随机引物PCR技术及其在动物医学中应用的研究进展

Abstract

Abstract: Arbitrarily primed polymerase chain reaction (AP-PCR) is a PCR based technique which detects DNA-sequence polymorphisms. The technique involves the use of single arbitrary short primers in a DNA amplification and requires no prior knowledge of the molecular biology of the organisms to be investigated. It is a simple,efficient and practical molecular technique and is widely applied in many fields of molecular biology. The review gave emphasis on principle and procedure about AP-PCR,its advantages,disadvantages and applications in veterinary medicine.

Key words: arbitrarily primed PCR; research progress; veterinary medicine; application

CLC Number:

SHEN Xue-huai, ZHANG Dan-jun, PAN Xiao-cheng, ZHAO Rui-hong, HU Xiao-miao, DAI Yin, HOU Hong-yan, ZHOU Xue-li, ZHU Chuan-min. Research Progress of Arbitrarily Primed Polymerase Chain Reaction and its Application in Veterinary Medicine[J]. , 2014, 41(10): 62-68.

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Research Progress of Arbitrarily Primed Polymerase Chain Reaction and its Application in Veterinary Medicine

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