China Animal Husbandry & Veterinary Medicine ›› 2009, Vol. 36 ›› Issue (5): 107-111.

• 遗传繁育 • Previous Articles     Next Articles

PCR-SSCP Detection of Ghrelin and Pit-1 Gene in Chaohu Duck

LI Jun-ying1, ZHAN Kai1, XU Yue-ying1, WANG De-sheng2, ZHAO Rui-hong1, TANG Zhong-jie3   

  1. (1.Institute of Animal Husbandry and Veterinary, Agricultural Academy of Anhui Province, Hefei 230031, China;2.Haian Town Livestock Farm Veterinary Station of Haian County of Jiangsu Province, Haian 226600, China;3.Chaohu Duck Breeding Farm of Lujiang County of Anhui Province, Lujiang 231490, China)
  • Received:1900-01-01 Revised:1900-01-01 Online:2009-05-20 Published:2009-05-20
  • Contact: ZHAN Kai

Abstract: Ghrelin gene and pituitary specific transcription factor 1 (Pit-1) gene were studied as candidate genes on the Chaohu duck in this study. Polymorphisms of Ghrelin and Pit-1 gene had been analyzed by PCR-SSCP and DNA sequencing method. The results showed that three SNPs were identified at Ghrelin gene, one SNP (G54A) located at the third exon, another two in the fifth exon were transitions of C to A (149) and G to T (166). The frequency of A/B alleles for Ghrelin gene in the population was 0.49 and 0.51, and that of M/N alleles was 0.19 and 0.81. No polymorphisms were detected in the amplified regions of two pairs of primers in Chaohu duck population tested, which may indicate that the sequences of exons 3 and 4 of Pit-1 gene were rather conserved.

Key words: Chaohu duck; Ghrelin gene; Pit-1 gene; SNPs

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