Biometeorology,2020,64(3):345-354.
[32] IKEDA T,NISHIDA A,YAMANO M,et al.Short-chain fatty acid receptors and gut microbiota as therapeutic targets in metabolic,immune,and neurological diseases[J]. Pharmacology and Therapeutics,2022,239:108273.
[33] VACCA M,CELANO G,CALABRESE F M,et al.The controversial role of human gut Lachnospiraceae[J].Microorganisms,2020,8(4):573.
[34] RYAN D,PREZZA G,WESTERMANN A J.An RNA-centric view on gut bacteroidetes[J].Biological Chemistry,2020,402(1):55-72.
[35] SEJIAN V,BHATTA R,GAUGHAN J B,et al.Review:Adaptation of animals to heat stress[J].Animal,2018,12(s2):s431-s444.
[36] POPOVIC P J,ZEH H R,OCHOA J B.Arginine and immunity[J].The Journal of Nutrition,2007,137(6 Suppl2):1681S-1686S.
[37] ZHU X,PRIBIS J P,RODRIGUEZ P C,et al.The central role of arginine catabolism in T-cell dysfunction and increased susceptibility to infection after physical injury[J].Annals of Surgery,2014,259(1):171-178.
[38] ROSENTHAL M D,CARROTT P W,PATEL J,et al.Parenteral or enteral arginine supplementation safety and efficacy[J].The Journal of Nutrition,2016,146(12):2594S-2600S.
[39] XIA Z,HUANG L,YIN P,et al.L-arginine alleviates heat stress-induced intestinal epithelial barrier damage by promoting expression of tight junction proteins via the AMPK pathway[J].Molecular Biology Reports,2019,46(6):6435-6451.
[40] XU Y,LABEDAN B,GLANSDORFF N.Surprising arginine biosynthesis:A reappraisal of the enzymology and evolution of the pathway in microorganisms[J].Microbioloag and Molecular Biology Reviews,2007,71(1):36-47.
[41] 李宁,蒋昀轩,刘德凤,等.两种动物胆汁酸对热应激小鼠肝脏和空肠保护效果的比较研究[J].中国畜牧兽医,2023,50(7):2729-2739. LI Y,JIANG Y X,LIU D F,et al.Comparative study on the protective effects of two animal bile acids on liver and jejunum in heat-stressed mice[J].China Animal Husbandry & Veterinary Medicine,2023,50(7):2729-2739.(in Chinese)
[42] 陈玉艳,李静,刘鸿鹤,等.胆汁酸在畜禽及水产养殖中的应用[J].中国饲料,2024,13:15-21. CHEN Y Y,LI J,LIU H H,et al.Application of bile acids in livestock and aquaculture[J].China Feed,2024,13:15-21.(in Chinese)
[43] HOU Y,ZHANG L,DONG R Y,et al.Comparing responses of dairy cows to short-term and long-term heat stress in climate-controlled chambers[J].Journal of Dairy Science,2021,104(2):2346-2356.
[44] YANG M,GU Y,LI L,et al.Bile acid-gut microbiota axis in inflammatory bowel disease:From bench to bedside[J]. Nutrients,2021,13(9):3143.
[45] 潘文兰,李红霞,蔡梦宇,等.热应激对肠道及认知功能的影响[J].环境与职业医学,2023,40(11):1341-1346. PAN W L,LI H X,CAI M Y,et al.Effects of heat stress on intestinal tract and cognitive function[J].Journal of Environmental and Occupational Medicine,2023,40(11):1341-1346.(in Chinese)
[46] GENOVESE T,SIRACUSA R,FUSCO R,et al.Atrazine inhalation causes neuroinflammation,apoptosis and accelerating brain aging[J].International Journal of Molecular Sciences,2021,22(15):7938.
[47] LIU Y,CHENG Y,LI J,et al.Epoxy stearic acid,an oxidative product derived from oleic acid,induces cytotoxicity,oxidative stress,and apoptosis in HepG2 cells[J].Journal of Agricultural and Food Chemistry,2018,66(20):5237-5246.

Effects of Heat Stress on the Structure and Metabolites of Fecal Flora of Goats

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