思州鸡BMP15基因第1外显子多态性及其与蛋品质的关联分析

doi:10.16431/j.cnki.1671-7236.2019.12.028

Abstract

Abstract: This study was aimed to explore the polymorphisms of BMP15 gene exon 1 and its association with egg quality in Sizhou chicken.The direct sequencing of PCR products was adopted to detect the polymorphism of BMP15 gene exon 1 in Sizhou chicken.After direct sequencing,sequence adjusting were conducted using DNAStar software.Data of genotype frequency was counted by Excel 2007 and analyzed by χ² test.The association of genotype and egg quality were analyzed with SPSS 18.0.The results showed that BMP15 gene exon 1 in Sizhou chicken had four SNPs:397C/T,473A/G,603C/T and 612C/T,which were not in Hardy-Weinberg equilibrium (P<0.05);The polymorphic information content (PIC) of 397C/T and 603C/T loci were 0.3488 and 0.3299,respectively,belonging to moderate polymorphism (0.25 < PIC < 0.5).The PIC of 473A/G and 612C/T loci were 0.1527 and 0.2075,respectively,belonging to low polymorphism (PIC<0.25).The results of correlation analysis showed that the 397C/T locus significantly affected the protein height,Hastelloy unit and egg yolk weight of Sizhou chicken (P<0.05);There were no significant difference of 473A/G and 603C/T loci with egg quality in Sizhou chicken (P>0.05);The 612C/T locus had a significant influence on egg length of Sizhou chicken,respectively (P<0.05).This results suggested that BMP15 gene exon 1 had a larger genetic effect on the protein height and egg length in Sizhou chicken.BMP15 gene might be one of the genes affecting the egg quality in Sizhou chicken,and could be used as a molecular marker for egg quality traits such as protein height and egg length and diameter in Sizhou chicken.

Key words: Sizhou chicken; BMP15 gene; polymorphism; egg quality

CLC Number:

S813.3

LI Honglin, LI Wei, ZHANG Fuping, ZU Panyu, MU Tenghui, LIU Yang, LONG Guangli, LI Kaiyang, LIN Jiadong. Polymorphisms of BMP15 Gene Exon 1 and Its Association with Egg Quality in Sizhou Chicken[J]. China Animal Husbandry and Veterinary Medicine, 2019, 46(12): 3690-3697.

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Polymorphisms of BMP15 Gene Exon 1 and Its Association with Egg Quality in Sizhou Chicken

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