大肠杆菌与金黄色葡萄球菌对奶牛子宫内膜组织细胞因子表达及损伤程度的影响

doi:10.16431/j.cnki.1671-7236.2019.06.033

Abstract

Abstract:

The aim of this study was to investigate the effects of Staphylococcus aureus (S.aureus) and Escherichia coli (E.coli) on the expression of cytokines interleukin-6 (IL-6),IL-1β and IL-8 and damage of dairy cow endometrial tissues.The in vitro cultured dairy cow endometrium tissue was used as the research object,and was infected in vitro with E.coli and S.aureus at a concentration of 1×10⁵ to 1×10⁹ CFU/mL.Real-time PCR and ELISA were used to detect the expression of IL-6,IL-1β and IL-8 mRNA and protein in dairy cows endometrium tissue after two kinds of bacteria stimulation,and the dairy cows endometrial histopathology sections were observed by HE staining.The results showed that the expression of IL-6,IL-1β and IL-8 mRNA in dairy cows endometrium tissue was extremely significantly higher than that in the blank control group after E.coli infection at a concentration of 1×10⁵ to 1×10⁹ CFU/mL (P<0.01).When the concentration of S.aureus infection was 1×10⁵ and 1×10⁶ CFU/mL,the expression of IL-6 and IL-1β mRNA was extremely significantly higher than that of the blank control group (P<0.01);The infection concentration was 1×10⁷ CFU/mL,the expression of IL-6 and IL-1β mRNA was significantly higher than that in the blank control group (P<0.05);The infection concentration was 1×10⁶ CFU/mL,the expression of IL-8 mRNA was significantly higher than that in the blank control group (P<0.05);Other infection concentrations were not significantly different from the blank control group (P>0.05).The expression levels of IL-6,IL-1β and IL-8 were significantly higher in the dairy cows endometrial tissues infection with 1×10⁵ to 1×10⁹ CFU/mL S.aureus and E.coli (P<0.01).The expression of IL-6,IL-1β and IL-8 mRNA and protein in the dairy cows endometrial tissues infected with E.coli at the same concentration was extremely significantly higher than those in the S.aureus infection group (P<0.01).The results of HE section staining showed that some epithelial cells remained after E.coli infection,while epithelial cells all fell off after S.aureus infection.The results of this test showed that the inflammatory response caused by E.coli and S.aureus infected with endometrial tissue of cows was different.After E.coli infection,pro-inflammatory cytokines were significantly up-regulated,while S.aureus infection destroyed endometrial epithelial cells to a greater extent.

Key words: dairy cows endometrium tissue; E.coli; S.aureus; IL-6; IL-1β; IL

CLC Number:

S852.61

LIU Kun, MAO Wei, LI Tingting, LIU Bo, GUAN Hong, PEI Le, CAO Jinshan. Effect of Escherichia coli and Staphylococcus aureus on Expression of Cytokine and Damage Degree of Dairy Cows Endometrium Tissue[J]. , 2019, 46(6): 1841-1848.

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Effect of Escherichia coli and Staphylococcus aureus on Expression of Cytokine and Damage Degree of Dairy Cows Endometrium Tissue

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