南阳牛生长性状相关基因组区域全基因组关联分析

doi:10.16431/j.cnki.1671-7236.2020.01.009

Abstract

Abstract: The aim of this study was to screen and identify the genomic regions and candidate genes related to growth traits of Nanyang cattle,so as to better understand the genetic mechanism of growth traits.Blood samples of 71 Nanyang cattle were collected and used to extract genomic DNA.The specific-locus amplified fragment sequencing (SLAF-seq) technology was used to obtain genome-wide SNP markers and classify the genotypes of experimental individuals.A genome-wide association analysis (GWAS) was carried out for several growth traits consisting of body weight (BW),body height (BH),body length (BL),chest girth (CG),ischium width (IW) and body weight gain (BWG).BW,BH,BL,CG and IW of every individual were measured in every six months from birth to 36 months of age.BWG was calculated as the difference between subsequent body weight measurements with 6-month interval.Gene annotation was performed for the genes within the identified genomic regions to prioritize candidate genes.After filtering,141 755 SNPs remained for the GWAS.5 genomic regions (LOD ≥ 6.35) significantly associated with BW at 12 months of age(chromosome 8:17 320 634-17 347 720 bp),CG at 12 months of age (chromosome2:15 063 190-15 155 309 bp),BL at 24 months of age (chromosome 11:60 727 342-81 425 987 bp),IW at 36 months of age (chromosome 14:15 635 762-15 643 272 bp) and BWG between 12 and 18 months of age (chromosome 26:40 456 192-40 456 477 bp) were identified,respectively.8 genes (BMP10,IFT172,SDC1,TCF23,TRIM54,RAB1A,VPS54 and GDF7) on chromosome 11 were screened out by functional annotation of 186 genes in 5 genomic regions,which were related to bone growth,muscle development and growth regulation.It was suggested that they could be used as candidate genes for growth traits of cattle for further verification.

Key words: Nanyang cattle; growth traits; SLAF-seq; GWAS; candidate gene

CLC Number:

S813.3

LYU Shijie, CHEN Fuying, ZHANG Zijing, SHI Qiaoting, XIN Xiaoling, CHU Qiuxia, LI Wenjun, BAI Zhonglin, WANG Eryao, XU Zhaoxue. Genomic Regions Associated with Growth Traits in Nanyang Cattle Using Genome-wide Association Analysis[J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(1): 74-82.

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Genomic Regions Associated with Growth Traits in Nanyang Cattle Using Genome-wide Association Analysis

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