›› 2007, Vol. 34 ›› Issue (11): 126-129.

• 公共卫生 • Previous Articles     Next Articles

Study on the Multi Residues of Fluoroquinolones by Enzyme Immunoassay

PAN Xiaocheng1, QI Kezong2,ZHU Liangqiang3,ZHAO Ruihong1
   

  1. 1.Institute of Animal and Veterinary Science, Anhui Academy of Agricultural Science, Hefei 230031, China;2.Anhui Agricultural University, Hefei 230036, China; 3. Anhui Veterinary Station, Hefei 230022, China 
  • Received:1900-01-01 Revised:1900-01-01 Online:2007-11-20 Published:2007-11-20

Abstract: The antiserum was prepared with the mixture of OFL-BSA,DIF-BSA and NORBSA.The Ci-ELISA method for the detection of ofloxacin ,difloxacin and norfloxacin was established with it.To ofloxacin ,difloxacin and norfloxacin.The regression equations about their standard curves were y=-0.1984x+0.9687(r=0.9885),y=-0.1738x+0.9150 (r=0.9985) and y=-0.2004x+0.9572 (r=0.9954),respectively.The 50% inhibition concentration(IC50) were 231.57,245.32 and 186.14ng/ml,respectively. The limits of detect (LOD) were 1.62,1.86 and 1.07ng/ml ,respectively. The coefficients of variation for the Ci-ELISA method were less than 5.44% in intra-assay and 12.45% in inter-assay. The recoveries in milk ranged from 71.70% to 94.53%.

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