抗奶牛热应激中药组方筛选及应用研究

doi:10.16431/j.cnki.1671-7236.2024.11.041

Abstract

Abstract: 【Objective】 This study was aimed to screening traditional Chinese medicine formulas and their applicable dosages for anti-heat stress in dairy cows,and providing reference for traditional Chinese medicine in anti-heat stress in dairy cows. 【Method】 With the principle of “supplementing qi and nourishing blood,nourishing yin and generating fluids” and “clearing heat and cooling blood”,中国畜牧兽医51卷 11期王晓芳等：抗奶牛热应激中药组方筛选及应用研究 three traditional Chinese medicine formulas were formulated,which were named TCM1，TCM2 and TCM3,respectively.40 dairy cows with similar milk production and milk composition were selected,and randomly divided into 4 groups TM1,TM2,TM3 and control (CON) groups (n＝10).Dairy cows in CON group were fed with conventional diet,while in the other three groups were fed with diets supplemented with TCM1,TCM2 and TCM3,respectively.On the 1st,7th,14th,21st,28th and 30th day of the experiment,the milk production of each group of dairy cows was recorded,and the milk fat percentage,milk protein percentage,lactose percentage,and somatic cell number in milk were measured to screen for the best traditional Chinese medicine formula.Based on the selected formula,40 healthy dairy cows with similar milk production and milk indicators were selected and randomly divided into high-dose group (30 g/(head·d)),medium dose group (20 g/(head·d)), low-dose group (15 g/(head·d)) and control group (n＝10).Dairy cows in control group were fed with conventional diet,while each traditional Chinese medicine group was fed with a diet with different doses of traditional Chinese medicine,respectively.The milk production of each cow was recorded on the 1st,15th,31st day of the experiment.The milk samples were collected from each group,and the milk fat percentage,milk protein percentage,lactose percentage,and somatic cell number were measured for screening the optimal dosage of medication. 【Result】 Results of formula screening test:①The results of milk fat percentage measurement showed that,On the 21st day,the milk fat percentage in TCM1 group was significantly higher than of CON and TCM2 groups (P＜0.05),and there was no significant difference compared to TCM3 group (P＞0.05).On the 28th day,the milk fat percentage in TCM1，TCM2 and TCM3 groups was significantly higher than that of CON group (P＜0.05)，and there was no significant difference among them (P＞0.05).On the 30th day,the milk fat percentage of TCM1 and TCM3 groups was significantly higher than that of TCM2 and CON groups.The milk protein percentage in TCM1 was significantly higher than that of CON group (P＜0.05) on the 14th and 21th day.And there were no significant differences between the experimental groups at other time points.There was no significant difference in lactose percentage among the groups during the experiment (P＞0.05).②The results of milk production measurement showed that,on the 14th,21st,28th and 30th day,milk production in all traditional Chinese medicine groups was significantly higher than that of CON group (P＜0.05).On the 28th day,milk production in TCM3 group was significantly higher than that in TCM2 group (P＜0.05).The test results of 4% FCM showed that,at the 14th day and subsequent time points,4% FCM of all traditional Chinese medicine groups showed a significantly higher level than that of CON group (P＜0.05).On the 14th day,TCM3 group was significantly higher than that of TCM1 group (P＜0.05).On the 28th and 30th day,4% FCM of TCM3 group was significantly higher than that of TCM2 group (P＜0.05).③The results of somatic cell count (SCC) measurement showed that,on the 14th day,the SCC of TCM3 group was significantly lower than that of CON group (P＜0.05).On the 21st,28th and 30th day,the SCC of TCM3 group was significantly lower than that of TCM1 group.On the 21st and 30th day,the SCC of TCM2 group was significantly lower than that of TCM1 group (P＜0.05).The dose screening results showed that:①There were no significant difference in milk fat percentage,milk protein percentage,lactose percentage among all the groups (P＞0.05).② The results of milk production level measurement showed that,on the 15th day,the actual milk production of medium and high dose groups were significantly higher than that of low dose group and control group (P＜0.05),and there was no significant difference in the 4% FCM among all the groups (P＞0.05).On the 31st day,the actual milk production and 4% FCM of medium and high dose groups were significantly higher than that of control group (P＜0.05).③ The results of SCC measurement showed that,on the 15th day,each SCC of medium and high dose groups was significantly lower than that of low dose group and control group (P＜0.05).On the 31st day,each SCC of all traditional Chinese medicine groups was significantly lower than that of control group (P＜0.05),and SCC of medium and high dose groups were significantly lower than that of low dose group (P＜0.05). 【Conclusion】 The traditional Chinese medicine formula selected with the best anti-heat stress effect on dairy cows was TCM3.The recommended amount of additives was 20 g/(head·d).

Key words: dairy cows; heat stress; traditional Chinese medicine; screening; application

CLC Number:

S852.4

WANG Xiaofang, WANG Yawen, LI Jiefeng, FU Le, ZHANG Ning, QIN Jianhua. Screening and Application Research of Traditional Chinese Medicine Formulas for Anti-heat Stress in Dairy Cows[J]. China Animal Husbandry and Veterinary Medicine, 2024, 51(11): 5064-5073.

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Screening and Application Research of Traditional Chinese Medicine Formulas for Anti-heat Stress in Dairy Cows

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